Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22670

Experiment: Cephalothin 0.16 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22665 and BWI76_RS22670 overlap by 4 nucleotidesBWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotidesBWI76_RS22675 and BWI76_RS22680 are separated by 19 nucleotides BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906 _RS22665 BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382 _RS22670 BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092 _RS22675 BWI76_RS22680: BWI76_RS22680 - cell division protein FtsB, at 4,726,112 to 4,726,429 _RS22680 Position (kb) 4724 4725 4726Strain fitness (log2 ratio) -2 -1 0 1at 4724.108 kb on - strand, within BWI76_RS22665at 4724.504 kb on + strand, within BWI76_RS22665at 4724.504 kb on + strand, within BWI76_RS22665at 4724.512 kb on - strand, within BWI76_RS22665at 4724.614 kb on - strand, within BWI76_RS22665at 4724.841 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml
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4,724,108 - BWI76_RS22665 0.24 -2.4
4,724,504 + BWI76_RS22665 0.62 +0.5
4,724,504 + BWI76_RS22665 0.62 +0.9
4,724,512 - BWI76_RS22665 0.62 +0.3
4,724,614 - BWI76_RS22665 0.72 +0.4
4,724,841 + -1.9

Or see this region's nucleotide sequence