Strain Fitness in Bacteroides thetaiotaomicron VPI-5482 around BT1221

Experiment: Varel_Bryant medium with Zinc Pyrithione 0.05 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBT1219 and BT1220 are separated by 184 nucleotidesBT1220 and BT1221 are separated by 23 nucleotidesBT1221 and BT1222 are separated by 344 nucleotides BT1219: BT1219 - conserved hypothetical protein (NCBI ptt file), at 1,516,364 to 1,517,314 BT1219 BT1220: BT1220 - 6-phosphogluconolactonase (NCBI ptt file), at 1,517,499 to 1,518,188 BT1220 BT1221: BT1221 - glucose-6-phosphate 1-dehydrogenase (NCBI ptt file), at 1,518,212 to 1,519,708 BT1221 BT1222: BT1222 - 6-phosphogluconate dehydrogenase,decarboxylating (NCBI ptt file), at 1,520,053 to 1,521,528 BT1222 Position (kb) 1518 1519 1520Strain fitness (log2 ratio) -2 -1 0 1 2at 1517.230 kb on - strandat 1517.237 kb on - strandat 1517.241 kb on - strandat 1517.314 kb on + strandat 1517.322 kb on + strandat 1517.348 kb on + strandat 1517.365 kb on + strandat 1517.390 kb on + strandat 1517.391 kb on - strandat 1517.395 kb on - strandat 1517.395 kb on - strandat 1517.395 kb on - strandat 1517.447 kb on - strandat 1517.461 kb on + strandat 1517.462 kb on - strandat 1517.463 kb on + strandat 1517.465 kb on + strandat 1517.465 kb on + strandat 1517.466 kb on - strandat 1517.466 kb on - strandat 1517.469 kb on - strandat 1517.469 kb on - strandat 1517.470 kb on + strandat 1517.481 kb on + strandat 1517.515 kb on + strandat 1517.516 kb on - strandat 1517.568 kb on + strandat 1517.569 kb on - strand, within BT1220at 1517.571 kb on - strand, within BT1220at 1517.571 kb on - strand, within BT1220at 1517.572 kb on + strandat 1517.573 kb on - strand, within BT1220at 1517.775 kb on - strand, within BT1220at 1517.783 kb on + strandat 1517.784 kb on - strand, within BT1220at 1517.784 kb on - strand, within BT1220at 1517.929 kb on + strand, within BT1220at 1517.974 kb on + strand, within BT1220at 1517.979 kb on - strand, within BT1220at 1518.050 kb on + strandat 1518.115 kb on - strand, within BT1220at 1518.226 kb on - strandat 1518.289 kb on + strandat 1518.290 kb on - strandat 1518.290 kb on - strandat 1518.290 kb on - strandat 1518.334 kb on - strandat 1518.369 kb on - strand, within BT1221at 1518.423 kb on - strand, within BT1221at 1518.423 kb on - strand, within BT1221at 1518.465 kb on + strand, within BT1221at 1518.574 kb on + strand, within BT1221at 1518.575 kb on - strand, within BT1221at 1518.773 kb on - strand, within BT1221at 1518.773 kb on - strand, within BT1221at 1518.774 kb on + strandat 1518.844 kb on + strand, within BT1221at 1518.845 kb on - strand, within BT1221at 1518.862 kb on + strandat 1518.907 kb on - strand, within BT1221at 1518.917 kb on + strandat 1519.279 kb on + strand, within BT1221at 1519.375 kb on + strand, within BT1221at 1519.396 kb on + strand, within BT1221at 1519.518 kb on - strand, within BT1221at 1519.546 kb on - strand, within BT1221at 1519.598 kb on - strandat 1519.636 kb on - strandat 1519.735 kb on + strandat 1519.838 kb on + strandat 1519.838 kb on + strandat 1519.848 kb on + strandat 1520.056 kb on + strandat 1520.056 kb on + strandat 1520.057 kb on - strandat 1520.061 kb on + strandat 1520.061 kb on + strandat 1520.063 kb on + strandat 1520.063 kb on + strandat 1520.063 kb on + strandat 1520.148 kb on + strandat 1520.148 kb on + strandat 1520.327 kb on + strand, within BT1222at 1520.327 kb on + strandat 1520.380 kb on + strandat 1520.380 kb on + strand, within BT1222at 1520.562 kb on + strandat 1520.565 kb on + strand, within BT1222at 1520.571 kb on + strand, within BT1222at 1520.580 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Varel_Bryant medium with Zinc Pyrithione 0.05 mM
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1,517,230 - +0.2
1,517,237 - -1.5
1,517,241 - -1.3
1,517,314 + +0.6
1,517,322 + -0.5
1,517,348 + +1.1
1,517,365 + -0.5
1,517,390 + -1.5
1,517,391 - +0.6
1,517,395 - -0.2
1,517,395 - -0.5
1,517,395 - +0.9
1,517,447 - -0.9
1,517,461 + -0.4
1,517,462 - -0.2
1,517,463 + -0.3
1,517,465 + -0.3
1,517,465 + -2.0
1,517,466 - +0.5
1,517,466 - +1.6
1,517,469 - -1.6
1,517,469 - +0.3
1,517,470 + -0.2
1,517,481 + -0.5
1,517,515 + -0.5
1,517,516 - +1.1
1,517,568 + +0.1
1,517,569 - BT1220 0.10 -0.3
1,517,571 - BT1220 0.10 +0.5
1,517,571 - BT1220 0.10 +1.1
1,517,572 + +1.1
1,517,573 - BT1220 0.11 +1.1
1,517,775 - BT1220 0.40 -0.1
1,517,783 + +0.1
1,517,784 - BT1220 0.41 +2.1
1,517,784 - BT1220 0.41 -0.5
1,517,929 + BT1220 0.62 +1.1
1,517,974 + BT1220 0.69 +1.1
1,517,979 - BT1220 0.70 +1.1
1,518,050 + -0.5
1,518,115 - BT1220 0.89 +1.1
1,518,226 - +1.1
1,518,289 + +1.1
1,518,290 - +1.1
1,518,290 - +1.1
1,518,290 - +1.1
1,518,334 - -0.5
1,518,369 - BT1221 0.10 +1.1
1,518,423 - BT1221 0.14 +0.1
1,518,423 - BT1221 0.14 -0.9
1,518,465 + BT1221 0.17 +0.1
1,518,574 + BT1221 0.24 +1.1
1,518,575 - BT1221 0.24 -0.6
1,518,773 - BT1221 0.37 -0.9
1,518,773 - BT1221 0.37 +0.1
1,518,774 + +0.5
1,518,844 + BT1221 0.42 +1.1
1,518,845 - BT1221 0.42 -0.8
1,518,862 + +1.1
1,518,907 - BT1221 0.46 +1.1
1,518,917 + +1.1
1,519,279 + BT1221 0.71 +0.1
1,519,375 + BT1221 0.78 -0.8
1,519,396 + BT1221 0.79 +0.1
1,519,518 - BT1221 0.87 +0.1
1,519,546 - BT1221 0.89 -0.3
1,519,598 - +0.1
1,519,636 - -0.7
1,519,735 + -0.9
1,519,838 + +2.1
1,519,838 + +0.5
1,519,848 + -1.5
1,520,056 + +0.5
1,520,056 + +1.1
1,520,057 - -0.3
1,520,061 + +2.6
1,520,061 + +2.1
1,520,063 + -0.5
1,520,063 + +1.1
1,520,063 + +0.1
1,520,148 + +2.6
1,520,148 + -0.5
1,520,327 + BT1222 0.19 -0.9
1,520,327 + +1.1
1,520,380 + +1.1
1,520,380 + BT1222 0.22 +0.1
1,520,562 + +1.1
1,520,565 + BT1222 0.35 -0.5
1,520,571 + BT1222 0.35 +1.1
1,520,580 + +2.1

Or see this region's nucleotide sequence