Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1954 and Pf6N2E2_1955 are separated by 477 nucleotides Pf6N2E2_1955 and Pf6N2E2_1956 are separated by 49 nucleotides
Pf6N2E2_1954 - Transcriptional regulator, LysR family, at 2,318,595 to 2,319,482
_1954
Pf6N2E2_1955 - daunorubicin C-13 ketoreductase XF1741, at 2,319,960 to 2,320,559
_1955
Pf6N2E2_1956 - Outer membrane protein romA, at 2,320,609 to 2,321,745
_1956
Position (kb)
2319
2320
2321 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2319.091 kb on + strand, within Pf6N2E2_1954 at 2319.189 kb on - strand, within Pf6N2E2_1954 at 2319.191 kb on + strand, within Pf6N2E2_1954 at 2319.192 kb on - strand, within Pf6N2E2_1954 at 2319.279 kb on + strand, within Pf6N2E2_1954 at 2319.297 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.302 kb on + strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.303 kb on - strand, within Pf6N2E2_1954 at 2319.309 kb on + strand, within Pf6N2E2_1954 at 2319.310 kb on - strand, within Pf6N2E2_1954 at 2319.330 kb on - strand, within Pf6N2E2_1954 at 2319.399 kb on + strand at 2319.399 kb on + strand at 2319.403 kb on - strand at 2319.453 kb on + strand at 2319.453 kb on + strand at 2319.511 kb on - strand at 2319.521 kb on - strand at 2319.569 kb on + strand at 2319.570 kb on - strand at 2319.570 kb on - strand at 2319.570 kb on - strand at 2319.574 kb on + strand at 2319.575 kb on - strand at 2319.601 kb on + strand at 2319.601 kb on + strand at 2319.603 kb on + strand at 2319.603 kb on + strand at 2319.603 kb on + strand at 2319.678 kb on - strand at 2319.678 kb on - strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.818 kb on + strand at 2319.819 kb on - strand at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.099 kb on + strand, within Pf6N2E2_1955 at 2320.100 kb on - strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.122 kb on + strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.123 kb on - strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.124 kb on + strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.125 kb on - strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.164 kb on + strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.165 kb on - strand, within Pf6N2E2_1955 at 2320.185 kb on - strand, within Pf6N2E2_1955 at 2320.246 kb on - strand, within Pf6N2E2_1955 at 2320.316 kb on + strand, within Pf6N2E2_1955 at 2320.317 kb on - strand, within Pf6N2E2_1955 at 2320.573 kb on - strand at 2320.576 kb on + strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.577 kb on - strand at 2320.590 kb on - strand at 2320.635 kb on - strand at 2320.657 kb on + strand at 2320.658 kb on - strand at 2320.661 kb on + strand at 2320.661 kb on + strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.662 kb on - strand at 2320.891 kb on - strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.901 kb on + strand, within Pf6N2E2_1956 at 2320.902 kb on - strand, within Pf6N2E2_1956 at 2320.928 kb on - strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.982 kb on - strand, within Pf6N2E2_1956 at 2321.049 kb on - strand, within Pf6N2E2_1956 at 2321.087 kb on - strand, within Pf6N2E2_1956 at 2321.102 kb on - strand, within Pf6N2E2_1956 at 2321.159 kb on + strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.419 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 2,319,091 + Pf6N2E2_1954 0.56 -1.2 2,319,189 - Pf6N2E2_1954 0.67 -1.6 2,319,191 + Pf6N2E2_1954 0.67 -1.2 2,319,192 - Pf6N2E2_1954 0.67 -1.8 2,319,279 + Pf6N2E2_1954 0.77 +1.0 2,319,297 + Pf6N2E2_1954 0.79 -0.5 2,319,302 + Pf6N2E2_1954 0.80 +0.1 2,319,302 + Pf6N2E2_1954 0.80 -0.4 2,319,303 - Pf6N2E2_1954 0.80 -0.2 2,319,303 - Pf6N2E2_1954 0.80 -0.1 2,319,303 - Pf6N2E2_1954 0.80 +1.8 2,319,309 + Pf6N2E2_1954 0.80 -0.3 2,319,310 - Pf6N2E2_1954 0.81 +0.8 2,319,330 - Pf6N2E2_1954 0.83 -0.6 2,319,399 + +0.7 2,319,399 + -0.4 2,319,403 - +0.8 2,319,453 + -0.4 2,319,453 + +0.3 2,319,511 - -0.7 2,319,521 - +0.1 2,319,569 + -0.1 2,319,570 - -1.5 2,319,570 - +0.9 2,319,570 - -0.4 2,319,574 + -0.8 2,319,575 - -0.2 2,319,601 + +0.5 2,319,601 + +0.1 2,319,603 + +0.4 2,319,603 + -0.8 2,319,603 + -0.3 2,319,678 - -1.0 2,319,678 - +1.1 2,319,818 + -1.0 2,319,818 + -0.7 2,319,818 + +0.1 2,319,819 - +0.8 2,320,099 + Pf6N2E2_1955 0.23 +0.3 2,320,099 + Pf6N2E2_1955 0.23 +0.3 2,320,100 - Pf6N2E2_1955 0.23 -0.2 2,320,122 + Pf6N2E2_1955 0.27 -0.2 2,320,122 + Pf6N2E2_1955 0.27 +1.0 2,320,123 - Pf6N2E2_1955 0.27 -0.5 2,320,123 - Pf6N2E2_1955 0.27 +1.4 2,320,124 + Pf6N2E2_1955 0.27 +0.4 2,320,124 + Pf6N2E2_1955 0.27 -0.1 2,320,124 + Pf6N2E2_1955 0.27 +0.7 2,320,124 + Pf6N2E2_1955 0.27 -0.2 2,320,124 + Pf6N2E2_1955 0.27 -0.2 2,320,124 + Pf6N2E2_1955 0.27 -0.1 2,320,124 + Pf6N2E2_1955 0.27 +1.0 2,320,125 - Pf6N2E2_1955 0.28 +1.6 2,320,125 - Pf6N2E2_1955 0.28 -0.3 2,320,125 - Pf6N2E2_1955 0.28 +0.6 2,320,125 - Pf6N2E2_1955 0.28 +0.9 2,320,125 - Pf6N2E2_1955 0.28 -0.2 2,320,164 + Pf6N2E2_1955 0.34 -0.6 2,320,164 + Pf6N2E2_1955 0.34 +0.5 2,320,165 - Pf6N2E2_1955 0.34 +0.9 2,320,165 - Pf6N2E2_1955 0.34 -0.4 2,320,165 - Pf6N2E2_1955 0.34 +0.1 2,320,185 - Pf6N2E2_1955 0.38 +0.4 2,320,246 - Pf6N2E2_1955 0.48 +1.2 2,320,316 + Pf6N2E2_1955 0.59 +1.4 2,320,317 - Pf6N2E2_1955 0.59 -0.6 2,320,573 - -0.1 2,320,576 + -1.2 2,320,577 - -0.2 2,320,577 - -0.3 2,320,577 - +0.3 2,320,577 - +1.1 2,320,577 - +1.5 2,320,590 - -0.3 2,320,635 - -0.4 2,320,657 + -0.8 2,320,658 - +1.8 2,320,661 + +0.1 2,320,661 + +0.2 2,320,662 - -0.0 2,320,662 - -0.6 2,320,662 - -1.0 2,320,662 - +0.4 2,320,662 - -0.4 2,320,891 - Pf6N2E2_1956 0.25 -0.8 2,320,899 + Pf6N2E2_1956 0.26 -0.8 2,320,899 + Pf6N2E2_1956 0.26 -0.3 2,320,900 - Pf6N2E2_1956 0.26 +0.6 2,320,900 - Pf6N2E2_1956 0.26 +0.1 2,320,901 + Pf6N2E2_1956 0.26 -0.2 2,320,902 - Pf6N2E2_1956 0.26 +0.1 2,320,928 - Pf6N2E2_1956 0.28 +1.4 2,320,981 + Pf6N2E2_1956 0.33 -1.2 2,320,981 + Pf6N2E2_1956 0.33 -0.4 2,320,982 - Pf6N2E2_1956 0.33 -1.8 2,321,049 - Pf6N2E2_1956 0.39 -1.2 2,321,087 - Pf6N2E2_1956 0.42 +0.9 2,321,102 - Pf6N2E2_1956 0.43 -0.1 2,321,159 + Pf6N2E2_1956 0.48 +0.2 2,321,160 - Pf6N2E2_1956 0.48 -0.4 2,321,160 - Pf6N2E2_1956 0.48 +1.4 2,321,276 - Pf6N2E2_1956 0.59 +0.8 2,321,276 - Pf6N2E2_1956 0.59 +0.2 2,321,419 + Pf6N2E2_1956 0.71 -0.2 2,321,443 + Pf6N2E2_1956 0.73 -0.7 2,321,443 + Pf6N2E2_1956 0.73 -0.6 2,321,443 + Pf6N2E2_1956 0.73 -0.7
Or see this region's nucleotide sequence