Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_621 and Pf6N2E2_622 overlap by 4 nucleotides Pf6N2E2_622 and Pf6N2E2_623 overlap by 8 nucleotides Pf6N2E2_623 and Pf6N2E2_624 overlap by 4 nucleotides
Pf6N2E2_621 - TPR domain protein in aerotolerance operon, at 707,263 to 708,999
_621
Pf6N2E2_622 - BatA (Bacteroides aerotolerance operon), at 708,996 to 710,075
_622
Pf6N2E2_623 - FIG00954674: hypothetical protein, at 710,068 to 710,562
_623
Pf6N2E2_624 - hypothetical protein PA3071, at 710,559 to 711,503
_624
Position (kb) 708
709
710
711 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 707.996 kb on - strand, within Pf6N2E2_621 at 708.275 kb on + strand, within Pf6N2E2_621 at 708.567 kb on + strand, within Pf6N2E2_621 at 708.634 kb on + strand, within Pf6N2E2_621 at 708.634 kb on + strand, within Pf6N2E2_621 at 708.635 kb on - strand, within Pf6N2E2_621 at 708.861 kb on + strand at 708.862 kb on - strand at 708.862 kb on - strand at 708.950 kb on - strand at 708.950 kb on - strand at 708.958 kb on + strand at 708.959 kb on - strand at 709.165 kb on + strand, within Pf6N2E2_622 at 709.166 kb on - strand, within Pf6N2E2_622 at 709.245 kb on + strand, within Pf6N2E2_622 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.639 kb on + strand, within Pf6N2E2_622 at 709.640 kb on - strand, within Pf6N2E2_622 at 710.168 kb on - strand, within Pf6N2E2_623 at 710.628 kb on - strand at 711.067 kb on + strand, within Pf6N2E2_624
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 707,996 - Pf6N2E2_621 0.42 -0.7 708,275 + Pf6N2E2_621 0.58 -1.5 708,567 + Pf6N2E2_621 0.75 +0.0 708,634 + Pf6N2E2_621 0.79 -1.6 708,634 + Pf6N2E2_621 0.79 -0.9 708,635 - Pf6N2E2_621 0.79 +0.4 708,861 + -1.1 708,862 - +0.4 708,862 - -1.5 708,950 - -0.7 708,950 - -1.5 708,958 + -1.5 708,959 - -1.4 709,165 + Pf6N2E2_622 0.16 -0.1 709,166 - Pf6N2E2_622 0.16 -0.3 709,245 + Pf6N2E2_622 0.23 -2.9 709,639 + Pf6N2E2_622 0.60 -1.3 709,639 + Pf6N2E2_622 0.60 -0.6 709,640 - Pf6N2E2_622 0.60 +0.9 710,168 - Pf6N2E2_623 0.20 -0.2 710,628 - -1.3 711,067 + Pf6N2E2_624 0.54 -2.3
Or see this region's nucleotide sequence