Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_167 and Pf6N2E2_168 are separated by 48 nucleotides Pf6N2E2_168 and Pf6N2E2_169 are separated by 128 nucleotides Pf6N2E2_169 and Pf6N2E2_170 are separated by 162 nucleotides Pf6N2E2_170 and Pf6N2E2_171 are separated by 68 nucleotides
Pf6N2E2_167 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 220,216 to 221,244
_167
Pf6N2E2_168 - hypothetical protein, at 221,293 to 221,490
_168
Pf6N2E2_169 - FIG00955358: hypothetical protein, at 221,619 to 221,924
_169
Pf6N2E2_170 - lipoprotein, putative, at 222,087 to 222,401
_170
Pf6N2E2_171 - Cold shock protein CspG, at 222,470 to 222,682
_171
Position (kb) 221
222 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 220.624 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.785 kb on + strand, within Pf6N2E2_167 at 220.867 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.978 kb on + strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 220.979 kb on - strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.002 kb on + strand, within Pf6N2E2_167 at 221.144 kb on - strand at 221.144 kb on - strand at 221.255 kb on + strand at 221.255 kb on + strand at 221.256 kb on - strand at 221.256 kb on - strand at 221.257 kb on + strand at 221.258 kb on - strand at 221.258 kb on - strand at 221.389 kb on + strand, within Pf6N2E2_168 at 221.389 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.429 kb on + strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.430 kb on - strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.436 kb on + strand, within Pf6N2E2_168 at 221.437 kb on - strand, within Pf6N2E2_168 at 221.493 kb on + strand at 221.493 kb on + strand at 221.494 kb on - strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.517 kb on + strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.518 kb on - strand at 221.527 kb on + strand at 221.528 kb on - strand at 221.561 kb on + strand at 221.562 kb on - strand at 221.562 kb on - strand at 221.702 kb on + strand, within Pf6N2E2_169 at 221.703 kb on - strand, within Pf6N2E2_169 at 221.736 kb on + strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.737 kb on - strand, within Pf6N2E2_169 at 221.817 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.865 kb on + strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.866 kb on - strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.882 kb on + strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.883 kb on - strand, within Pf6N2E2_169 at 221.907 kb on + strand at 221.907 kb on + strand at 221.920 kb on - strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.063 kb on + strand at 222.064 kb on - strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.113 kb on + strand at 222.114 kb on - strand at 222.114 kb on - strand at 222.359 kb on + strand, within Pf6N2E2_170 at 222.360 kb on - strand, within Pf6N2E2_170 at 222.455 kb on + strand at 222.472 kb on - strand at 222.588 kb on - strand, within Pf6N2E2_171 at 222.687 kb on + strand at 222.703 kb on - strand at 222.703 kb on - strand at 222.704 kb on + strand at 222.704 kb on + strand at 222.705 kb on - strand at 222.715 kb on + strand at 222.715 kb on + strand at 222.716 kb on - strand at 222.716 kb on - strand at 222.755 kb on + strand at 222.903 kb on - strand at 222.903 kb on - strand at 222.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 220,624 + Pf6N2E2_167 0.40 +0.4 220,785 + Pf6N2E2_167 0.55 +0.0 220,785 + Pf6N2E2_167 0.55 +1.7 220,867 + Pf6N2E2_167 0.63 -2.6 220,978 + Pf6N2E2_167 0.74 +0.5 220,978 + Pf6N2E2_167 0.74 +0.5 220,979 - Pf6N2E2_167 0.74 +0.4 220,979 - Pf6N2E2_167 0.74 -1.8 220,979 - Pf6N2E2_167 0.74 -0.7 220,979 - Pf6N2E2_167 0.74 -0.4 220,979 - Pf6N2E2_167 0.74 -3.2 220,979 - Pf6N2E2_167 0.74 +0.6 221,002 + Pf6N2E2_167 0.76 +0.8 221,002 + Pf6N2E2_167 0.76 -0.8 221,144 - +1.0 221,144 - -0.6 221,255 + -1.2 221,255 + +0.3 221,256 - +1.1 221,256 - +0.7 221,257 + -0.6 221,258 - +0.1 221,258 - +0.8 221,389 + Pf6N2E2_168 0.48 +1.4 221,389 + Pf6N2E2_168 0.48 +1.2 221,429 + Pf6N2E2_168 0.69 +0.9 221,429 + Pf6N2E2_168 0.69 +0.1 221,429 + Pf6N2E2_168 0.69 +0.9 221,430 - Pf6N2E2_168 0.69 +0.3 221,430 - Pf6N2E2_168 0.69 +0.1 221,430 - Pf6N2E2_168 0.69 -0.1 221,436 + Pf6N2E2_168 0.72 +0.0 221,436 + Pf6N2E2_168 0.72 +0.9 221,437 - Pf6N2E2_168 0.73 -1.1 221,493 + +0.2 221,493 + +0.8 221,494 - +0.4 221,517 + +0.7 221,517 + +1.0 221,517 + +1.3 221,518 - +0.5 221,518 - -0.2 221,518 - -0.0 221,518 - -0.6 221,527 + +0.2 221,528 - -0.5 221,561 + -0.2 221,562 - +0.4 221,562 - -0.7 221,702 + Pf6N2E2_169 0.27 +0.4 221,703 - Pf6N2E2_169 0.27 +0.0 221,736 + Pf6N2E2_169 0.38 +0.6 221,737 - Pf6N2E2_169 0.39 -0.0 221,737 - Pf6N2E2_169 0.39 -1.4 221,737 - Pf6N2E2_169 0.39 -0.4 221,737 - Pf6N2E2_169 0.39 +0.3 221,817 + Pf6N2E2_169 0.65 +0.1 221,865 + Pf6N2E2_169 0.80 +0.9 221,865 + Pf6N2E2_169 0.80 +0.5 221,865 + Pf6N2E2_169 0.80 +1.3 221,865 + Pf6N2E2_169 0.80 +0.1 221,865 + Pf6N2E2_169 0.80 +0.4 221,866 - Pf6N2E2_169 0.81 -0.1 221,866 - Pf6N2E2_169 0.81 -1.2 221,866 - Pf6N2E2_169 0.81 +0.8 221,866 - Pf6N2E2_169 0.81 +0.6 221,866 - Pf6N2E2_169 0.81 +0.1 221,866 - Pf6N2E2_169 0.81 +1.8 221,866 - Pf6N2E2_169 0.81 +0.4 221,866 - Pf6N2E2_169 0.81 -0.1 221,882 + Pf6N2E2_169 0.86 -0.9 221,882 + Pf6N2E2_169 0.86 +1.4 221,882 + Pf6N2E2_169 0.86 -0.5 221,883 - Pf6N2E2_169 0.86 -0.2 221,883 - Pf6N2E2_169 0.86 +1.0 221,907 + +0.7 221,907 + +0.0 221,920 - +1.0 222,063 + +0.4 222,063 + +1.0 222,063 + +0.0 222,064 - +0.3 222,113 + +1.1 222,113 + +0.3 222,113 + +0.2 222,114 - -0.7 222,114 - +0.0 222,359 + Pf6N2E2_170 0.86 +1.2 222,360 - Pf6N2E2_170 0.87 +0.2 222,455 + -1.4 222,472 - -0.2 222,588 - Pf6N2E2_171 0.55 +0.2 222,687 + -1.5 222,703 - +0.2 222,703 - -0.3 222,704 + +0.6 222,704 + +0.4 222,705 - +0.5 222,715 + +1.1 222,715 + -0.8 222,716 - +1.0 222,716 - +0.9 222,755 + +1.2 222,903 - +0.7 222,903 - +0.9 222,903 - +0.6
Or see this region's nucleotide sequence