Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1443 and Pf6N2E2_1444 are separated by 92 nucleotides Pf6N2E2_1444 and Pf6N2E2_1445 are separated by 133 nucleotides Pf6N2E2_1445 and Pf6N2E2_1446 are separated by 238 nucleotides
Pf6N2E2_1443 - putative aldo/keto reductase, at 1,693,084 to 1,694,091
_1443
Pf6N2E2_1444 - Transcriptional regulator, LysR family, at 1,694,184 to 1,695,086
_1444
Pf6N2E2_1445 - Glutathione S-transferase, at 1,695,220 to 1,695,831
_1445
Pf6N2E2_1446 - Enoyl-[acyl-carrier-protein] reductase [NADPH] (EC 1.3.1.10), at 1,696,070 to 1,696,822
_1446
Position (kb)
1694
1695
1696 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.452 kb on - strand, within Pf6N2E2_1443 at 1693.527 kb on - strand, within Pf6N2E2_1443 at 1693.573 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.911 kb on - strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1693.955 kb on + strand, within Pf6N2E2_1443 at 1694.091 kb on + strand at 1694.092 kb on - strand at 1694.386 kb on + strand, within Pf6N2E2_1444 at 1694.706 kb on + strand, within Pf6N2E2_1444 at 1694.706 kb on + strand, within Pf6N2E2_1444 at 1694.707 kb on - strand, within Pf6N2E2_1444 at 1694.805 kb on + strand, within Pf6N2E2_1444 at 1694.805 kb on + strand, within Pf6N2E2_1444 at 1694.806 kb on - strand, within Pf6N2E2_1444 at 1695.006 kb on + strand at 1695.007 kb on - strand at 1695.007 kb on - strand at 1695.009 kb on + strand at 1695.010 kb on - strand at 1695.133 kb on + strand at 1695.133 kb on + strand at 1695.133 kb on + strand at 1695.134 kb on - strand at 1695.417 kb on - strand, within Pf6N2E2_1445 at 1695.470 kb on - strand, within Pf6N2E2_1445 at 1695.617 kb on - strand, within Pf6N2E2_1445 at 1695.691 kb on + strand, within Pf6N2E2_1445 at 1695.785 kb on - strand at 1695.796 kb on + strand at 1695.796 kb on + strand at 1695.797 kb on - strand at 1695.847 kb on + strand at 1695.860 kb on - strand at 1695.871 kb on + strand at 1695.879 kb on + strand at 1695.879 kb on + strand at 1695.879 kb on + strand at 1695.880 kb on - strand at 1695.898 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.916 kb on + strand at 1695.917 kb on - strand at 1695.920 kb on - strand at 1695.920 kb on - strand at 1695.991 kb on - strand at 1695.991 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 1,693,452 - Pf6N2E2_1443 0.37 -0.4 1,693,452 - Pf6N2E2_1443 0.37 +0.1 1,693,527 - Pf6N2E2_1443 0.44 +1.7 1,693,573 - Pf6N2E2_1443 0.49 -0.8 1,693,911 - Pf6N2E2_1443 0.82 -0.2 1,693,911 - Pf6N2E2_1443 0.82 +1.6 1,693,955 + Pf6N2E2_1443 0.86 +0.7 1,693,955 + Pf6N2E2_1443 0.86 +0.1 1,693,955 + Pf6N2E2_1443 0.86 +0.7 1,694,091 + +1.3 1,694,092 - -0.9 1,694,386 + Pf6N2E2_1444 0.22 -0.3 1,694,706 + Pf6N2E2_1444 0.58 -1.5 1,694,706 + Pf6N2E2_1444 0.58 -2.9 1,694,707 - Pf6N2E2_1444 0.58 -1.0 1,694,805 + Pf6N2E2_1444 0.69 -0.5 1,694,805 + Pf6N2E2_1444 0.69 +0.2 1,694,806 - Pf6N2E2_1444 0.69 +0.4 1,695,006 + +0.4 1,695,007 - -0.7 1,695,007 - +0.1 1,695,009 + -0.6 1,695,010 - +1.4 1,695,133 + -1.5 1,695,133 + -0.3 1,695,133 + -0.8 1,695,134 - +0.3 1,695,417 - Pf6N2E2_1445 0.32 -0.2 1,695,470 - Pf6N2E2_1445 0.41 -0.8 1,695,617 - Pf6N2E2_1445 0.65 +1.7 1,695,691 + Pf6N2E2_1445 0.77 -0.7 1,695,785 - -0.3 1,695,796 + +1.9 1,695,796 + +0.9 1,695,797 - +0.7 1,695,847 + -0.4 1,695,860 - +0.5 1,695,871 + -1.2 1,695,879 + -1.3 1,695,879 + +0.5 1,695,879 + +1.7 1,695,880 - -0.2 1,695,898 + -0.2 1,695,916 + +0.8 1,695,916 + -0.5 1,695,916 + +0.7 1,695,916 + +2.2 1,695,916 + +1.5 1,695,917 - +2.0 1,695,920 - -2.5 1,695,920 - -1.1 1,695,991 - +1.1 1,695,991 - -0.4
Or see this region's nucleotide sequence