Strain Fitness in Caulobacter crescentus NA1000 around CCNA_R0076

Experiment: PYE 0.5x 25C survival rep1 28 days

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntCCNA_03310 and CCNA_03311 are separated by 10 nucleotidesCCNA_03311 and CCNA_R0076 are separated by 98 nucleotidesCCNA_R0076 and CCNA_03312 are separated by 234 nucleotidesCCNA_03312 and CCNA_03313 are separated by 16 nucleotides CCNA_03310: CCNA_03310 - transcription antitermination protein NusG, at 3,486,449 to 3,487,006 _03310 CCNA_03311: CCNA_03311 - protein translocase subunit SecE, at 3,487,017 to 3,487,334 _03311 CCNA_R0076: CCNA_R0076 - tRNA-Trp, at 3,487,433 to 3,487,508 _R0076 CCNA_03312: CCNA_03312 - hypothetical protein, at 3,487,743 to 3,487,922 _03312 CCNA_03313: CCNA_03313 - hypothetical protein, at 3,487,939 to 3,488,355 _03313 Position (kb) 3487 3488Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 3486.436 kb on + strandat 3486.436 kb on + strandat 3487.668 kb on + strandat 3487.668 kb on + strandat 3487.669 kb on + strandat 3487.669 kb on + strandat 3487.676 kb on - strandat 3487.676 kb on - strandat 3487.683 kb on + strandat 3487.691 kb on - strandat 3487.693 kb on + strandat 3487.696 kb on - strandat 3487.705 kb on + strandat 3487.825 kb on + strand, within CCNA_03312at 3487.835 kb on + strand, within CCNA_03312at 3487.843 kb on - strand, within CCNA_03312at 3487.884 kb on + strand, within CCNA_03312at 3487.890 kb on - strand, within CCNA_03312at 3487.899 kb on + strand, within CCNA_03312at 3487.899 kb on + strand, within CCNA_03312at 3487.907 kb on - strandat 3487.907 kb on - strandat 3487.926 kb on - strandat 3487.955 kb on + strandat 3487.963 kb on - strandat 3487.963 kb on - strandat 3488.033 kb on - strand, within CCNA_03313at 3488.072 kb on - strand, within CCNA_03313at 3488.075 kb on + strand, within CCNA_03313at 3488.082 kb on - strand, within CCNA_03313at 3488.083 kb on - strand, within CCNA_03313at 3488.089 kb on - strand, within CCNA_03313at 3488.089 kb on - strand, within CCNA_03313at 3488.126 kb on - strand, within CCNA_03313at 3488.142 kb on - strand, within CCNA_03313at 3488.151 kb on - strand, within CCNA_03313at 3488.169 kb on + strand, within CCNA_03313at 3488.185 kb on + strand, within CCNA_03313at 3488.212 kb on + strand, within CCNA_03313at 3488.212 kb on + strand, within CCNA_03313at 3488.220 kb on - strand, within CCNA_03313at 3488.240 kb on + strand, within CCNA_03313at 3488.261 kb on - strand, within CCNA_03313at 3488.264 kb on + strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.272 kb on - strand, within CCNA_03313at 3488.274 kb on - strand, within CCNA_03313at 3488.286 kb on + strand, within CCNA_03313at 3488.310 kb on + strand, within CCNA_03313at 3488.318 kb on - strandat 3488.318 kb on - strandat 3488.361 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction PYE 0.5x 25C survival rep1 28 days
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3,486,436 + -1.2
3,486,436 + -0.2
3,487,668 + -0.1
3,487,668 + -0.5
3,487,669 + -0.9
3,487,669 + -0.4
3,487,676 - -3.0
3,487,676 - -0.9
3,487,683 + +1.6
3,487,691 - +0.6
3,487,693 + -2.3
3,487,696 - -0.8
3,487,705 + -2.5
3,487,825 + CCNA_03312 0.46 +0.1
3,487,835 + CCNA_03312 0.51 -1.3
3,487,843 - CCNA_03312 0.56 -0.1
3,487,884 + CCNA_03312 0.78 +2.4
3,487,890 - CCNA_03312 0.82 +1.0
3,487,899 + CCNA_03312 0.87 -1.7
3,487,899 + CCNA_03312 0.87 -1.9
3,487,907 - +2.1
3,487,907 - -1.2
3,487,926 - -0.6
3,487,955 + -2.1
3,487,963 - -5.0
3,487,963 - -3.4
3,488,033 - CCNA_03313 0.23 -2.4
3,488,072 - CCNA_03313 0.32 -3.1
3,488,075 + CCNA_03313 0.33 -2.0
3,488,082 - CCNA_03313 0.34 +1.4
3,488,083 - CCNA_03313 0.35 -1.8
3,488,089 - CCNA_03313 0.36 -2.0
3,488,089 - CCNA_03313 0.36 -2.5
3,488,126 - CCNA_03313 0.45 -3.1
3,488,142 - CCNA_03313 0.49 -3.2
3,488,151 - CCNA_03313 0.51 -4.4
3,488,169 + CCNA_03313 0.55 -1.3
3,488,185 + CCNA_03313 0.59 -2.0
3,488,212 + CCNA_03313 0.65 -1.1
3,488,212 + CCNA_03313 0.65 -0.5
3,488,220 - CCNA_03313 0.67 -0.5
3,488,240 + CCNA_03313 0.72 +2.4
3,488,261 - CCNA_03313 0.77 -1.6
3,488,264 + CCNA_03313 0.78 +1.4
3,488,272 - CCNA_03313 0.80 -3.2
3,488,272 - CCNA_03313 0.80 -2.5
3,488,272 - CCNA_03313 0.80 -1.8
3,488,272 - CCNA_03313 0.80 -0.2
3,488,272 - CCNA_03313 0.80 -2.9
3,488,272 - CCNA_03313 0.80 -2.3
3,488,272 - CCNA_03313 0.80 +0.0
3,488,274 - CCNA_03313 0.80 -1.3
3,488,286 + CCNA_03313 0.83 -1.7
3,488,310 + CCNA_03313 0.89 -2.3
3,488,318 - -0.2
3,488,318 - -0.8
3,488,361 + +0.4

Or see this region's nucleotide sequence