Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1160 and Pf6N2E2_1161 are separated by 276 nucleotides Pf6N2E2_1161 and Pf6N2E2_1162 are separated by 63 nucleotides Pf6N2E2_1162 and Pf6N2E2_1163 are separated by 155 nucleotides
Pf6N2E2_1160 - Tricarboxylate porin OpdH, at 1,357,747 to 1,358,985
_1160
Pf6N2E2_1161 - FIG00961921: hypothetical protein, at 1,359,262 to 1,359,942
_1161
Pf6N2E2_1162 - Transmembrane amino acid efflux protein, at 1,360,006 to 1,360,635
_1162
Pf6N2E2_1163 - hypothetical protein, at 1,360,791 to 1,362,170
_1163
Position (kb)
1359
1360 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1358.411 kb on - strand, within Pf6N2E2_1160 at 1358.412 kb on + strand, within Pf6N2E2_1160 at 1358.413 kb on - strand, within Pf6N2E2_1160 at 1358.413 kb on - strand, within Pf6N2E2_1160 at 1358.431 kb on + strand, within Pf6N2E2_1160 at 1358.431 kb on + strand, within Pf6N2E2_1160 at 1358.431 kb on + strand, within Pf6N2E2_1160 at 1358.431 kb on + strand, within Pf6N2E2_1160 at 1358.443 kb on + strand, within Pf6N2E2_1160 at 1358.444 kb on - strand, within Pf6N2E2_1160 at 1358.575 kb on + strand, within Pf6N2E2_1160 at 1358.576 kb on - strand, within Pf6N2E2_1160 at 1358.576 kb on - strand, within Pf6N2E2_1160 at 1358.576 kb on - strand, within Pf6N2E2_1160 at 1358.576 kb on - strand, within Pf6N2E2_1160 at 1358.576 kb on - strand, within Pf6N2E2_1160 at 1358.642 kb on - strand, within Pf6N2E2_1160 at 1358.663 kb on - strand, within Pf6N2E2_1160 at 1358.728 kb on + strand, within Pf6N2E2_1160 at 1358.729 kb on - strand, within Pf6N2E2_1160 at 1358.729 kb on - strand, within Pf6N2E2_1160 at 1358.735 kb on - strand, within Pf6N2E2_1160 at 1358.736 kb on + strand, within Pf6N2E2_1160 at 1358.737 kb on - strand, within Pf6N2E2_1160 at 1358.782 kb on + strand, within Pf6N2E2_1160 at 1358.782 kb on + strand, within Pf6N2E2_1160 at 1358.783 kb on - strand, within Pf6N2E2_1160 at 1358.783 kb on - strand, within Pf6N2E2_1160 at 1358.857 kb on + strand, within Pf6N2E2_1160 at 1358.858 kb on - strand, within Pf6N2E2_1160 at 1358.858 kb on - strand, within Pf6N2E2_1160 at 1358.858 kb on - strand, within Pf6N2E2_1160 at 1358.965 kb on + strand at 1359.058 kb on + strand at 1359.058 kb on + strand at 1359.100 kb on + strand at 1359.100 kb on + strand at 1359.101 kb on - strand at 1359.158 kb on + strand at 1359.191 kb on + strand at 1359.191 kb on + strand at 1359.191 kb on + strand at 1359.404 kb on + strand, within Pf6N2E2_1161 at 1359.404 kb on + strand, within Pf6N2E2_1161 at 1359.414 kb on + strand, within Pf6N2E2_1161 at 1359.414 kb on + strand, within Pf6N2E2_1161 at 1359.415 kb on - strand, within Pf6N2E2_1161 at 1359.415 kb on - strand, within Pf6N2E2_1161 at 1359.650 kb on + strand, within Pf6N2E2_1161 at 1359.651 kb on - strand, within Pf6N2E2_1161 at 1359.651 kb on - strand, within Pf6N2E2_1161 at 1359.824 kb on - strand, within Pf6N2E2_1161 at 1359.824 kb on - strand, within Pf6N2E2_1161 at 1359.974 kb on + strand at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.071 kb on - strand, within Pf6N2E2_1162 at 1360.515 kb on + strand, within Pf6N2E2_1162 at 1360.613 kb on + strand at 1360.613 kb on + strand at 1360.614 kb on - strand at 1360.891 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 1,358,411 - Pf6N2E2_1160 0.54 +0.7 1,358,412 + Pf6N2E2_1160 0.54 +0.6 1,358,413 - Pf6N2E2_1160 0.54 -0.4 1,358,413 - Pf6N2E2_1160 0.54 +1.2 1,358,431 + Pf6N2E2_1160 0.55 -0.7 1,358,431 + Pf6N2E2_1160 0.55 +2.0 1,358,431 + Pf6N2E2_1160 0.55 +2.0 1,358,431 + Pf6N2E2_1160 0.55 +3.0 1,358,443 + Pf6N2E2_1160 0.56 +0.6 1,358,444 - Pf6N2E2_1160 0.56 -1.9 1,358,575 + Pf6N2E2_1160 0.67 +1.6 1,358,576 - Pf6N2E2_1160 0.67 +0.6 1,358,576 - Pf6N2E2_1160 0.67 -0.8 1,358,576 - Pf6N2E2_1160 0.67 -1.7 1,358,576 - Pf6N2E2_1160 0.67 +0.5 1,358,576 - Pf6N2E2_1160 0.67 -0.9 1,358,642 - Pf6N2E2_1160 0.72 +0.1 1,358,663 - Pf6N2E2_1160 0.74 +0.7 1,358,728 + Pf6N2E2_1160 0.79 +1.0 1,358,729 - Pf6N2E2_1160 0.79 +0.1 1,358,729 - Pf6N2E2_1160 0.79 +0.4 1,358,735 - Pf6N2E2_1160 0.80 -0.5 1,358,736 + Pf6N2E2_1160 0.80 +0.9 1,358,737 - Pf6N2E2_1160 0.80 -0.1 1,358,782 + Pf6N2E2_1160 0.84 +0.1 1,358,782 + Pf6N2E2_1160 0.84 +0.8 1,358,783 - Pf6N2E2_1160 0.84 -0.0 1,358,783 - Pf6N2E2_1160 0.84 -0.9 1,358,857 + Pf6N2E2_1160 0.90 +0.2 1,358,858 - Pf6N2E2_1160 0.90 -1.7 1,358,858 - Pf6N2E2_1160 0.90 -0.5 1,358,858 - Pf6N2E2_1160 0.90 -0.7 1,358,965 + -1.4 1,359,058 + -1.0 1,359,058 + -0.2 1,359,100 + +0.2 1,359,100 + +1.6 1,359,101 - -0.4 1,359,158 + -0.5 1,359,191 + +0.2 1,359,191 + -0.6 1,359,191 + +0.1 1,359,404 + Pf6N2E2_1161 0.21 +2.0 1,359,404 + Pf6N2E2_1161 0.21 +0.5 1,359,414 + Pf6N2E2_1161 0.22 +0.4 1,359,414 + Pf6N2E2_1161 0.22 +0.8 1,359,415 - Pf6N2E2_1161 0.22 +0.1 1,359,415 - Pf6N2E2_1161 0.22 -0.6 1,359,650 + Pf6N2E2_1161 0.57 +0.4 1,359,651 - Pf6N2E2_1161 0.57 +0.1 1,359,651 - Pf6N2E2_1161 0.57 +1.1 1,359,824 - Pf6N2E2_1161 0.83 -0.1 1,359,824 - Pf6N2E2_1161 0.83 +0.7 1,359,974 + -0.2 1,360,071 - Pf6N2E2_1162 0.10 +0.9 1,360,071 - Pf6N2E2_1162 0.10 -0.5 1,360,071 - Pf6N2E2_1162 0.10 -0.1 1,360,515 + Pf6N2E2_1162 0.81 -2.4 1,360,613 + -0.3 1,360,613 + -0.7 1,360,614 - -0.6 1,360,891 - +0.1
Or see this region's nucleotide sequence