Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2165 and Pf6N2E2_2166 are separated by 26 nucleotides Pf6N2E2_2166 and Pf6N2E2_2167 overlap by 4 nucleotides Pf6N2E2_2167 and Pf6N2E2_2168 are separated by 25 nucleotides Pf6N2E2_2168 and Pf6N2E2_2169 are separated by 48 nucleotides
Pf6N2E2_2165 - Uncharacterized conserved protein, at 2,570,146 to 2,571,066
_2165
Pf6N2E2_2166 - protein of unknown function DUF6, transmembrane, at 2,571,093 to 2,572,052
_2166
Pf6N2E2_2167 - hypothetical protein, at 2,572,049 to 2,573,326
_2167
Pf6N2E2_2168 - Probable glutathione S-transferase (EC 2.5.1.18), YfcF homolog, at 2,573,352 to 2,573,987
_2168
Pf6N2E2_2169 - Cysteinyl-tRNA synthetase (EC 6.1.1.16), at 2,574,036 to 2,575,406
_2169
Position (kb)
2572
2573
2574 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2571.054 kb on + strand at 2571.062 kb on + strand at 2571.063 kb on - strand at 2571.171 kb on + strand at 2571.171 kb on + strand at 2571.171 kb on + strand at 2571.172 kb on - strand at 2571.172 kb on - strand at 2571.172 kb on - strand at 2571.259 kb on - strand, within Pf6N2E2_2166 at 2571.259 kb on - strand, within Pf6N2E2_2166 at 2571.498 kb on + strand, within Pf6N2E2_2166 at 2571.498 kb on + strand, within Pf6N2E2_2166 at 2571.498 kb on + strand, within Pf6N2E2_2166 at 2571.498 kb on + strand, within Pf6N2E2_2166 at 2571.498 kb on + strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.499 kb on - strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.500 kb on + strand, within Pf6N2E2_2166 at 2571.501 kb on - strand, within Pf6N2E2_2166 at 2571.501 kb on - strand, within Pf6N2E2_2166 at 2571.501 kb on - strand, within Pf6N2E2_2166 at 2571.502 kb on + strand, within Pf6N2E2_2166 at 2571.502 kb on + strand, within Pf6N2E2_2166 at 2571.502 kb on + strand, within Pf6N2E2_2166 at 2571.502 kb on + strand, within Pf6N2E2_2166 at 2571.503 kb on - strand, within Pf6N2E2_2166 at 2571.503 kb on - strand, within Pf6N2E2_2166 at 2571.535 kb on - strand, within Pf6N2E2_2166 at 2571.535 kb on - strand, within Pf6N2E2_2166 at 2571.718 kb on - strand, within Pf6N2E2_2166 at 2571.745 kb on - strand, within Pf6N2E2_2166 at 2571.745 kb on - strand, within Pf6N2E2_2166 at 2571.838 kb on + strand, within Pf6N2E2_2166 at 2571.839 kb on - strand, within Pf6N2E2_2166 at 2571.839 kb on - strand, within Pf6N2E2_2166 at 2571.839 kb on - strand, within Pf6N2E2_2166 at 2571.981 kb on - strand at 2571.999 kb on + strand at 2572.000 kb on - strand at 2572.000 kb on - strand at 2572.000 kb on - strand at 2572.020 kb on - strand at 2572.020 kb on - strand at 2572.044 kb on - strand at 2572.044 kb on - strand at 2572.044 kb on - strand at 2572.055 kb on + strand at 2572.056 kb on - strand at 2572.056 kb on - strand at 2572.056 kb on - strand at 2572.076 kb on + strand at 2572.115 kb on + strand at 2572.115 kb on + strand at 2572.115 kb on + strand at 2572.115 kb on + strand at 2572.116 kb on - strand at 2572.163 kb on + strand at 2572.164 kb on - strand at 2572.164 kb on - strand at 2572.178 kb on + strand, within Pf6N2E2_2167 at 2572.178 kb on + strand, within Pf6N2E2_2167 at 2572.178 kb on + strand, within Pf6N2E2_2167 at 2572.178 kb on + strand, within Pf6N2E2_2167 at 2572.179 kb on - strand, within Pf6N2E2_2167 at 2572.179 kb on - strand, within Pf6N2E2_2167 at 2572.492 kb on + strand, within Pf6N2E2_2167 at 2572.628 kb on + strand, within Pf6N2E2_2167 at 2572.629 kb on - strand, within Pf6N2E2_2167 at 2572.646 kb on + strand, within Pf6N2E2_2167 at 2572.796 kb on + strand, within Pf6N2E2_2167 at 2572.797 kb on - strand, within Pf6N2E2_2167 at 2572.797 kb on - strand, within Pf6N2E2_2167 at 2572.797 kb on - strand, within Pf6N2E2_2167 at 2572.797 kb on - strand, within Pf6N2E2_2167 at 2572.976 kb on + strand, within Pf6N2E2_2167 at 2572.976 kb on + strand, within Pf6N2E2_2167 at 2572.976 kb on + strand, within Pf6N2E2_2167 at 2572.976 kb on + strand, within Pf6N2E2_2167 at 2572.976 kb on + strand, within Pf6N2E2_2167 at 2572.977 kb on - strand, within Pf6N2E2_2167 at 2572.977 kb on - strand, within Pf6N2E2_2167 at 2572.977 kb on - strand, within Pf6N2E2_2167 at 2572.977 kb on - strand, within Pf6N2E2_2167 at 2572.997 kb on + strand, within Pf6N2E2_2167 at 2572.998 kb on - strand, within Pf6N2E2_2167 at 2572.998 kb on - strand, within Pf6N2E2_2167 at 2573.168 kb on - strand, within Pf6N2E2_2167 at 2573.168 kb on - strand, within Pf6N2E2_2167 at 2573.168 kb on - strand, within Pf6N2E2_2167 at 2573.189 kb on + strand, within Pf6N2E2_2167 at 2573.197 kb on + strand, within Pf6N2E2_2167 at 2573.197 kb on + strand, within Pf6N2E2_2167 at 2573.197 kb on + strand, within Pf6N2E2_2167 at 2573.197 kb on + strand, within Pf6N2E2_2167 at 2573.198 kb on - strand, within Pf6N2E2_2167 at 2573.201 kb on + strand at 2573.201 kb on + strand at 2573.201 kb on + strand at 2573.201 kb on + strand at 2573.202 kb on - strand at 2573.202 kb on - strand at 2573.232 kb on - strand at 2573.232 kb on - strand at 2573.240 kb on + strand at 2573.359 kb on + strand at 2573.359 kb on + strand at 2573.359 kb on + strand at 2573.359 kb on + strand at 2573.359 kb on + strand at 2573.359 kb on + strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.360 kb on - strand at 2573.361 kb on + strand at 2573.361 kb on + strand at 2573.361 kb on + strand at 2573.361 kb on + strand at 2573.362 kb on - strand at 2573.362 kb on - strand at 2573.362 kb on - strand at 2573.362 kb on - strand at 2573.362 kb on - strand at 2573.362 kb on - strand at 2573.445 kb on + strand, within Pf6N2E2_2168 at 2573.865 kb on + strand, within Pf6N2E2_2168 at 2573.865 kb on + strand, within Pf6N2E2_2168 at 2573.866 kb on - strand, within Pf6N2E2_2168 at 2573.866 kb on - strand, within Pf6N2E2_2168 at 2573.901 kb on + strand, within Pf6N2E2_2168 at 2573.902 kb on - strand, within Pf6N2E2_2168 at 2573.902 kb on - strand, within Pf6N2E2_2168 at 2573.902 kb on - strand, within Pf6N2E2_2168 at 2573.902 kb on - strand, within Pf6N2E2_2168 at 2573.966 kb on + strand at 2573.966 kb on + strand at 2573.966 kb on + strand at 2573.966 kb on + strand at 2573.966 kb on + strand at 2573.966 kb on + strand at 2573.967 kb on - strand at 2574.043 kb on + strand at 2574.043 kb on + strand at 2574.043 kb on + strand at 2574.044 kb on - strand at 2574.044 kb on - strand at 2574.044 kb on - strand at 2574.078 kb on - strand at 2574.078 kb on - strand at 2574.108 kb on + strand at 2574.109 kb on - strand at 2574.135 kb on + strand at 2574.136 kb on - strand at 2574.153 kb on - strand at 2574.162 kb on + strand at 2574.162 kb on + strand at 2574.162 kb on + strand at 2574.162 kb on + strand at 2574.162 kb on + strand at 2574.162 kb on + strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand at 2574.163 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 2,571,054 + -0.8 2,571,062 + -0.1 2,571,063 - +0.3 2,571,171 + +0.4 2,571,171 + -1.2 2,571,171 + +0.2 2,571,172 - +0.8 2,571,172 - +0.5 2,571,172 - -0.7 2,571,259 - Pf6N2E2_2166 0.17 -0.7 2,571,259 - Pf6N2E2_2166 0.17 -0.5 2,571,498 + Pf6N2E2_2166 0.42 -0.4 2,571,498 + Pf6N2E2_2166 0.42 -0.8 2,571,498 + Pf6N2E2_2166 0.42 -0.4 2,571,498 + Pf6N2E2_2166 0.42 -0.7 2,571,498 + Pf6N2E2_2166 0.42 -0.8 2,571,499 - Pf6N2E2_2166 0.42 -0.2 2,571,499 - Pf6N2E2_2166 0.42 -0.2 2,571,499 - Pf6N2E2_2166 0.42 +0.3 2,571,499 - Pf6N2E2_2166 0.42 -0.1 2,571,499 - Pf6N2E2_2166 0.42 -0.1 2,571,499 - Pf6N2E2_2166 0.42 -0.1 2,571,499 - Pf6N2E2_2166 0.42 +0.8 2,571,500 + Pf6N2E2_2166 0.42 +0.9 2,571,500 + Pf6N2E2_2166 0.42 +0.0 2,571,500 + Pf6N2E2_2166 0.42 +0.6 2,571,500 + Pf6N2E2_2166 0.42 +0.6 2,571,500 + Pf6N2E2_2166 0.42 -0.3 2,571,500 + Pf6N2E2_2166 0.42 +0.8 2,571,501 - Pf6N2E2_2166 0.42 +1.0 2,571,501 - Pf6N2E2_2166 0.42 +0.2 2,571,501 - Pf6N2E2_2166 0.42 -0.3 2,571,502 + Pf6N2E2_2166 0.43 -0.8 2,571,502 + Pf6N2E2_2166 0.43 -0.0 2,571,502 + Pf6N2E2_2166 0.43 -0.6 2,571,502 + Pf6N2E2_2166 0.43 +1.0 2,571,503 - Pf6N2E2_2166 0.43 -1.5 2,571,503 - Pf6N2E2_2166 0.43 -0.5 2,571,535 - Pf6N2E2_2166 0.46 +1.8 2,571,535 - Pf6N2E2_2166 0.46 +0.7 2,571,718 - Pf6N2E2_2166 0.65 -0.3 2,571,745 - Pf6N2E2_2166 0.68 +0.8 2,571,745 - Pf6N2E2_2166 0.68 +0.0 2,571,838 + Pf6N2E2_2166 0.78 -0.6 2,571,839 - Pf6N2E2_2166 0.78 +0.2 2,571,839 - Pf6N2E2_2166 0.78 -0.1 2,571,839 - Pf6N2E2_2166 0.78 +0.3 2,571,981 - -1.0 2,571,999 + -0.2 2,572,000 - +0.8 2,572,000 - +0.5 2,572,000 - +0.2 2,572,020 - -1.4 2,572,020 - -0.1 2,572,044 - -1.3 2,572,044 - +0.9 2,572,044 - +1.1 2,572,055 + -0.5 2,572,056 - +0.4 2,572,056 - +0.1 2,572,056 - +2.3 2,572,076 + +0.4 2,572,115 + +0.5 2,572,115 + +0.6 2,572,115 + -1.0 2,572,115 + +0.8 2,572,116 - +2.2 2,572,163 + -0.9 2,572,164 - +0.2 2,572,164 - +0.5 2,572,178 + Pf6N2E2_2167 0.10 -0.0 2,572,178 + Pf6N2E2_2167 0.10 +0.1 2,572,178 + Pf6N2E2_2167 0.10 +0.1 2,572,178 + Pf6N2E2_2167 0.10 -0.2 2,572,179 - Pf6N2E2_2167 0.10 -1.0 2,572,179 - Pf6N2E2_2167 0.10 -0.0 2,572,492 + Pf6N2E2_2167 0.35 -0.5 2,572,628 + Pf6N2E2_2167 0.45 +0.1 2,572,629 - Pf6N2E2_2167 0.45 -1.6 2,572,646 + Pf6N2E2_2167 0.47 +0.0 2,572,796 + Pf6N2E2_2167 0.58 +0.0 2,572,797 - Pf6N2E2_2167 0.59 -0.3 2,572,797 - Pf6N2E2_2167 0.59 +0.1 2,572,797 - Pf6N2E2_2167 0.59 -1.7 2,572,797 - Pf6N2E2_2167 0.59 -0.1 2,572,976 + Pf6N2E2_2167 0.73 +0.5 2,572,976 + Pf6N2E2_2167 0.73 +0.8 2,572,976 + Pf6N2E2_2167 0.73 +0.3 2,572,976 + Pf6N2E2_2167 0.73 +0.8 2,572,976 + Pf6N2E2_2167 0.73 -0.1 2,572,977 - Pf6N2E2_2167 0.73 +0.7 2,572,977 - Pf6N2E2_2167 0.73 +0.5 2,572,977 - Pf6N2E2_2167 0.73 -0.4 2,572,977 - Pf6N2E2_2167 0.73 -1.2 2,572,997 + Pf6N2E2_2167 0.74 -0.3 2,572,998 - Pf6N2E2_2167 0.74 -0.1 2,572,998 - Pf6N2E2_2167 0.74 +0.4 2,573,168 - Pf6N2E2_2167 0.88 +0.5 2,573,168 - Pf6N2E2_2167 0.88 +0.0 2,573,168 - Pf6N2E2_2167 0.88 +1.2 2,573,189 + Pf6N2E2_2167 0.89 +0.9 2,573,197 + Pf6N2E2_2167 0.90 +0.3 2,573,197 + Pf6N2E2_2167 0.90 -0.9 2,573,197 + Pf6N2E2_2167 0.90 -1.7 2,573,197 + Pf6N2E2_2167 0.90 -0.3 2,573,198 - Pf6N2E2_2167 0.90 +1.0 2,573,201 + +0.0 2,573,201 + -0.9 2,573,201 + -0.0 2,573,201 + -0.2 2,573,202 - -1.0 2,573,202 - +0.1 2,573,232 - -0.5 2,573,232 - -0.2 2,573,240 + +0.2 2,573,359 + -0.1 2,573,359 + +0.1 2,573,359 + -0.9 2,573,359 + -0.5 2,573,359 + -0.1 2,573,359 + +0.2 2,573,360 - +0.3 2,573,360 - -0.6 2,573,360 - -0.1 2,573,360 - -0.8 2,573,360 - -2.5 2,573,360 - +0.5 2,573,360 - +0.2 2,573,360 - +0.2 2,573,360 - +0.4 2,573,361 + -0.5 2,573,361 + -0.7 2,573,361 + -0.6 2,573,361 + +1.6 2,573,362 - +0.4 2,573,362 - -0.1 2,573,362 - -0.1 2,573,362 - -1.7 2,573,362 - +0.1 2,573,362 - -0.9 2,573,445 + Pf6N2E2_2168 0.15 -0.3 2,573,865 + Pf6N2E2_2168 0.81 +0.3 2,573,865 + Pf6N2E2_2168 0.81 -0.0 2,573,866 - Pf6N2E2_2168 0.81 +0.7 2,573,866 - Pf6N2E2_2168 0.81 +1.1 2,573,901 + Pf6N2E2_2168 0.86 -0.0 2,573,902 - Pf6N2E2_2168 0.86 +1.2 2,573,902 - Pf6N2E2_2168 0.86 -0.2 2,573,902 - Pf6N2E2_2168 0.86 +0.3 2,573,902 - Pf6N2E2_2168 0.86 +0.3 2,573,966 + -0.1 2,573,966 + +0.8 2,573,966 + -1.1 2,573,966 + -1.0 2,573,966 + +0.6 2,573,966 + -1.1 2,573,967 - -0.3 2,574,043 + +0.3 2,574,043 + +0.3 2,574,043 + -0.1 2,574,044 - +1.6 2,574,044 - -1.3 2,574,044 - -0.9 2,574,078 - +0.1 2,574,078 - +0.6 2,574,108 + +0.7 2,574,109 - +0.4 2,574,135 + -0.6 2,574,136 - -2.8 2,574,153 - -1.3 2,574,162 + +0.4 2,574,162 + -0.1 2,574,162 + +1.1 2,574,162 + -0.9 2,574,162 + +1.0 2,574,162 + +0.8 2,574,163 - +0.5 2,574,163 - +0.4 2,574,163 - +0.8 2,574,163 - +0.3 2,574,163 - -0.0 2,574,163 - -0.2 2,574,163 - +0.6 2,574,163 - -0.4 2,574,163 - +0.8 2,574,163 - -0.8 2,574,163 - -0.5 2,574,163 - -0.3 2,574,163 - -0.4
Or see this region's nucleotide sequence