Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2349 and Pf6N2E2_2350 are separated by 4 nucleotides Pf6N2E2_2350 and Pf6N2E2_2351 overlap by 29 nucleotides Pf6N2E2_2351 and Pf6N2E2_2352 are separated by 4 nucleotides Pf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotides
Pf6N2E2_2349 - hypothetical protein, at 2,785,576 to 2,785,989
_2349
Pf6N2E2_2350 - Oxygen-regulated invasion protein OrgB, at 2,785,994 to 2,786,704
_2350
Pf6N2E2_2351 - Oxygen-regulated invasion protein OrgA, at 2,786,676 to 2,787,224
_2351
Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948
_2352
Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253
_2353
Position (kb)
2786
2787
2788 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2785.693 kb on - strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.694 kb on + strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.695 kb on - strand, within Pf6N2E2_2349 at 2785.738 kb on + strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.739 kb on - strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2785.871 kb on + strand, within Pf6N2E2_2349 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.205 kb on + strand, within Pf6N2E2_2350 at 2786.206 kb on - strand, within Pf6N2E2_2350 at 2786.223 kb on + strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.224 kb on - strand, within Pf6N2E2_2350 at 2786.920 kb on - strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.023 kb on + strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.024 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.026 kb on - strand, within Pf6N2E2_2351 at 2787.132 kb on + strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.133 kb on - strand, within Pf6N2E2_2351 at 2787.193 kb on + strand at 2787.194 kb on - strand at 2787.269 kb on + strand at 2787.413 kb on + strand, within Pf6N2E2_2352 at 2787.557 kb on + strand, within Pf6N2E2_2352 at 2787.558 kb on - strand, within Pf6N2E2_2352 at 2787.635 kb on + strand, within Pf6N2E2_2352 at 2787.636 kb on - strand, within Pf6N2E2_2352 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.024 kb on + strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.025 kb on - strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.056 kb on + strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353 at 2788.057 kb on - strand, within Pf6N2E2_2353
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 2,785,693 - Pf6N2E2_2349 0.28 -0.0 2,785,694 + Pf6N2E2_2349 0.29 +0.3 2,785,694 + Pf6N2E2_2349 0.29 -0.8 2,785,694 + Pf6N2E2_2349 0.29 -0.4 2,785,694 + Pf6N2E2_2349 0.29 -0.3 2,785,694 + Pf6N2E2_2349 0.29 +0.4 2,785,694 + Pf6N2E2_2349 0.29 -1.8 2,785,695 - Pf6N2E2_2349 0.29 -0.9 2,785,695 - Pf6N2E2_2349 0.29 +0.1 2,785,695 - Pf6N2E2_2349 0.29 +0.1 2,785,695 - Pf6N2E2_2349 0.29 +0.6 2,785,695 - Pf6N2E2_2349 0.29 -0.5 2,785,695 - Pf6N2E2_2349 0.29 -0.7 2,785,695 - Pf6N2E2_2349 0.29 -1.3 2,785,695 - Pf6N2E2_2349 0.29 -0.1 2,785,695 - Pf6N2E2_2349 0.29 -0.8 2,785,738 + Pf6N2E2_2349 0.39 -0.1 2,785,739 - Pf6N2E2_2349 0.39 -0.0 2,785,739 - Pf6N2E2_2349 0.39 +0.4 2,785,871 + Pf6N2E2_2349 0.71 +1.0 2,785,871 + Pf6N2E2_2349 0.71 -0.8 2,785,871 + Pf6N2E2_2349 0.71 +2.0 2,785,871 + Pf6N2E2_2349 0.71 -0.6 2,786,205 + Pf6N2E2_2350 0.30 +0.9 2,786,205 + Pf6N2E2_2350 0.30 -1.0 2,786,206 - Pf6N2E2_2350 0.30 -0.2 2,786,223 + Pf6N2E2_2350 0.32 +0.8 2,786,224 - Pf6N2E2_2350 0.32 -0.3 2,786,224 - Pf6N2E2_2350 0.32 -1.5 2,786,920 - Pf6N2E2_2351 0.44 -0.7 2,787,023 + Pf6N2E2_2351 0.63 -0.9 2,787,023 + Pf6N2E2_2351 0.63 -0.4 2,787,024 - Pf6N2E2_2351 0.63 -0.9 2,787,024 - Pf6N2E2_2351 0.63 +0.2 2,787,026 - Pf6N2E2_2351 0.64 -0.5 2,787,026 - Pf6N2E2_2351 0.64 -0.5 2,787,026 - Pf6N2E2_2351 0.64 +0.4 2,787,132 + Pf6N2E2_2351 0.83 -1.6 2,787,133 - Pf6N2E2_2351 0.83 -1.1 2,787,133 - Pf6N2E2_2351 0.83 -1.3 2,787,193 + +0.4 2,787,194 - -0.0 2,787,269 + +0.2 2,787,413 + Pf6N2E2_2352 0.26 -0.5 2,787,557 + Pf6N2E2_2352 0.46 -0.9 2,787,558 - Pf6N2E2_2352 0.46 +1.4 2,787,635 + Pf6N2E2_2352 0.56 -0.7 2,787,636 - Pf6N2E2_2352 0.57 +0.4 2,788,024 + Pf6N2E2_2353 0.26 +0.3 2,788,024 + Pf6N2E2_2353 0.26 +0.5 2,788,024 + Pf6N2E2_2353 0.26 +0.6 2,788,025 - Pf6N2E2_2353 0.26 -0.9 2,788,025 - Pf6N2E2_2353 0.26 -0.4 2,788,056 + Pf6N2E2_2353 0.36 -0.7 2,788,056 + Pf6N2E2_2353 0.36 +0.3 2,788,056 + Pf6N2E2_2353 0.36 +1.5 2,788,056 + Pf6N2E2_2353 0.36 +1.0 2,788,057 - Pf6N2E2_2353 0.36 -0.6 2,788,057 - Pf6N2E2_2353 0.36 +0.8
Or see this region's nucleotide sequence