Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4535 and Pf6N2E2_4536 overlap by 4 nucleotides Pf6N2E2_4536 and Pf6N2E2_4537 are separated by 9 nucleotides Pf6N2E2_4537 and Pf6N2E2_4538 overlap by 1 nucleotides Pf6N2E2_4538 and Pf6N2E2_4539 are separated by 15 nucleotides
Pf6N2E2_4535 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 5,269,316 to 5,270,419
_4535
Pf6N2E2_4536 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 5,270,416 to 5,271,069
_4536
Pf6N2E2_4537 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 5,271,079 to 5,271,333
_4537
Pf6N2E2_4538 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 5,271,333 to 5,272,130
_4538
Pf6N2E2_4539 - Type III secretion inner membrane protein (YscU,SpaS,EscU,HrcU,SsaU, homologous to flagellar export components), at 5,272,146 to 5,273,246
_4539
Position (kb) 5271
5272 Strain fitness (log2 ratio) -1
0
1
2 at 5270.136 kb on - strand, within Pf6N2E2_4535 at 5270.330 kb on + strand at 5270.330 kb on + strand at 5270.331 kb on - strand at 5270.494 kb on - strand, within Pf6N2E2_4536 at 5270.517 kb on - strand, within Pf6N2E2_4536 at 5270.584 kb on + strand, within Pf6N2E2_4536 at 5270.585 kb on - strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.902 kb on + strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5270.903 kb on - strand, within Pf6N2E2_4536 at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.055 kb on + strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.056 kb on - strand at 5271.061 kb on + strand at 5271.061 kb on + strand at 5271.061 kb on + strand at 5271.100 kb on - strand at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.167 kb on + strand, within Pf6N2E2_4537 at 5271.328 kb on - strand at 5271.357 kb on + strand at 5271.403 kb on - strand at 5271.414 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.621 kb on + strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.622 kb on - strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.798 kb on + strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.964 kb on - strand, within Pf6N2E2_4538 at 5271.965 kb on + strand, within Pf6N2E2_4538 at 5272.039 kb on - strand, within Pf6N2E2_4538
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 5,270,136 - Pf6N2E2_4535 0.74 +0.0 5,270,330 + -0.2 5,270,330 + +0.1 5,270,331 - +0.0 5,270,494 - Pf6N2E2_4536 0.12 -0.4 5,270,517 - Pf6N2E2_4536 0.15 +0.9 5,270,584 + Pf6N2E2_4536 0.26 -0.5 5,270,585 - Pf6N2E2_4536 0.26 +0.9 5,270,902 + Pf6N2E2_4536 0.74 +2.0 5,270,902 + Pf6N2E2_4536 0.74 -1.3 5,270,902 + Pf6N2E2_4536 0.74 -0.0 5,270,902 + Pf6N2E2_4536 0.74 -0.4 5,270,902 + Pf6N2E2_4536 0.74 -0.2 5,270,902 + Pf6N2E2_4536 0.74 +0.5 5,270,902 + Pf6N2E2_4536 0.74 -0.9 5,270,902 + Pf6N2E2_4536 0.74 -0.2 5,270,903 - Pf6N2E2_4536 0.74 +0.4 5,270,903 - Pf6N2E2_4536 0.74 -1.0 5,270,903 - Pf6N2E2_4536 0.74 +0.8 5,270,903 - Pf6N2E2_4536 0.74 +0.5 5,270,903 - Pf6N2E2_4536 0.74 -0.1 5,270,903 - Pf6N2E2_4536 0.74 -0.1 5,270,903 - Pf6N2E2_4536 0.74 -0.4 5,271,055 + -0.5 5,271,055 + -1.1 5,271,055 + +0.1 5,271,055 + -0.8 5,271,056 - +0.4 5,271,056 - -0.4 5,271,056 - -1.4 5,271,056 - +0.4 5,271,056 - -1.6 5,271,061 + -1.1 5,271,061 + -0.2 5,271,061 + +0.5 5,271,100 - +0.2 5,271,167 + Pf6N2E2_4537 0.35 -1.4 5,271,167 + Pf6N2E2_4537 0.35 -0.7 5,271,328 - -1.0 5,271,357 + -0.5 5,271,403 - +0.6 5,271,414 + Pf6N2E2_4538 0.10 +0.3 5,271,621 + Pf6N2E2_4538 0.36 -0.6 5,271,621 + Pf6N2E2_4538 0.36 -0.9 5,271,621 + Pf6N2E2_4538 0.36 -0.0 5,271,622 - Pf6N2E2_4538 0.36 -0.1 5,271,622 - Pf6N2E2_4538 0.36 +1.3 5,271,798 + Pf6N2E2_4538 0.58 -1.2 5,271,798 + Pf6N2E2_4538 0.58 -0.6 5,271,798 + Pf6N2E2_4538 0.58 -0.1 5,271,798 + Pf6N2E2_4538 0.58 +0.2 5,271,798 + Pf6N2E2_4538 0.58 +0.2 5,271,964 - Pf6N2E2_4538 0.79 -0.5 5,271,964 - Pf6N2E2_4538 0.79 -0.3 5,271,964 - Pf6N2E2_4538 0.79 +0.9 5,271,965 + Pf6N2E2_4538 0.79 +1.3 5,272,039 - Pf6N2E2_4538 0.88 +0.0
Or see this region's nucleotide sequence