Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1763 and Pf6N2E2_1764 are separated by 111 nucleotides Pf6N2E2_1764 and Pf6N2E2_1765 are separated by 12 nucleotides
Pf6N2E2_1763 - Cyclohexanone monooxygenase (EC 1.14.13.22), at 2,082,232 to 2,084,154
_1763
Pf6N2E2_1764 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,084,266 to 2,085,774
_1764
Pf6N2E2_1765 - hypothetical protein, at 2,085,787 to 2,087,091
_1765
Position (kb)
2084
2085
2086 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2083.334 kb on - strand, within Pf6N2E2_1763 at 2083.520 kb on + strand, within Pf6N2E2_1763 at 2083.521 kb on - strand, within Pf6N2E2_1763 at 2083.602 kb on - strand, within Pf6N2E2_1763 at 2083.638 kb on + strand, within Pf6N2E2_1763 at 2083.648 kb on + strand, within Pf6N2E2_1763 at 2083.648 kb on + strand, within Pf6N2E2_1763 at 2083.648 kb on + strand, within Pf6N2E2_1763 at 2083.690 kb on + strand, within Pf6N2E2_1763 at 2083.795 kb on + strand, within Pf6N2E2_1763 at 2083.840 kb on + strand, within Pf6N2E2_1763 at 2083.840 kb on + strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.841 kb on - strand, within Pf6N2E2_1763 at 2083.906 kb on + strand, within Pf6N2E2_1763 at 2083.928 kb on + strand, within Pf6N2E2_1763 at 2083.929 kb on - strand, within Pf6N2E2_1763 at 2083.984 kb on + strand at 2083.985 kb on - strand at 2084.140 kb on + strand at 2084.140 kb on + strand at 2084.179 kb on + strand at 2084.179 kb on + strand at 2084.183 kb on + strand at 2084.183 kb on + strand at 2084.183 kb on + strand at 2084.184 kb on - strand at 2084.195 kb on + strand at 2084.224 kb on + strand at 2084.224 kb on + strand at 2084.224 kb on + strand at 2084.225 kb on - strand at 2084.225 kb on - strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.347 kb on + strand at 2084.348 kb on - strand at 2084.392 kb on - strand at 2084.462 kb on + strand, within Pf6N2E2_1764 at 2084.463 kb on - strand, within Pf6N2E2_1764 at 2084.541 kb on + strand, within Pf6N2E2_1764 at 2084.665 kb on + strand, within Pf6N2E2_1764 at 2084.668 kb on + strand, within Pf6N2E2_1764 at 2084.669 kb on - strand, within Pf6N2E2_1764 at 2084.747 kb on - strand, within Pf6N2E2_1764 at 2084.749 kb on - strand, within Pf6N2E2_1764 at 2084.995 kb on - strand, within Pf6N2E2_1764 at 2085.049 kb on + strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.050 kb on - strand, within Pf6N2E2_1764 at 2085.159 kb on + strand, within Pf6N2E2_1764 at 2085.160 kb on - strand, within Pf6N2E2_1764 at 2085.160 kb on - strand, within Pf6N2E2_1764 at 2085.240 kb on - strand, within Pf6N2E2_1764 at 2085.352 kb on + strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.353 kb on - strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.421 kb on + strand, within Pf6N2E2_1764 at 2085.482 kb on + strand, within Pf6N2E2_1764 at 2085.482 kb on + strand, within Pf6N2E2_1764 at 2085.640 kb on - strand at 2085.675 kb on + strand at 2085.675 kb on + strand at 2085.902 kb on - strand at 2085.902 kb on - strand at 2086.210 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.236 kb on + strand, within Pf6N2E2_1765 at 2086.237 kb on - strand, within Pf6N2E2_1765 at 2086.312 kb on + strand, within Pf6N2E2_1765 at 2086.346 kb on + strand, within Pf6N2E2_1765 at 2086.347 kb on - strand, within Pf6N2E2_1765 at 2086.571 kb on + strand, within Pf6N2E2_1765 at 2086.579 kb on + strand, within Pf6N2E2_1765 at 2086.628 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.637 kb on - strand, within Pf6N2E2_1765 at 2086.639 kb on - strand, within Pf6N2E2_1765
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 2,083,334 - Pf6N2E2_1763 0.57 +0.5 2,083,520 + Pf6N2E2_1763 0.67 -0.8 2,083,521 - Pf6N2E2_1763 0.67 +0.1 2,083,602 - Pf6N2E2_1763 0.71 -0.2 2,083,638 + Pf6N2E2_1763 0.73 +0.0 2,083,648 + Pf6N2E2_1763 0.74 -0.4 2,083,648 + Pf6N2E2_1763 0.74 +1.3 2,083,648 + Pf6N2E2_1763 0.74 +1.0 2,083,690 + Pf6N2E2_1763 0.76 -1.2 2,083,795 + Pf6N2E2_1763 0.81 -0.4 2,083,840 + Pf6N2E2_1763 0.84 -0.6 2,083,840 + Pf6N2E2_1763 0.84 +0.2 2,083,841 - Pf6N2E2_1763 0.84 +1.8 2,083,841 - Pf6N2E2_1763 0.84 +0.5 2,083,841 - Pf6N2E2_1763 0.84 +0.8 2,083,841 - Pf6N2E2_1763 0.84 +1.0 2,083,841 - Pf6N2E2_1763 0.84 +0.1 2,083,841 - Pf6N2E2_1763 0.84 -0.1 2,083,841 - Pf6N2E2_1763 0.84 -0.7 2,083,906 + Pf6N2E2_1763 0.87 -0.8 2,083,928 + Pf6N2E2_1763 0.88 +0.6 2,083,929 - Pf6N2E2_1763 0.88 +1.2 2,083,984 + -1.9 2,083,985 - +0.3 2,084,140 + +0.4 2,084,140 + -0.2 2,084,179 + -0.5 2,084,179 + -1.8 2,084,183 + +1.7 2,084,183 + -0.0 2,084,183 + -0.3 2,084,184 - -0.4 2,084,195 + -1.8 2,084,224 + -0.5 2,084,224 + +0.5 2,084,224 + +2.3 2,084,225 - -0.4 2,084,225 - -1.4 2,084,347 + -0.3 2,084,347 + -1.1 2,084,347 + -0.1 2,084,347 + +0.8 2,084,347 + +0.4 2,084,347 + +0.2 2,084,347 + +0.8 2,084,348 - -1.8 2,084,392 - -1.0 2,084,462 + Pf6N2E2_1764 0.13 +0.3 2,084,463 - Pf6N2E2_1764 0.13 -0.5 2,084,541 + Pf6N2E2_1764 0.18 +1.6 2,084,665 + Pf6N2E2_1764 0.26 +0.8 2,084,668 + Pf6N2E2_1764 0.27 -0.3 2,084,669 - Pf6N2E2_1764 0.27 -1.3 2,084,747 - Pf6N2E2_1764 0.32 -0.2 2,084,749 - Pf6N2E2_1764 0.32 +0.6 2,084,995 - Pf6N2E2_1764 0.48 +0.2 2,085,049 + Pf6N2E2_1764 0.52 +2.2 2,085,050 - Pf6N2E2_1764 0.52 -0.8 2,085,050 - Pf6N2E2_1764 0.52 -1.9 2,085,050 - Pf6N2E2_1764 0.52 -1.5 2,085,159 + Pf6N2E2_1764 0.59 -0.8 2,085,160 - Pf6N2E2_1764 0.59 +0.3 2,085,160 - Pf6N2E2_1764 0.59 +0.5 2,085,240 - Pf6N2E2_1764 0.65 +0.3 2,085,352 + Pf6N2E2_1764 0.72 +0.0 2,085,353 - Pf6N2E2_1764 0.72 -0.6 2,085,353 - Pf6N2E2_1764 0.72 -0.2 2,085,353 - Pf6N2E2_1764 0.72 +1.1 2,085,353 - Pf6N2E2_1764 0.72 -2.0 2,085,421 + Pf6N2E2_1764 0.77 +0.2 2,085,421 + Pf6N2E2_1764 0.77 -3.1 2,085,421 + Pf6N2E2_1764 0.77 +0.2 2,085,482 + Pf6N2E2_1764 0.81 -0.2 2,085,482 + Pf6N2E2_1764 0.81 +0.3 2,085,640 - -0.7 2,085,675 + +0.3 2,085,675 + +0.2 2,085,902 - +0.6 2,085,902 - +2.3 2,086,210 + Pf6N2E2_1765 0.32 -0.6 2,086,236 + Pf6N2E2_1765 0.34 +0.2 2,086,236 + Pf6N2E2_1765 0.34 +0.4 2,086,237 - Pf6N2E2_1765 0.34 -0.2 2,086,312 + Pf6N2E2_1765 0.40 -0.5 2,086,346 + Pf6N2E2_1765 0.43 -1.1 2,086,347 - Pf6N2E2_1765 0.43 +0.7 2,086,571 + Pf6N2E2_1765 0.60 -0.5 2,086,579 + Pf6N2E2_1765 0.61 -0.7 2,086,628 - Pf6N2E2_1765 0.64 +0.2 2,086,637 - Pf6N2E2_1765 0.65 +0.7 2,086,637 - Pf6N2E2_1765 0.65 +0.3 2,086,639 - Pf6N2E2_1765 0.65 -1.1
Or see this region's nucleotide sequence