Experiment: A22 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17090 and BWI76_RS17095 are separated by 81 nucleotides BWI76_RS17095 and BWI76_RS17100 overlap by 1 nucleotides BWI76_RS17100 and BWI76_RS17105 are separated by 224 nucleotides
BWI76_RS17090: BWI76_RS17090 - NLP/P60 protein, at 3,546,764 to 3,547,228
_RS17090
BWI76_RS17095: BWI76_RS17095 - vitamin B12 ABC transporter ATP-binding protein BtuD, at 3,547,310 to 3,548,059
_RS17095
BWI76_RS17100: BWI76_RS17100 - glutathione peroxidase, at 3,548,059 to 3,548,610
_RS17100
BWI76_RS17105: BWI76_RS17105 - methionine ABC transporter substrate-binding protein MetQ, at 3,548,835 to 3,549,635
_RS17105
Position (kb)
3548
3549 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3547.496 kb on + strand, within BWI76_RS17095 at 3547.564 kb on + strand, within BWI76_RS17095 at 3547.695 kb on + strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.766 kb on - strand, within BWI76_RS17095 at 3547.850 kb on + strand, within BWI76_RS17095 at 3548.026 kb on + strand at 3548.387 kb on - strand, within BWI76_RS17100 at 3548.402 kb on - strand, within BWI76_RS17100 at 3548.654 kb on + strand at 3548.743 kb on - strand at 3548.856 kb on + strand at 3548.868 kb on + strand at 3548.882 kb on + strand at 3548.890 kb on - strand at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.086 kb on + strand, within BWI76_RS17105 at 3549.091 kb on + strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.094 kb on - strand, within BWI76_RS17105 at 3549.137 kb on + strand, within BWI76_RS17105 at 3549.375 kb on + strand, within BWI76_RS17105 at 3549.378 kb on - strand, within BWI76_RS17105 at 3549.382 kb on + strand, within BWI76_RS17105 at 3549.383 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.390 kb on - strand, within BWI76_RS17105 at 3549.441 kb on - strand at 3549.446 kb on - strand, within BWI76_RS17105 at 3549.492 kb on - strand, within BWI76_RS17105
Per-strain Table
Position Strand Gene LocusTag Fraction A22 0.1 mg/ml remove 3,547,496 + BWI76_RS17095 0.25 +0.7 3,547,564 + BWI76_RS17095 0.34 -0.2 3,547,695 + BWI76_RS17095 0.51 -0.2 3,547,766 - BWI76_RS17095 0.61 +0.6 3,547,766 - BWI76_RS17095 0.61 +0.2 3,547,850 + BWI76_RS17095 0.72 -0.0 3,548,026 + -0.3 3,548,387 - BWI76_RS17100 0.59 -0.7 3,548,402 - BWI76_RS17100 0.62 -0.6 3,548,654 + -0.1 3,548,743 - -0.1 3,548,856 + +0.5 3,548,868 + -0.1 3,548,882 + +0.8 3,548,890 - -0.3 3,549,086 + BWI76_RS17105 0.31 -0.7 3,549,086 + BWI76_RS17105 0.31 -0.3 3,549,091 + BWI76_RS17105 0.32 -0.6 3,549,094 - BWI76_RS17105 0.32 +0.0 3,549,094 - BWI76_RS17105 0.32 -0.5 3,549,137 + BWI76_RS17105 0.38 +0.6 3,549,375 + BWI76_RS17105 0.67 -0.1 3,549,378 - BWI76_RS17105 0.68 -0.0 3,549,382 + BWI76_RS17105 0.68 -0.7 3,549,383 - BWI76_RS17105 0.68 -0.4 3,549,390 - BWI76_RS17105 0.69 +0.7 3,549,390 - BWI76_RS17105 0.69 +0.1 3,549,441 - +3.1 3,549,446 - BWI76_RS17105 0.76 -0.7 3,549,492 - BWI76_RS17105 0.82 -0.5
Or see this region's nucleotide sequence