Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_869 and Pf6N2E2_870 are separated by 182 nucleotides Pf6N2E2_870 and Pf6N2E2_871 overlap by 4 nucleotides
Pf6N2E2_869 - FMN oxidoreductase, at 1,017,501 to 1,018,619
_869
Pf6N2E2_870 - DNA-binding response regulator, at 1,018,802 to 1,019,533
_870
Pf6N2E2_871 - sensor histidine kinase, at 1,019,530 to 1,020,912
_871
Position (kb)
1018
1019
1020 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1017.840 kb on + strand, within Pf6N2E2_869 at 1018.026 kb on + strand, within Pf6N2E2_869 at 1018.026 kb on + strand, within Pf6N2E2_869 at 1018.056 kb on - strand, within Pf6N2E2_869 at 1018.201 kb on - strand, within Pf6N2E2_869 at 1018.513 kb on + strand at 1018.603 kb on + strand at 1018.666 kb on + strand at 1018.666 kb on + strand at 1018.666 kb on + strand at 1018.666 kb on + strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.667 kb on - strand at 1018.708 kb on - strand at 1018.732 kb on + strand at 1018.754 kb on + strand at 1018.755 kb on - strand at 1019.137 kb on - strand, within Pf6N2E2_870 at 1019.217 kb on - strand, within Pf6N2E2_870 at 1019.320 kb on - strand, within Pf6N2E2_870 at 1019.320 kb on - strand, within Pf6N2E2_870 at 1019.320 kb on - strand, within Pf6N2E2_870 at 1019.409 kb on + strand, within Pf6N2E2_870 at 1019.409 kb on + strand, within Pf6N2E2_870 at 1019.410 kb on - strand, within Pf6N2E2_870 at 1019.410 kb on - strand, within Pf6N2E2_870 at 1019.476 kb on + strand at 1019.610 kb on + strand at 1019.610 kb on + strand at 1019.611 kb on - strand at 1019.611 kb on - strand at 1020.031 kb on + strand, within Pf6N2E2_871 at 1020.032 kb on - strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand at 1020.309 kb on + strand, within Pf6N2E2_871 at 1020.479 kb on + strand, within Pf6N2E2_871
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 1,017,840 + Pf6N2E2_869 0.30 -0.1 1,018,026 + Pf6N2E2_869 0.47 -1.9 1,018,026 + Pf6N2E2_869 0.47 +0.7 1,018,056 - Pf6N2E2_869 0.50 -0.7 1,018,201 - Pf6N2E2_869 0.63 -0.9 1,018,513 + +1.3 1,018,603 + -0.3 1,018,666 + +0.5 1,018,666 + -1.3 1,018,666 + +1.4 1,018,666 + +0.6 1,018,667 - +0.3 1,018,667 - +0.1 1,018,667 - -0.6 1,018,667 - +1.0 1,018,667 - -0.7 1,018,667 - +0.4 1,018,667 - -0.7 1,018,667 - -0.2 1,018,708 - -0.0 1,018,732 + -0.3 1,018,754 + +2.5 1,018,755 - -0.2 1,019,137 - Pf6N2E2_870 0.46 -0.5 1,019,217 - Pf6N2E2_870 0.57 -0.3 1,019,320 - Pf6N2E2_870 0.71 -0.3 1,019,320 - Pf6N2E2_870 0.71 -2.1 1,019,320 - Pf6N2E2_870 0.71 -2.7 1,019,409 + Pf6N2E2_870 0.83 +0.3 1,019,409 + Pf6N2E2_870 0.83 +0.0 1,019,410 - Pf6N2E2_870 0.83 -1.1 1,019,410 - Pf6N2E2_870 0.83 -0.4 1,019,476 + +0.6 1,019,610 + +1.1 1,019,610 + -0.0 1,019,611 - -0.4 1,019,611 - +0.3 1,020,031 + Pf6N2E2_871 0.36 -1.0 1,020,032 - Pf6N2E2_871 0.36 +0.0 1,020,055 + Pf6N2E2_871 0.38 +1.3 1,020,055 + Pf6N2E2_871 0.38 -0.4 1,020,055 + Pf6N2E2_871 0.38 -1.5 1,020,055 + Pf6N2E2_871 0.38 +0.6 1,020,055 + Pf6N2E2_871 0.38 -0.2 1,020,055 + Pf6N2E2_871 0.38 -0.3 1,020,056 - Pf6N2E2_871 0.38 +0.9 1,020,056 - Pf6N2E2_871 0.38 +0.6 1,020,056 - Pf6N2E2_871 0.38 -0.3 1,020,056 - Pf6N2E2_871 0.38 -1.1 1,020,056 - -0.3 1,020,309 + Pf6N2E2_871 0.56 -0.3 1,020,479 + Pf6N2E2_871 0.69 +0.4
Or see this region's nucleotide sequence