Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1102 and Pf6N2E2_1103 are separated by 11 nucleotides Pf6N2E2_1103 and Pf6N2E2_1104 overlap by 4 nucleotides Pf6N2E2_1104 and Pf6N2E2_1105 are separated by 17 nucleotides
Pf6N2E2_1102 - Aldehyde dehydrogenase A (EC 1.2.1.22), at 1,288,309 to 1,289,742
_1102
Pf6N2E2_1103 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.n4), at 1,289,754 to 1,290,554
_1103
Pf6N2E2_1104 - Gluconate dehydratase (EC 4.2.1.39), at 1,290,551 to 1,291,750
_1104
Pf6N2E2_1105 - Nitrate/nitrite transporter, at 1,291,768 to 1,293,063
_1105
Position (kb)
1290
1291
1292 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.669 kb on + strand at 1289.670 kb on - strand at 1289.758 kb on + strand at 1289.759 kb on - strand at 1289.973 kb on + strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1289.974 kb on - strand, within Pf6N2E2_1103 at 1290.145 kb on + strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.146 kb on - strand, within Pf6N2E2_1103 at 1290.359 kb on - strand, within Pf6N2E2_1103 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.755 kb on - strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.777 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.783 kb on + strand, within Pf6N2E2_1104 at 1290.784 kb on - strand, within Pf6N2E2_1104 at 1290.807 kb on - strand, within Pf6N2E2_1104 at 1290.977 kb on + strand, within Pf6N2E2_1104 at 1291.083 kb on + strand, within Pf6N2E2_1104 at 1291.084 kb on - strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.410 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.425 kb on + strand, within Pf6N2E2_1104 at 1291.426 kb on - strand, within Pf6N2E2_1104 at 1291.503 kb on + strand, within Pf6N2E2_1104 at 1291.629 kb on + strand, within Pf6N2E2_1104 at 1291.744 kb on + strand at 1291.744 kb on + strand at 1291.750 kb on + strand at 1291.750 kb on + strand at 1291.750 kb on + strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.751 kb on - strand at 1291.769 kb on + strand at 1291.770 kb on - strand at 1291.862 kb on + strand at 1291.863 kb on - strand at 1292.184 kb on - strand, within Pf6N2E2_1105 at 1292.283 kb on - strand, within Pf6N2E2_1105 at 1292.297 kb on + strand, within Pf6N2E2_1105 at 1292.322 kb on - strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.336 kb on + strand, within Pf6N2E2_1105 at 1292.337 kb on - strand, within Pf6N2E2_1105 at 1292.480 kb on + strand, within Pf6N2E2_1105 at 1292.486 kb on + strand, within Pf6N2E2_1105 at 1292.631 kb on - strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.675 kb on + strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.676 kb on - strand, within Pf6N2E2_1105 at 1292.684 kb on + strand, within Pf6N2E2_1105 at 1292.684 kb on + strand, within Pf6N2E2_1105 at 1292.685 kb on - strand, within Pf6N2E2_1105
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 1,289,669 + +0.6 1,289,669 + +0.6 1,289,669 + +0.3 1,289,669 + -0.2 1,289,669 + -0.1 1,289,670 - +0.5 1,289,758 + -0.1 1,289,759 - +0.7 1,289,973 + Pf6N2E2_1103 0.27 -0.0 1,289,974 - Pf6N2E2_1103 0.27 +0.9 1,289,974 - Pf6N2E2_1103 0.27 -0.5 1,290,145 + Pf6N2E2_1103 0.49 +0.3 1,290,146 - Pf6N2E2_1103 0.49 +0.4 1,290,146 - Pf6N2E2_1103 0.49 -0.9 1,290,146 - Pf6N2E2_1103 0.49 +0.2 1,290,359 - Pf6N2E2_1103 0.76 +0.5 1,290,755 - Pf6N2E2_1104 0.17 +0.8 1,290,755 - Pf6N2E2_1104 0.17 +1.9 1,290,755 - Pf6N2E2_1104 0.17 +0.7 1,290,777 + Pf6N2E2_1104 0.19 -1.9 1,290,777 + Pf6N2E2_1104 0.19 +0.5 1,290,783 + Pf6N2E2_1104 0.19 +0.4 1,290,783 + Pf6N2E2_1104 0.19 +1.1 1,290,784 - Pf6N2E2_1104 0.19 +0.7 1,290,807 - Pf6N2E2_1104 0.21 +0.6 1,290,977 + Pf6N2E2_1104 0.35 +0.8 1,291,083 + Pf6N2E2_1104 0.44 +0.3 1,291,084 - Pf6N2E2_1104 0.44 -0.0 1,291,410 + Pf6N2E2_1104 0.72 +0.2 1,291,410 + Pf6N2E2_1104 0.72 -0.1 1,291,410 + Pf6N2E2_1104 0.72 +1.7 1,291,410 + Pf6N2E2_1104 0.72 -0.4 1,291,425 + Pf6N2E2_1104 0.73 -0.1 1,291,425 + Pf6N2E2_1104 0.73 +0.1 1,291,425 + Pf6N2E2_1104 0.73 +2.7 1,291,425 + Pf6N2E2_1104 0.73 +1.3 1,291,426 - Pf6N2E2_1104 0.73 +1.2 1,291,503 + Pf6N2E2_1104 0.79 +0.2 1,291,629 + Pf6N2E2_1104 0.90 +0.5 1,291,744 + +0.4 1,291,744 + -0.5 1,291,750 + +0.1 1,291,750 + +0.8 1,291,750 + +0.7 1,291,751 - +0.4 1,291,751 - -1.2 1,291,751 - +0.5 1,291,751 - -0.3 1,291,751 - -0.5 1,291,751 - +0.2 1,291,751 - +0.1 1,291,769 + +0.3 1,291,770 - +0.7 1,291,862 + +1.2 1,291,863 - +0.5 1,292,184 - Pf6N2E2_1105 0.32 +0.5 1,292,283 - Pf6N2E2_1105 0.40 +0.9 1,292,297 + Pf6N2E2_1105 0.41 -0.1 1,292,322 - Pf6N2E2_1105 0.43 +0.2 1,292,336 + Pf6N2E2_1105 0.44 -0.8 1,292,336 + Pf6N2E2_1105 0.44 +0.1 1,292,336 + Pf6N2E2_1105 0.44 -0.3 1,292,336 + Pf6N2E2_1105 0.44 -0.2 1,292,337 - Pf6N2E2_1105 0.44 -0.2 1,292,480 + Pf6N2E2_1105 0.55 +0.7 1,292,486 + Pf6N2E2_1105 0.55 -0.9 1,292,631 - Pf6N2E2_1105 0.67 -1.7 1,292,675 + Pf6N2E2_1105 0.70 +1.4 1,292,675 + Pf6N2E2_1105 0.70 +0.3 1,292,675 + Pf6N2E2_1105 0.70 +0.5 1,292,675 + Pf6N2E2_1105 0.70 -0.3 1,292,675 + Pf6N2E2_1105 0.70 -0.1 1,292,675 + Pf6N2E2_1105 0.70 -0.4 1,292,676 - Pf6N2E2_1105 0.70 -1.3 1,292,676 - Pf6N2E2_1105 0.70 -1.8 1,292,676 - Pf6N2E2_1105 0.70 +1.0 1,292,684 + Pf6N2E2_1105 0.71 +1.2 1,292,684 + Pf6N2E2_1105 0.71 -0.5 1,292,685 - Pf6N2E2_1105 0.71 +0.7
Or see this region's nucleotide sequence