Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5296 and Pf6N2E2_5297 are separated by 93 nucleotides Pf6N2E2_5297 and Pf6N2E2_5298 are separated by 30 nucleotides
Pf6N2E2_5296 - Threonine synthase (EC 4.2.3.1), at 6,089,877 to 6,091,130
_5296
Pf6N2E2_5297 - RidA/YER057c/UK114 superfamily, group 2, YoaB-like protein, at 6,091,224 to 6,091,655
_5297
Pf6N2E2_5298 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 6,091,686 to 6,092,858
_5298
Position (kb)
6091
6092 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6090.283 kb on + strand, within Pf6N2E2_5296 at 6090.283 kb on + strand, within Pf6N2E2_5296 at 6090.284 kb on - strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.373 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.487 kb on + strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.488 kb on - strand, within Pf6N2E2_5296 at 6090.514 kb on + strand, within Pf6N2E2_5296 at 6090.515 kb on - strand, within Pf6N2E2_5296 at 6090.515 kb on - strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.565 kb on + strand, within Pf6N2E2_5296 at 6090.566 kb on - strand, within Pf6N2E2_5296 at 6090.599 kb on - strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.646 kb on + strand, within Pf6N2E2_5296 at 6090.647 kb on - strand, within Pf6N2E2_5296 at 6090.647 kb on - strand, within Pf6N2E2_5296 at 6090.865 kb on + strand, within Pf6N2E2_5296 at 6090.865 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.934 kb on + strand, within Pf6N2E2_5296 at 6090.935 kb on - strand, within Pf6N2E2_5296 at 6090.935 kb on - strand, within Pf6N2E2_5296 at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.051 kb on + strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.052 kb on - strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.054 kb on + strand at 6091.055 kb on - strand at 6091.055 kb on - strand at 6091.055 kb on - strand at 6091.075 kb on + strand at 6091.075 kb on + strand at 6091.076 kb on - strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.104 kb on + strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.105 kb on - strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.108 kb on + strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.109 kb on - strand at 6091.117 kb on + strand at 6091.146 kb on + strand at 6091.147 kb on - strand at 6091.147 kb on - strand at 6091.319 kb on + strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.319 kb on - strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.519 kb on + strand, within Pf6N2E2_5297 at 6091.520 kb on - strand, within Pf6N2E2_5297 at 6091.558 kb on + strand, within Pf6N2E2_5297 at 6091.558 kb on + strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.559 kb on - strand, within Pf6N2E2_5297 at 6091.562 kb on + strand, within Pf6N2E2_5297 at 6091.563 kb on - strand, within Pf6N2E2_5297 at 6091.655 kb on + strand at 6091.656 kb on - strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.671 kb on + strand at 6091.672 kb on - strand at 6091.765 kb on + strand at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6091.886 kb on + strand, within Pf6N2E2_5298 at 6092.140 kb on + strand, within Pf6N2E2_5298 at 6092.141 kb on - strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.181 kb on + strand, within Pf6N2E2_5298 at 6092.395 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.443 kb on + strand, within Pf6N2E2_5298 at 6092.477 kb on - strand, within Pf6N2E2_5298 at 6092.613 kb on + strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298 at 6092.614 kb on - strand, within Pf6N2E2_5298
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 6,090,283 + Pf6N2E2_5296 0.32 +0.2 6,090,283 + Pf6N2E2_5296 0.32 -0.0 6,090,284 - Pf6N2E2_5296 0.32 +0.4 6,090,373 + Pf6N2E2_5296 0.40 -0.4 6,090,373 + Pf6N2E2_5296 0.40 +0.5 6,090,373 + Pf6N2E2_5296 0.40 +0.2 6,090,487 + Pf6N2E2_5296 0.49 +1.1 6,090,487 + Pf6N2E2_5296 0.49 -1.2 6,090,487 + Pf6N2E2_5296 0.49 -2.3 6,090,487 + Pf6N2E2_5296 0.49 +0.1 6,090,487 + Pf6N2E2_5296 0.49 +0.8 6,090,487 + Pf6N2E2_5296 0.49 +0.9 6,090,487 + Pf6N2E2_5296 0.49 +0.0 6,090,487 + Pf6N2E2_5296 0.49 -1.2 6,090,488 - Pf6N2E2_5296 0.49 -0.4 6,090,488 - Pf6N2E2_5296 0.49 -0.3 6,090,488 - Pf6N2E2_5296 0.49 +0.2 6,090,488 - Pf6N2E2_5296 0.49 +0.0 6,090,488 - Pf6N2E2_5296 0.49 -0.1 6,090,514 + Pf6N2E2_5296 0.51 +0.4 6,090,515 - Pf6N2E2_5296 0.51 +0.9 6,090,515 - Pf6N2E2_5296 0.51 +0.9 6,090,565 + Pf6N2E2_5296 0.55 -0.4 6,090,565 + Pf6N2E2_5296 0.55 -0.3 6,090,565 + Pf6N2E2_5296 0.55 +0.2 6,090,566 - Pf6N2E2_5296 0.55 +0.2 6,090,599 - Pf6N2E2_5296 0.58 -0.1 6,090,646 + Pf6N2E2_5296 0.61 -1.2 6,090,646 + Pf6N2E2_5296 0.61 +1.0 6,090,646 + Pf6N2E2_5296 0.61 -0.5 6,090,646 + Pf6N2E2_5296 0.61 +0.8 6,090,647 - Pf6N2E2_5296 0.61 +0.3 6,090,647 - Pf6N2E2_5296 0.61 -0.5 6,090,865 + Pf6N2E2_5296 0.79 -0.2 6,090,865 + Pf6N2E2_5296 0.79 -0.6 6,090,934 + Pf6N2E2_5296 0.84 +0.8 6,090,934 + Pf6N2E2_5296 0.84 +0.9 6,090,934 + Pf6N2E2_5296 0.84 +0.3 6,090,935 - Pf6N2E2_5296 0.84 -2.2 6,090,935 - Pf6N2E2_5296 0.84 +0.0 6,091,051 + +0.2 6,091,051 + +1.1 6,091,051 + +0.2 6,091,051 + +0.8 6,091,051 + -0.0 6,091,051 + +0.5 6,091,052 - -0.2 6,091,052 - +0.1 6,091,052 - +1.3 6,091,052 - -0.0 6,091,054 + -0.9 6,091,054 + +0.4 6,091,054 + +0.3 6,091,054 + +0.7 6,091,054 + +1.1 6,091,054 + -0.0 6,091,054 + +0.3 6,091,054 + +0.1 6,091,054 + +0.7 6,091,055 - -0.4 6,091,055 - -0.0 6,091,055 - -0.3 6,091,075 + -1.0 6,091,075 + +0.7 6,091,076 - -2.4 6,091,104 + +0.3 6,091,104 + +0.1 6,091,104 + +0.4 6,091,104 + +0.2 6,091,104 + +1.1 6,091,104 + -0.2 6,091,104 + +0.1 6,091,104 + +0.8 6,091,104 + -0.6 6,091,104 + +1.8 6,091,104 + -0.5 6,091,105 - -0.2 6,091,105 - +0.4 6,091,105 - -0.5 6,091,105 - -0.1 6,091,105 - +0.0 6,091,105 - +0.1 6,091,105 - -0.5 6,091,105 - -0.6 6,091,105 - -0.4 6,091,105 - -1.8 6,091,105 - +0.3 6,091,105 - -0.1 6,091,105 - -0.9 6,091,105 - +0.5 6,091,105 - +0.2 6,091,108 + -1.1 6,091,108 + -0.8 6,091,108 + -0.0 6,091,108 + +0.4 6,091,108 + +0.7 6,091,108 + -0.3 6,091,108 + +0.7 6,091,108 + +2.4 6,091,108 + -0.2 6,091,108 + -0.0 6,091,109 - +0.6 6,091,109 - -0.1 6,091,109 - +0.2 6,091,109 - -0.7 6,091,109 - -1.0 6,091,109 - +0.4 6,091,109 - +0.8 6,091,109 - +0.1 6,091,109 - +0.1 6,091,109 - +0.5 6,091,109 - +0.6 6,091,109 - -0.4 6,091,109 - -0.7 6,091,109 - +0.2 6,091,117 + +0.4 6,091,146 + -0.8 6,091,147 - -0.1 6,091,147 - +0.2 6,091,319 + Pf6N2E2_5297 0.22 -0.5 6,091,319 - Pf6N2E2_5297 0.22 -1.2 6,091,319 - Pf6N2E2_5297 0.22 -2.2 6,091,319 - Pf6N2E2_5297 0.22 +0.3 6,091,519 + Pf6N2E2_5297 0.68 +0.3 6,091,519 + Pf6N2E2_5297 0.68 -0.1 6,091,519 + Pf6N2E2_5297 0.68 +0.9 6,091,519 + Pf6N2E2_5297 0.68 -0.4 6,091,520 - Pf6N2E2_5297 0.69 -0.3 6,091,558 + Pf6N2E2_5297 0.77 +0.4 6,091,558 + Pf6N2E2_5297 0.77 -0.5 6,091,559 - Pf6N2E2_5297 0.78 +0.2 6,091,559 - Pf6N2E2_5297 0.78 +0.2 6,091,559 - Pf6N2E2_5297 0.78 +2.7 6,091,559 - Pf6N2E2_5297 0.78 -0.9 6,091,559 - Pf6N2E2_5297 0.78 -0.0 6,091,559 - Pf6N2E2_5297 0.78 +1.1 6,091,562 + Pf6N2E2_5297 0.78 +0.0 6,091,563 - Pf6N2E2_5297 0.78 +0.4 6,091,655 + +1.5 6,091,656 - +0.3 6,091,671 + +0.5 6,091,671 + -0.8 6,091,671 + -0.1 6,091,671 + -0.5 6,091,671 + -0.7 6,091,671 + +0.1 6,091,671 + -0.3 6,091,672 - +0.2 6,091,765 + -0.2 6,091,886 + Pf6N2E2_5298 0.17 +1.1 6,091,886 + Pf6N2E2_5298 0.17 +0.6 6,092,140 + Pf6N2E2_5298 0.39 -0.0 6,092,141 - Pf6N2E2_5298 0.39 +0.4 6,092,181 + Pf6N2E2_5298 0.42 -1.3 6,092,181 + Pf6N2E2_5298 0.42 +0.7 6,092,395 + Pf6N2E2_5298 0.60 +0.7 6,092,443 + Pf6N2E2_5298 0.65 -0.1 6,092,443 + Pf6N2E2_5298 0.65 -0.3 6,092,477 - Pf6N2E2_5298 0.67 +0.3 6,092,613 + Pf6N2E2_5298 0.79 +0.8 6,092,614 - Pf6N2E2_5298 0.79 -0.8 6,092,614 - Pf6N2E2_5298 0.79 +0.7
Or see this region's nucleotide sequence