Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_2351

Experiment: Sisomicin 0.0008 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_2349 and Pf6N2E2_2350 are separated by 4 nucleotidesPf6N2E2_2350 and Pf6N2E2_2351 overlap by 29 nucleotidesPf6N2E2_2351 and Pf6N2E2_2352 are separated by 4 nucleotidesPf6N2E2_2352 and Pf6N2E2_2353 overlap by 4 nucleotides Pf6N2E2_2349 - hypothetical protein, at 2,785,576 to 2,785,989 _2349 Pf6N2E2_2350 - Oxygen-regulated invasion protein OrgB, at 2,785,994 to 2,786,704 _2350 Pf6N2E2_2351 - Oxygen-regulated invasion protein OrgA, at 2,786,676 to 2,787,224 _2351 Pf6N2E2_2352 - Type III secretion bridge between inner and outermembrane lipoprotein (YscJ,HrcJ,EscJ, PscJ), at 2,787,229 to 2,787,948 _2352 Pf6N2E2_2353 - cell invasion protein - cytoplasmic, at 2,787,945 to 2,788,253 _2353 Position (kb) 2786 2787 2788Strain fitness (log2 ratio) -2 -1 0 1 2at 2785.693 kb on - strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.694 kb on + strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.695 kb on - strand, within Pf6N2E2_2349at 2785.738 kb on + strand, within Pf6N2E2_2349at 2785.739 kb on - strand, within Pf6N2E2_2349at 2785.739 kb on - strand, within Pf6N2E2_2349at 2785.871 kb on + strand, within Pf6N2E2_2349at 2785.871 kb on + strand, within Pf6N2E2_2349at 2785.871 kb on + strand, within Pf6N2E2_2349at 2785.871 kb on + strand, within Pf6N2E2_2349at 2786.205 kb on + strand, within Pf6N2E2_2350at 2786.205 kb on + strand, within Pf6N2E2_2350at 2786.206 kb on - strand, within Pf6N2E2_2350at 2786.223 kb on + strand, within Pf6N2E2_2350at 2786.224 kb on - strand, within Pf6N2E2_2350at 2786.224 kb on - strand, within Pf6N2E2_2350at 2786.920 kb on - strand, within Pf6N2E2_2351at 2787.023 kb on + strand, within Pf6N2E2_2351at 2787.023 kb on + strand, within Pf6N2E2_2351at 2787.024 kb on - strand, within Pf6N2E2_2351at 2787.024 kb on - strand, within Pf6N2E2_2351at 2787.026 kb on - strand, within Pf6N2E2_2351at 2787.026 kb on - strand, within Pf6N2E2_2351at 2787.026 kb on - strand, within Pf6N2E2_2351at 2787.132 kb on + strand, within Pf6N2E2_2351at 2787.133 kb on - strand, within Pf6N2E2_2351at 2787.133 kb on - strand, within Pf6N2E2_2351at 2787.193 kb on + strandat 2787.194 kb on - strandat 2787.269 kb on + strandat 2787.413 kb on + strand, within Pf6N2E2_2352at 2787.557 kb on + strand, within Pf6N2E2_2352at 2787.558 kb on - strand, within Pf6N2E2_2352at 2787.635 kb on + strand, within Pf6N2E2_2352at 2787.636 kb on - strand, within Pf6N2E2_2352at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.024 kb on + strand, within Pf6N2E2_2353at 2788.025 kb on - strand, within Pf6N2E2_2353at 2788.025 kb on - strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.056 kb on + strand, within Pf6N2E2_2353at 2788.057 kb on - strand, within Pf6N2E2_2353at 2788.057 kb on - strand, within Pf6N2E2_2353

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Per-strain Table

Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml
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2,785,693 - Pf6N2E2_2349 0.28 -0.0
2,785,694 + Pf6N2E2_2349 0.29 +0.3
2,785,694 + Pf6N2E2_2349 0.29 -0.8
2,785,694 + Pf6N2E2_2349 0.29 -0.4
2,785,694 + Pf6N2E2_2349 0.29 -0.3
2,785,694 + Pf6N2E2_2349 0.29 +0.4
2,785,694 + Pf6N2E2_2349 0.29 -1.8
2,785,695 - Pf6N2E2_2349 0.29 -0.9
2,785,695 - Pf6N2E2_2349 0.29 +0.1
2,785,695 - Pf6N2E2_2349 0.29 +0.1
2,785,695 - Pf6N2E2_2349 0.29 +0.6
2,785,695 - Pf6N2E2_2349 0.29 -0.5
2,785,695 - Pf6N2E2_2349 0.29 -0.7
2,785,695 - Pf6N2E2_2349 0.29 -1.3
2,785,695 - Pf6N2E2_2349 0.29 -0.1
2,785,695 - Pf6N2E2_2349 0.29 -0.8
2,785,738 + Pf6N2E2_2349 0.39 -0.1
2,785,739 - Pf6N2E2_2349 0.39 -0.0
2,785,739 - Pf6N2E2_2349 0.39 +0.4
2,785,871 + Pf6N2E2_2349 0.71 +1.0
2,785,871 + Pf6N2E2_2349 0.71 -0.8
2,785,871 + Pf6N2E2_2349 0.71 +2.0
2,785,871 + Pf6N2E2_2349 0.71 -0.6
2,786,205 + Pf6N2E2_2350 0.30 +0.9
2,786,205 + Pf6N2E2_2350 0.30 -1.0
2,786,206 - Pf6N2E2_2350 0.30 -0.2
2,786,223 + Pf6N2E2_2350 0.32 +0.8
2,786,224 - Pf6N2E2_2350 0.32 -0.3
2,786,224 - Pf6N2E2_2350 0.32 -1.5
2,786,920 - Pf6N2E2_2351 0.44 -0.7
2,787,023 + Pf6N2E2_2351 0.63 -0.9
2,787,023 + Pf6N2E2_2351 0.63 -0.4
2,787,024 - Pf6N2E2_2351 0.63 -0.9
2,787,024 - Pf6N2E2_2351 0.63 +0.2
2,787,026 - Pf6N2E2_2351 0.64 -0.5
2,787,026 - Pf6N2E2_2351 0.64 -0.5
2,787,026 - Pf6N2E2_2351 0.64 +0.4
2,787,132 + Pf6N2E2_2351 0.83 -1.6
2,787,133 - Pf6N2E2_2351 0.83 -1.1
2,787,133 - Pf6N2E2_2351 0.83 -1.3
2,787,193 + +0.4
2,787,194 - -0.0
2,787,269 + +0.2
2,787,413 + Pf6N2E2_2352 0.26 -0.5
2,787,557 + Pf6N2E2_2352 0.46 -0.9
2,787,558 - Pf6N2E2_2352 0.46 +1.4
2,787,635 + Pf6N2E2_2352 0.56 -0.7
2,787,636 - Pf6N2E2_2352 0.57 +0.4
2,788,024 + Pf6N2E2_2353 0.26 +0.3
2,788,024 + Pf6N2E2_2353 0.26 +0.5
2,788,024 + Pf6N2E2_2353 0.26 +0.6
2,788,025 - Pf6N2E2_2353 0.26 -0.9
2,788,025 - Pf6N2E2_2353 0.26 -0.4
2,788,056 + Pf6N2E2_2353 0.36 -0.7
2,788,056 + Pf6N2E2_2353 0.36 +0.3
2,788,056 + Pf6N2E2_2353 0.36 +1.5
2,788,056 + Pf6N2E2_2353 0.36 +1.0
2,788,057 - Pf6N2E2_2353 0.36 -0.6
2,788,057 - Pf6N2E2_2353 0.36 +0.8

Or see this region's nucleotide sequence