Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22945 and BWI76_RS22950 overlap by 8 nucleotides BWI76_RS22950 and BWI76_RS22955 are separated by 19 nucleotides BWI76_RS22955 and BWI76_RS22960 are separated by 353 nucleotides BWI76_RS22960 and BWI76_RS22965 are separated by 198 nucleotides
BWI76_RS22945: BWI76_RS22945 - 23S rRNA (cytidine(2498)-2'-O)-methyltransferase RlmM, at 4,791,213 to 4,792,313
_RS22945
BWI76_RS22950: BWI76_RS22950 - membrane protein, at 4,792,306 to 4,792,701
_RS22950
BWI76_RS22955: BWI76_RS22955 - transcriptional regulator GcvA, at 4,792,721 to 4,793,638
_RS22955
BWI76_RS22960: BWI76_RS22960 - YgdI/YgdR family lipoprotein, at 4,793,992 to 4,794,216
_RS22960
BWI76_RS22965: BWI76_RS22965 - cysteine sulfinate desulfinase, at 4,794,415 to 4,795,620
_RS22965
Position (kb)
4792
4793
4794 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4792.024 kb on + strand, within BWI76_RS22945 at 4792.229 kb on + strand at 4792.393 kb on - strand, within BWI76_RS22950 at 4792.436 kb on - strand, within BWI76_RS22950 at 4792.704 kb on - strand at 4792.705 kb on - strand at 4792.705 kb on - strand at 4792.805 kb on - strand at 4793.060 kb on + strand, within BWI76_RS22955 at 4793.359 kb on + strand, within BWI76_RS22955 at 4793.402 kb on - strand, within BWI76_RS22955 at 4793.485 kb on + strand, within BWI76_RS22955 at 4793.485 kb on + strand, within BWI76_RS22955 at 4793.636 kb on - strand at 4793.832 kb on + strand at 4793.923 kb on - strand at 4794.029 kb on + strand, within BWI76_RS22960 at 4794.168 kb on + strand, within BWI76_RS22960 at 4794.176 kb on - strand, within BWI76_RS22960 at 4794.266 kb on + strand at 4794.269 kb on - strand at 4794.422 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 4,792,024 + BWI76_RS22945 0.74 -0.7 4,792,229 + -0.3 4,792,393 - BWI76_RS22950 0.22 -0.5 4,792,436 - BWI76_RS22950 0.33 -1.5 4,792,704 - -0.8 4,792,705 - -0.5 4,792,705 - -0.8 4,792,805 - +0.4 4,793,060 + BWI76_RS22955 0.37 -3.0 4,793,359 + BWI76_RS22955 0.69 -1.1 4,793,402 - BWI76_RS22955 0.74 -1.3 4,793,485 + BWI76_RS22955 0.83 -2.3 4,793,485 + BWI76_RS22955 0.83 -2.9 4,793,636 - -1.5 4,793,832 + -0.9 4,793,923 - -0.8 4,794,029 + BWI76_RS22960 0.16 -1.9 4,794,168 + BWI76_RS22960 0.78 +0.4 4,794,176 - BWI76_RS22960 0.82 +0.7 4,794,266 + -0.2 4,794,269 - -0.8 4,794,422 + -0.5
Or see this region's nucleotide sequence