Experiment: Varel_Bryant medium with Zinc Pyrithione 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT0545 and BT0546 are separated by 151 nucleotides BT0546 and BT0547 are separated by 74 nucleotides BT0547 and BT0548 are separated by 57 nucleotides
BT0545: BT0545 - nitrogen regulatory protein P-II (NCBI ptt file), at 671,772 to 672,128
BT0545
BT0546: BT0546 - conserved hypothetical protein (NCBI ptt file), at 672,280 to 673,005
BT0546
BT0547: BT0547 - aspartate aminotransferase (NCBI ptt file), at 673,080 to 674,312
BT0547
BT0548: BT0548 - diaminopimelate epimerase (NCBI ptt file), at 674,370 to 675,173
BT0548
Position (kb)
673
674
675 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 672.168 kb on + strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.201 kb on - strand at 672.227 kb on + strand at 672.248 kb on + strand at 672.306 kb on - strand at 672.318 kb on + strand at 672.329 kb on - strand at 672.472 kb on + strand, within BT0546 at 672.473 kb on - strand, within BT0546 at 672.531 kb on - strand, within BT0546 at 672.631 kb on + strand, within BT0546 at 672.656 kb on + strand, within BT0546 at 672.759 kb on + strand, within BT0546 at 672.815 kb on + strand, within BT0546 at 672.827 kb on - strand, within BT0546 at 672.873 kb on + strand, within BT0546 at 672.910 kb on + strand, within BT0546 at 673.058 kb on + strand at 673.059 kb on - strand at 673.078 kb on + strand at 673.115 kb on + strand at 673.194 kb on + strand at 673.194 kb on + strand at 673.195 kb on - strand at 673.210 kb on + strand, within BT0547 at 673.271 kb on - strand, within BT0547 at 673.393 kb on + strand at 673.530 kb on - strand, within BT0547 at 673.648 kb on + strand, within BT0547 at 673.699 kb on + strand, within BT0547 at 673.835 kb on - strand, within BT0547 at 673.899 kb on - strand, within BT0547 at 673.908 kb on - strand, within BT0547 at 673.909 kb on + strand, within BT0547 at 673.922 kb on + strand, within BT0547 at 673.943 kb on + strand, within BT0547 at 674.055 kb on + strand, within BT0547 at 674.058 kb on - strand, within BT0547 at 674.060 kb on + strand, within BT0547 at 674.080 kb on + strand, within BT0547 at 674.111 kb on + strand, within BT0547 at 674.118 kb on - strand, within BT0547 at 674.156 kb on + strand, within BT0547 at 674.312 kb on + strand at 674.313 kb on - strand at 674.347 kb on - strand at 674.351 kb on - strand at 674.357 kb on + strand at 674.364 kb on + strand at 674.473 kb on - strand, within BT0548 at 674.475 kb on + strand, within BT0548 at 674.622 kb on + strand, within BT0548 at 674.696 kb on + strand, within BT0548 at 674.808 kb on + strand, within BT0548 at 674.809 kb on - strand, within BT0548 at 674.914 kb on + strand, within BT0548 at 675.118 kb on + strand at 675.119 kb on - strand at 675.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Varel_Bryant medium with Zinc Pyrithione 0.05 mM remove 672,168 + -0.2 672,201 - -0.7 672,201 - -0.3 672,201 - -0.4 672,227 + -0.2 672,248 + -1.1 672,306 - +2.9 672,318 + -0.5 672,329 - -0.7 672,472 + BT0546 0.26 -0.1 672,473 - BT0546 0.27 -1.1 672,531 - BT0546 0.35 -1.1 672,631 + BT0546 0.48 -3.0 672,656 + BT0546 0.52 -0.5 672,759 + BT0546 0.66 -2.1 672,815 + BT0546 0.74 -1.1 672,827 - BT0546 0.75 +0.3 672,873 + BT0546 0.82 +0.6 672,910 + BT0546 0.87 -0.4 673,058 + -0.7 673,059 - -1.1 673,078 + +0.3 673,115 + +0.3 673,194 + -0.1 673,194 + -0.1 673,195 - -0.7 673,210 + BT0547 0.11 -0.1 673,271 - BT0547 0.15 +0.9 673,393 + +0.9 673,530 - BT0547 0.36 -1.1 673,648 + BT0547 0.46 -0.8 673,699 + BT0547 0.50 -1.0 673,835 - BT0547 0.61 +0.9 673,899 - BT0547 0.66 +1.4 673,908 - BT0547 0.67 -1.0 673,909 + BT0547 0.67 -0.1 673,922 + BT0547 0.68 -1.5 673,943 + BT0547 0.70 +2.4 674,055 + BT0547 0.79 -0.1 674,058 - BT0547 0.79 -0.1 674,060 + BT0547 0.79 -0.1 674,080 + BT0547 0.81 -0.3 674,111 + BT0547 0.84 -0.7 674,118 - BT0547 0.84 -1.1 674,156 + BT0547 0.87 -0.3 674,312 + -0.9 674,313 - -0.7 674,347 - +0.9 674,351 - -0.5 674,357 + -2.5 674,364 + -1.5 674,473 - BT0548 0.13 -0.5 674,475 + BT0548 0.13 +0.9 674,622 + BT0548 0.31 +0.4 674,696 + BT0548 0.41 -1.1 674,808 + BT0548 0.54 +0.9 674,809 - BT0548 0.55 -1.7 674,914 + BT0548 0.68 +0.4 675,118 + +0.2 675,119 - +0.3 675,120 + +0.4
Or see this region's nucleotide sequence