Experiment: Sisomicin 0.0008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1191 and Pf6N2E2_1192 are separated by 630 nucleotides Pf6N2E2_1192 and Pf6N2E2_1193 are separated by 135 nucleotides Pf6N2E2_1193 and Pf6N2E2_1194 are separated by 57 nucleotides
Pf6N2E2_1191 - Thiaminase II (EC 3.5.99.2), at 1,394,451 to 1,395,161
_1191
Pf6N2E2_1192 - Transcriptional regulator, at 1,395,792 to 1,396,019
_1192
Pf6N2E2_1193 - Outer membrane porin, OprD family, at 1,396,155 to 1,396,385
_1193
Pf6N2E2_1194 - hypothetical protein, at 1,396,443 to 1,397,384
_1194
Position (kb)
1396
1397 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.161 kb on + strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.162 kb on - strand at 1395.175 kb on + strand at 1395.176 kb on - strand at 1395.185 kb on + strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.186 kb on - strand at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.837 kb on + strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.838 kb on - strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.906 kb on + strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.907 kb on - strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1395.993 kb on + strand, within Pf6N2E2_1192 at 1396.000 kb on + strand at 1396.000 kb on + strand at 1396.104 kb on + strand at 1396.104 kb on + strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.105 kb on - strand at 1396.111 kb on - strand at 1396.158 kb on + strand at 1396.158 kb on + strand at 1396.220 kb on + strand, within Pf6N2E2_1193 at 1396.220 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.233 kb on + strand, within Pf6N2E2_1193 at 1396.234 kb on - strand, within Pf6N2E2_1193 at 1396.303 kb on + strand, within Pf6N2E2_1193 at 1396.328 kb on + strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.329 kb on - strand, within Pf6N2E2_1193 at 1396.352 kb on - strand, within Pf6N2E2_1193 at 1396.395 kb on - strand at 1396.395 kb on - strand at 1396.437 kb on - strand at 1396.509 kb on + strand at 1396.529 kb on + strand at 1396.614 kb on - strand, within Pf6N2E2_1194 at 1396.753 kb on - strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.798 kb on + strand, within Pf6N2E2_1194 at 1396.799 kb on - strand, within Pf6N2E2_1194 at 1396.807 kb on - strand, within Pf6N2E2_1194 at 1396.894 kb on + strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.895 kb on - strand, within Pf6N2E2_1194 at 1396.928 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.006 kb on - strand, within Pf6N2E2_1194 at 1397.029 kb on - strand, within Pf6N2E2_1194 at 1397.167 kb on - strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.223 kb on + strand, within Pf6N2E2_1194 at 1397.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Sisomicin 0.0008 mg/ml remove 1,395,161 + +0.1 1,395,161 + -1.2 1,395,161 + +0.4 1,395,162 - +0.9 1,395,162 - -0.2 1,395,162 - +0.4 1,395,175 + +0.3 1,395,176 - +2.0 1,395,185 + -0.7 1,395,186 - +0.4 1,395,186 - -0.1 1,395,186 - +0.7 1,395,837 + Pf6N2E2_1192 0.20 +0.1 1,395,837 + Pf6N2E2_1192 0.20 +0.8 1,395,838 - Pf6N2E2_1192 0.20 +0.3 1,395,838 - Pf6N2E2_1192 0.20 -0.4 1,395,906 + Pf6N2E2_1192 0.50 +0.4 1,395,906 + Pf6N2E2_1192 0.50 +0.2 1,395,906 + -0.9 1,395,906 + Pf6N2E2_1192 0.50 -0.1 1,395,906 + Pf6N2E2_1192 0.50 +1.8 1,395,907 - Pf6N2E2_1192 0.50 -0.4 1,395,907 - Pf6N2E2_1192 0.50 -0.4 1,395,907 - Pf6N2E2_1192 0.50 +1.6 1,395,907 - Pf6N2E2_1192 0.50 -0.8 1,395,993 + Pf6N2E2_1192 0.88 +0.1 1,395,993 + Pf6N2E2_1192 0.88 -0.1 1,396,000 + -0.7 1,396,000 + -0.5 1,396,104 + -0.1 1,396,104 + -0.3 1,396,105 - +2.1 1,396,105 - -0.8 1,396,105 - -1.5 1,396,111 - -1.1 1,396,158 + +1.2 1,396,158 + -0.7 1,396,220 + Pf6N2E2_1193 0.28 -0.7 1,396,220 + Pf6N2E2_1193 0.28 +0.1 1,396,233 + Pf6N2E2_1193 0.34 -0.9 1,396,233 + Pf6N2E2_1193 0.34 -1.6 1,396,233 + Pf6N2E2_1193 0.34 -0.4 1,396,234 - Pf6N2E2_1193 0.34 -0.4 1,396,303 + Pf6N2E2_1193 0.64 -0.5 1,396,328 + Pf6N2E2_1193 0.75 +0.4 1,396,329 - Pf6N2E2_1193 0.75 -1.4 1,396,329 - Pf6N2E2_1193 0.75 -2.5 1,396,329 - Pf6N2E2_1193 0.75 +0.1 1,396,352 - Pf6N2E2_1193 0.85 -0.2 1,396,395 - -0.5 1,396,395 - +0.4 1,396,437 - +0.4 1,396,509 + +1.2 1,396,529 + -0.9 1,396,614 - Pf6N2E2_1194 0.18 -0.3 1,396,753 - Pf6N2E2_1194 0.33 +0.2 1,396,798 + Pf6N2E2_1194 0.38 +0.4 1,396,798 + Pf6N2E2_1194 0.38 +0.9 1,396,799 - Pf6N2E2_1194 0.38 +0.1 1,396,807 - Pf6N2E2_1194 0.39 +0.5 1,396,894 + Pf6N2E2_1194 0.48 -0.0 1,396,895 - Pf6N2E2_1194 0.48 -0.4 1,396,895 - Pf6N2E2_1194 0.48 -0.8 1,396,928 - Pf6N2E2_1194 0.51 +0.3 1,397,006 - Pf6N2E2_1194 0.60 -1.7 1,397,006 - Pf6N2E2_1194 0.60 -1.3 1,397,006 - Pf6N2E2_1194 0.60 +0.4 1,397,029 - Pf6N2E2_1194 0.62 +1.1 1,397,167 - Pf6N2E2_1194 0.77 -0.6 1,397,223 + Pf6N2E2_1194 0.83 -0.2 1,397,223 + Pf6N2E2_1194 0.83 -1.5 1,397,223 + Pf6N2E2_1194 0.83 +0.5 1,397,383 - +0.8
Or see this region's nucleotide sequence