Experiment: Sisomicin 0.0016 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1020 and Pf6N2E2_1021 are separated by 397 nucleotides Pf6N2E2_1021 and Pf6N2E2_1022 are separated by 8 nucleotides
Pf6N2E2_1020 - TonB-dependent receptor, at 1,191,280 to 1,193,481
_1020
Pf6N2E2_1021 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 1,193,879 to 1,194,826
_1021
Pf6N2E2_1022 - Butyryl-CoA dehydrogenase (EC 1.3.99.2), at 1,194,835 to 1,196,022
_1022
Position (kb)
1193
1194
1195 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1192.880 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.057 kb on + strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.058 kb on - strand, within Pf6N2E2_1020 at 1193.113 kb on + strand, within Pf6N2E2_1020 at 1193.130 kb on - strand, within Pf6N2E2_1020 at 1193.144 kb on + strand, within Pf6N2E2_1020 at 1193.145 kb on - strand, within Pf6N2E2_1020 at 1193.392 kb on - strand at 1193.628 kb on + strand at 1193.734 kb on + strand at 1193.883 kb on + strand at 1193.895 kb on - strand at 1193.895 kb on - strand at 1193.998 kb on + strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1193.999 kb on - strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.030 kb on + strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.031 kb on - strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.409 kb on + strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.410 kb on - strand, within Pf6N2E2_1021 at 1194.459 kb on - strand, within Pf6N2E2_1021 at 1194.583 kb on - strand, within Pf6N2E2_1021 at 1194.628 kb on - strand, within Pf6N2E2_1021 at 1195.244 kb on + strand, within Pf6N2E2_1022 at 1195.366 kb on + strand, within Pf6N2E2_1022 at 1195.396 kb on + strand, within Pf6N2E2_1022 at 1195.733 kb on + strand, within Pf6N2E2_1022
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0016 mg/ml remove 1,192,880 + Pf6N2E2_1020 0.73 +0.3 1,193,057 + Pf6N2E2_1020 0.81 +0.8 1,193,057 + Pf6N2E2_1020 0.81 -0.1 1,193,057 + Pf6N2E2_1020 0.81 +0.6 1,193,058 - Pf6N2E2_1020 0.81 -0.1 1,193,058 - Pf6N2E2_1020 0.81 +1.2 1,193,058 - Pf6N2E2_1020 0.81 -1.3 1,193,113 + Pf6N2E2_1020 0.83 +0.3 1,193,130 - Pf6N2E2_1020 0.84 -0.2 1,193,144 + Pf6N2E2_1020 0.85 -1.5 1,193,145 - Pf6N2E2_1020 0.85 +1.2 1,193,392 - +0.4 1,193,628 + -0.2 1,193,734 + +1.2 1,193,883 + +0.9 1,193,895 - -0.6 1,193,895 - -0.6 1,193,998 + Pf6N2E2_1021 0.13 -0.3 1,193,999 - Pf6N2E2_1021 0.13 +0.5 1,193,999 - Pf6N2E2_1021 0.13 +1.6 1,194,030 + Pf6N2E2_1021 0.16 +0.2 1,194,030 + Pf6N2E2_1021 0.16 -0.0 1,194,030 + Pf6N2E2_1021 0.16 +0.7 1,194,031 - Pf6N2E2_1021 0.16 +1.3 1,194,031 - Pf6N2E2_1021 0.16 +0.9 1,194,409 + Pf6N2E2_1021 0.56 -0.0 1,194,409 + Pf6N2E2_1021 0.56 +0.7 1,194,409 + Pf6N2E2_1021 0.56 -0.0 1,194,410 - Pf6N2E2_1021 0.56 +2.2 1,194,410 - Pf6N2E2_1021 0.56 +0.7 1,194,410 - Pf6N2E2_1021 0.56 -1.8 1,194,459 - Pf6N2E2_1021 0.61 +0.4 1,194,583 - Pf6N2E2_1021 0.74 -0.9 1,194,628 - Pf6N2E2_1021 0.79 +1.1 1,195,244 + Pf6N2E2_1022 0.34 -0.3 1,195,366 + Pf6N2E2_1022 0.45 -0.3 1,195,396 + Pf6N2E2_1022 0.47 -0.4 1,195,733 + Pf6N2E2_1022 0.76 +0.4
Or see this region's nucleotide sequence