Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1021

Experiment: Sisomicin 0.0016 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1020 and Pf6N2E2_1021 are separated by 397 nucleotidesPf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides Pf6N2E2_1020 - TonB-dependent receptor, at 1,191,280 to 1,193,481 _1020 Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826 _1021 Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022 _1022 Position (kb) 1193 1194 1195Strain fitness (log2 ratio) -2 -1 0 1 2at 1192.880 kb on + strand, within Pf6N2E2_1020at 1193.057 kb on + strand, within Pf6N2E2_1020at 1193.057 kb on + strand, within Pf6N2E2_1020at 1193.057 kb on + strand, within Pf6N2E2_1020at 1193.058 kb on - strand, within Pf6N2E2_1020at 1193.058 kb on - strand, within Pf6N2E2_1020at 1193.058 kb on - strand, within Pf6N2E2_1020at 1193.113 kb on + strand, within Pf6N2E2_1020at 1193.130 kb on - strand, within Pf6N2E2_1020at 1193.144 kb on + strand, within Pf6N2E2_1020at 1193.145 kb on - strand, within Pf6N2E2_1020at 1193.392 kb on - strandat 1193.628 kb on + strandat 1193.734 kb on + strandat 1193.883 kb on + strandat 1193.895 kb on - strandat 1193.895 kb on - strandat 1193.998 kb on + strand, within Pf6N2E2_1021at 1193.999 kb on - strand, within Pf6N2E2_1021at 1193.999 kb on - strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.030 kb on + strand, within Pf6N2E2_1021at 1194.031 kb on - strand, within Pf6N2E2_1021at 1194.031 kb on - strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.409 kb on + strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.410 kb on - strand, within Pf6N2E2_1021at 1194.459 kb on - strand, within Pf6N2E2_1021at 1194.583 kb on - strand, within Pf6N2E2_1021at 1194.628 kb on - strand, within Pf6N2E2_1021at 1195.244 kb on + strand, within Pf6N2E2_1022at 1195.366 kb on + strand, within Pf6N2E2_1022at 1195.396 kb on + strand, within Pf6N2E2_1022at 1195.733 kb on + strand, within Pf6N2E2_1022

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml
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1,192,880 + Pf6N2E2_1020 0.73 +0.3
1,193,057 + Pf6N2E2_1020 0.81 +0.8
1,193,057 + Pf6N2E2_1020 0.81 -0.1
1,193,057 + Pf6N2E2_1020 0.81 +0.6
1,193,058 - Pf6N2E2_1020 0.81 -0.1
1,193,058 - Pf6N2E2_1020 0.81 +1.2
1,193,058 - Pf6N2E2_1020 0.81 -1.3
1,193,113 + Pf6N2E2_1020 0.83 +0.3
1,193,130 - Pf6N2E2_1020 0.84 -0.2
1,193,144 + Pf6N2E2_1020 0.85 -1.5
1,193,145 - Pf6N2E2_1020 0.85 +1.2
1,193,392 - +0.4
1,193,628 + -0.2
1,193,734 + +1.2
1,193,883 + +0.9
1,193,895 - -0.6
1,193,895 - -0.6
1,193,998 + Pf6N2E2_1021 0.13 -0.3
1,193,999 - Pf6N2E2_1021 0.13 +0.5
1,193,999 - Pf6N2E2_1021 0.13 +1.6
1,194,030 + Pf6N2E2_1021 0.16 +0.2
1,194,030 + Pf6N2E2_1021 0.16 -0.0
1,194,030 + Pf6N2E2_1021 0.16 +0.7
1,194,031 - Pf6N2E2_1021 0.16 +1.3
1,194,031 - Pf6N2E2_1021 0.16 +0.9
1,194,409 + Pf6N2E2_1021 0.56 -0.0
1,194,409 + Pf6N2E2_1021 0.56 +0.7
1,194,409 + Pf6N2E2_1021 0.56 -0.0
1,194,410 - Pf6N2E2_1021 0.56 +2.2
1,194,410 - Pf6N2E2_1021 0.56 +0.7
1,194,410 - Pf6N2E2_1021 0.56 -1.8
1,194,459 - Pf6N2E2_1021 0.61 +0.4
1,194,583 - Pf6N2E2_1021 0.74 -0.9
1,194,628 - Pf6N2E2_1021 0.79 +1.1
1,195,244 + Pf6N2E2_1022 0.34 -0.3
1,195,366 + Pf6N2E2_1022 0.45 -0.3
1,195,396 + Pf6N2E2_1022 0.47 -0.4
1,195,733 + Pf6N2E2_1022 0.76 +0.4

Or see this region's nucleotide sequence