Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2226 and Pf6N2E2_2227 are separated by 26 nucleotides Pf6N2E2_2227 and Pf6N2E2_2228 are separated by 139 nucleotides Pf6N2E2_2228 and Pf6N2E2_2229 overlap by 4 nucleotides
Pf6N2E2_2226 - Integral membrane protein TerC, at 2,639,182 to 2,639,931
_2226
Pf6N2E2_2227 - Membrane protein involved in colicin uptake, at 2,639,958 to 2,640,209
_2227
Pf6N2E2_2228 - Transcriptional regulator, LysR family, at 2,640,349 to 2,641,245
_2228
Pf6N2E2_2229 - Major facilitator family transporter, at 2,641,242 to 2,642,393
_2229
Position (kb)
2640
2641
2642 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2639.917 kb on + strand at 2639.917 kb on + strand at 2639.918 kb on - strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.924 kb on + strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.925 kb on - strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.959 kb on + strand at 2639.960 kb on - strand at 2639.960 kb on - strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.202 kb on + strand at 2640.203 kb on - strand at 2640.203 kb on - strand at 2640.203 kb on - strand at 2640.233 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.274 kb on + strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.275 kb on - strand at 2640.373 kb on + strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.374 kb on - strand at 2640.390 kb on + strand at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.478 kb on + strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.479 kb on - strand, within Pf6N2E2_2228 at 2640.480 kb on + strand, within Pf6N2E2_2228 at 2640.481 kb on - strand, within Pf6N2E2_2228 at 2640.890 kb on - strand, within Pf6N2E2_2228 at 2641.006 kb on - strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.118 kb on + strand, within Pf6N2E2_2228 at 2641.119 kb on - strand, within Pf6N2E2_2228 at 2641.160 kb on - strand at 2641.160 kb on - strand at 2641.169 kb on - strand at 2641.243 kb on + strand at 2641.243 kb on + strand at 2641.244 kb on - strand at 2641.347 kb on - strand at 2641.644 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.729 kb on + strand, within Pf6N2E2_2229 at 2641.730 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.736 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.865 kb on - strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.868 kb on + strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2641.869 kb on - strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229 at 2642.180 kb on + strand, within Pf6N2E2_2229
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 2,639,917 + -0.9 2,639,917 + +1.9 2,639,918 - -0.7 2,639,924 + +0.2 2,639,924 + -1.2 2,639,924 + -1.0 2,639,924 + -0.6 2,639,925 - +0.2 2,639,925 - -0.1 2,639,925 - -1.0 2,639,925 - +0.5 2,639,925 - +0.1 2,639,959 + -0.2 2,639,959 + +0.4 2,639,959 + -0.9 2,639,959 + +0.3 2,639,960 - -0.5 2,639,960 - -0.7 2,640,202 + +0.4 2,640,202 + +0.6 2,640,202 + +1.1 2,640,202 + -0.3 2,640,202 + -0.2 2,640,203 - -0.5 2,640,203 - -1.2 2,640,203 - +0.6 2,640,233 + +0.5 2,640,274 + +0.5 2,640,274 + +1.2 2,640,274 + +0.1 2,640,274 + -1.4 2,640,275 - -0.8 2,640,275 - -0.0 2,640,275 - -0.1 2,640,373 + +0.4 2,640,374 - -0.3 2,640,374 - +0.2 2,640,374 - -0.7 2,640,390 + +1.0 2,640,478 + Pf6N2E2_2228 0.14 +0.4 2,640,478 + Pf6N2E2_2228 0.14 +0.6 2,640,478 + Pf6N2E2_2228 0.14 -1.2 2,640,478 + Pf6N2E2_2228 0.14 -0.8 2,640,479 - Pf6N2E2_2228 0.14 -1.2 2,640,479 - Pf6N2E2_2228 0.14 -0.9 2,640,479 - Pf6N2E2_2228 0.14 +0.1 2,640,479 - Pf6N2E2_2228 0.14 +0.9 2,640,479 - Pf6N2E2_2228 0.14 -1.0 2,640,480 + Pf6N2E2_2228 0.15 -0.7 2,640,481 - Pf6N2E2_2228 0.15 +0.3 2,640,890 - Pf6N2E2_2228 0.60 -0.3 2,641,006 - Pf6N2E2_2228 0.73 -0.8 2,641,118 + Pf6N2E2_2228 0.86 -0.5 2,641,118 + Pf6N2E2_2228 0.86 +0.1 2,641,118 + Pf6N2E2_2228 0.86 -1.3 2,641,119 - Pf6N2E2_2228 0.86 -0.6 2,641,160 - +0.9 2,641,160 - -0.3 2,641,169 - -0.1 2,641,243 + -0.1 2,641,243 + -2.0 2,641,244 - -0.0 2,641,347 - -0.0 2,641,644 + Pf6N2E2_2229 0.35 +0.4 2,641,729 + Pf6N2E2_2229 0.42 +0.2 2,641,729 + Pf6N2E2_2229 0.42 -0.3 2,641,730 - Pf6N2E2_2229 0.42 -1.5 2,641,736 - Pf6N2E2_2229 0.43 -0.3 2,641,736 - Pf6N2E2_2229 0.43 +0.5 2,641,736 - Pf6N2E2_2229 0.43 +0.2 2,641,865 - Pf6N2E2_2229 0.54 -0.2 2,641,865 - Pf6N2E2_2229 0.54 +1.7 2,641,865 - Pf6N2E2_2229 0.54 -1.1 2,641,868 + Pf6N2E2_2229 0.54 -1.1 2,641,868 + Pf6N2E2_2229 0.54 +0.1 2,641,869 - Pf6N2E2_2229 0.54 +0.2 2,641,869 - Pf6N2E2_2229 0.54 +0.8 2,642,180 + Pf6N2E2_2229 0.81 -1.4 2,642,180 + Pf6N2E2_2229 0.81 -1.1
Or see this region's nucleotide sequence