Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2429 and Pf6N2E2_2430 are separated by 17 nucleotides Pf6N2E2_2430 and Pf6N2E2_2431 are separated by 3 nucleotides Pf6N2E2_2431 and Pf6N2E2_2432 are separated by 171 nucleotides Pf6N2E2_2432 and Pf6N2E2_2433 overlap by 1 nucleotides
Pf6N2E2_2429 - Glutathione-regulated potassium-efflux system protein KefB, at 2,877,443 to 2,879,155
_2429
Pf6N2E2_2430 - Ammonium transporter, at 2,879,173 to 2,880,381
_2430
Pf6N2E2_2431 - hypothetical protein, at 2,880,385 to 2,880,549
_2431
Pf6N2E2_2432 - Probable transmembrane protein, at 2,880,721 to 2,881,035
_2432
Pf6N2E2_2433 - Probable transmembrane protein, at 2,881,035 to 2,881,427
_2433
Position (kb)
2879
2880
2881 Strain fitness (log2 ratio)
-2
-1
0
1 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.224 kb on + strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.225 kb on - strand, within Pf6N2E2_2429 at 2878.371 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.536 kb on + strand, within Pf6N2E2_2429 at 2878.537 kb on - strand, within Pf6N2E2_2429 at 2878.753 kb on + strand, within Pf6N2E2_2429 at 2878.923 kb on + strand, within Pf6N2E2_2429 at 2879.126 kb on + strand at 2879.126 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.225 kb on + strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.226 kb on - strand at 2879.276 kb on + strand at 2879.276 kb on + strand at 2879.277 kb on - strand at 2879.620 kb on + strand, within Pf6N2E2_2430 at 2879.621 kb on - strand, within Pf6N2E2_2430 at 2879.979 kb on - strand, within Pf6N2E2_2430 at 2880.108 kb on + strand, within Pf6N2E2_2430 at 2880.108 kb on + strand, within Pf6N2E2_2430 at 2880.162 kb on - strand, within Pf6N2E2_2430 at 2880.162 kb on - strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.178 kb on + strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.179 kb on - strand, within Pf6N2E2_2430 at 2880.180 kb on + strand, within Pf6N2E2_2430 at 2880.189 kb on - strand, within Pf6N2E2_2430 at 2880.306 kb on + strand at 2880.306 kb on + strand at 2880.306 kb on + strand at 2880.307 kb on - strand at 2880.307 kb on - strand at 2880.384 kb on + strand at 2880.472 kb on - strand, within Pf6N2E2_2431 at 2880.552 kb on - strand at 2880.560 kb on + strand at 2880.617 kb on + strand at 2880.617 kb on + strand at 2880.617 kb on + strand at 2880.617 kb on + strand at 2880.617 kb on + strand at 2880.617 kb on + strand at 2880.618 kb on - strand at 2880.618 kb on - strand at 2880.618 kb on - strand at 2880.658 kb on + strand at 2880.658 kb on + strand at 2880.658 kb on + strand at 2880.658 kb on + strand at 2880.659 kb on - strand at 2880.682 kb on + strand at 2880.706 kb on + strand at 2880.952 kb on + strand, within Pf6N2E2_2432 at 2880.952 kb on + strand, within Pf6N2E2_2432 at 2880.952 kb on + strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.953 kb on - strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.956 kb on + strand, within Pf6N2E2_2432 at 2880.957 kb on - strand, within Pf6N2E2_2432 at 2880.957 kb on - strand, within Pf6N2E2_2432 at 2880.957 kb on - strand, within Pf6N2E2_2432 at 2880.957 kb on - strand, within Pf6N2E2_2432 at 2881.272 kb on + strand, within Pf6N2E2_2433 at 2881.272 kb on + strand, within Pf6N2E2_2433 at 2881.272 kb on + strand, within Pf6N2E2_2433 at 2881.273 kb on - strand, within Pf6N2E2_2433 at 2881.273 kb on - strand, within Pf6N2E2_2433 at 2881.273 kb on - strand, within Pf6N2E2_2433 at 2881.273 kb on - strand, within Pf6N2E2_2433 at 2881.308 kb on + strand, within Pf6N2E2_2433 at 2881.308 kb on + strand, within Pf6N2E2_2433 at 2881.308 kb on + strand, within Pf6N2E2_2433 at 2881.357 kb on - strand, within Pf6N2E2_2433 at 2881.357 kb on - strand, within Pf6N2E2_2433
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 2,878,224 + Pf6N2E2_2429 0.46 +0.6 2,878,224 + Pf6N2E2_2429 0.46 +0.5 2,878,224 + Pf6N2E2_2429 0.46 -1.4 2,878,224 + Pf6N2E2_2429 0.46 +0.4 2,878,225 - Pf6N2E2_2429 0.46 -0.2 2,878,225 - Pf6N2E2_2429 0.46 +1.1 2,878,225 - Pf6N2E2_2429 0.46 -0.3 2,878,225 - Pf6N2E2_2429 0.46 +0.8 2,878,225 - Pf6N2E2_2429 0.46 +0.3 2,878,225 - Pf6N2E2_2429 0.46 -0.8 2,878,371 + Pf6N2E2_2429 0.54 -0.1 2,878,536 + Pf6N2E2_2429 0.64 +0.5 2,878,536 + Pf6N2E2_2429 0.64 +1.2 2,878,536 + Pf6N2E2_2429 0.64 +0.5 2,878,537 - Pf6N2E2_2429 0.64 +0.1 2,878,753 + Pf6N2E2_2429 0.76 +0.8 2,878,923 + Pf6N2E2_2429 0.86 +0.3 2,879,126 + -0.1 2,879,126 + -0.8 2,879,225 + -0.4 2,879,225 + +0.7 2,879,225 + +0.9 2,879,225 + -0.3 2,879,225 + +0.5 2,879,225 + +1.1 2,879,226 - +0.4 2,879,226 - +0.2 2,879,226 - +0.1 2,879,226 - +0.3 2,879,226 - +0.3 2,879,226 - +0.5 2,879,276 + -0.5 2,879,276 + +1.1 2,879,277 - +0.6 2,879,620 + Pf6N2E2_2430 0.37 +0.1 2,879,621 - Pf6N2E2_2430 0.37 +0.8 2,879,979 - Pf6N2E2_2430 0.67 +0.2 2,880,108 + Pf6N2E2_2430 0.77 -0.9 2,880,108 + Pf6N2E2_2430 0.77 -0.7 2,880,162 - Pf6N2E2_2430 0.82 -0.6 2,880,162 - Pf6N2E2_2430 0.82 +0.6 2,880,178 + Pf6N2E2_2430 0.83 -1.5 2,880,178 + Pf6N2E2_2430 0.83 -0.1 2,880,178 + Pf6N2E2_2430 0.83 -0.3 2,880,178 + Pf6N2E2_2430 0.83 -0.1 2,880,178 + Pf6N2E2_2430 0.83 -0.2 2,880,178 + Pf6N2E2_2430 0.83 -0.5 2,880,179 - Pf6N2E2_2430 0.83 -0.1 2,880,179 - Pf6N2E2_2430 0.83 +0.3 2,880,179 - Pf6N2E2_2430 0.83 -0.7 2,880,179 - Pf6N2E2_2430 0.83 +0.1 2,880,179 - Pf6N2E2_2430 0.83 -0.0 2,880,179 - Pf6N2E2_2430 0.83 +0.3 2,880,179 - Pf6N2E2_2430 0.83 +0.2 2,880,179 - Pf6N2E2_2430 0.83 +0.1 2,880,180 + Pf6N2E2_2430 0.83 -0.7 2,880,189 - Pf6N2E2_2430 0.84 +0.3 2,880,306 + -0.4 2,880,306 + +0.2 2,880,306 + +0.6 2,880,307 - -0.9 2,880,307 - +0.1 2,880,384 + +0.2 2,880,472 - Pf6N2E2_2431 0.53 -0.0 2,880,552 - +0.1 2,880,560 + +1.1 2,880,617 + +0.1 2,880,617 + +0.4 2,880,617 + +0.5 2,880,617 + -0.9 2,880,617 + +0.4 2,880,617 + -0.4 2,880,618 - -0.1 2,880,618 - -0.1 2,880,618 - -0.2 2,880,658 + +1.7 2,880,658 + +0.1 2,880,658 + +0.4 2,880,658 + +0.8 2,880,659 - -0.6 2,880,682 + +0.4 2,880,706 + +0.7 2,880,952 + Pf6N2E2_2432 0.73 -0.4 2,880,952 + Pf6N2E2_2432 0.73 -0.1 2,880,952 + Pf6N2E2_2432 0.73 +0.7 2,880,953 - Pf6N2E2_2432 0.74 -0.1 2,880,953 - Pf6N2E2_2432 0.74 +0.1 2,880,953 - Pf6N2E2_2432 0.74 +0.3 2,880,953 - Pf6N2E2_2432 0.74 +0.4 2,880,953 - Pf6N2E2_2432 0.74 -1.1 2,880,953 - Pf6N2E2_2432 0.74 +0.7 2,880,953 - Pf6N2E2_2432 0.74 -0.2 2,880,953 - Pf6N2E2_2432 0.74 -1.0 2,880,953 - Pf6N2E2_2432 0.74 -1.3 2,880,953 - Pf6N2E2_2432 0.74 -1.1 2,880,956 + Pf6N2E2_2432 0.75 -0.9 2,880,956 + Pf6N2E2_2432 0.75 +0.8 2,880,956 + Pf6N2E2_2432 0.75 -0.5 2,880,956 + Pf6N2E2_2432 0.75 +0.1 2,880,956 + Pf6N2E2_2432 0.75 +0.2 2,880,956 + Pf6N2E2_2432 0.75 +0.7 2,880,956 + Pf6N2E2_2432 0.75 +1.3 2,880,957 - Pf6N2E2_2432 0.75 +0.2 2,880,957 - Pf6N2E2_2432 0.75 +0.5 2,880,957 - Pf6N2E2_2432 0.75 -0.3 2,880,957 - Pf6N2E2_2432 0.75 -1.3 2,881,272 + Pf6N2E2_2433 0.60 +0.1 2,881,272 + Pf6N2E2_2433 0.60 -1.3 2,881,272 + Pf6N2E2_2433 0.60 +0.3 2,881,273 - Pf6N2E2_2433 0.61 -1.9 2,881,273 - Pf6N2E2_2433 0.61 -0.8 2,881,273 - Pf6N2E2_2433 0.61 +1.1 2,881,273 - Pf6N2E2_2433 0.61 +0.3 2,881,308 + Pf6N2E2_2433 0.69 -0.5 2,881,308 + Pf6N2E2_2433 0.69 +0.3 2,881,308 + Pf6N2E2_2433 0.69 +0.1 2,881,357 - Pf6N2E2_2433 0.82 +0.3 2,881,357 - Pf6N2E2_2433 0.82 -0.7
Or see this region's nucleotide sequence