Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22655 and BWI76_RS22660 overlap by 7 nucleotides BWI76_RS22660 and BWI76_RS22665 overlap by 20 nucleotides BWI76_RS22665 and BWI76_RS22670 overlap by 4 nucleotides BWI76_RS22670 and BWI76_RS22675 overlap by 1 nucleotides
BWI76_RS22655: BWI76_RS22655 - protein-L-isoaspartate O-methyltransferase, at 4,722,495 to 4,723,121
_RS22655
BWI76_RS22660: BWI76_RS22660 - 5'/3'-nucleotidase SurE, at 4,723,115 to 4,723,876
_RS22660
BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906
_RS22665
BWI76_RS22670: BWI76_RS22670 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase, at 4,724,903 to 4,725,382
_RS22670
BWI76_RS22675: BWI76_RS22675 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, at 4,725,382 to 4,726,092
_RS22675
Position (kb) 4723
4724
4725 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4722.901 kb on - strand, within BWI76_RS22655 at 4722.933 kb on + strand, within BWI76_RS22655 at 4722.941 kb on - strand at 4723.281 kb on + strand, within BWI76_RS22660 at 4723.289 kb on - strand, within BWI76_RS22660 at 4723.368 kb on + strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.463 kb on + strand, within BWI76_RS22660 at 4723.637 kb on + strand, within BWI76_RS22660 at 4723.657 kb on + strand, within BWI76_RS22660 at 4723.665 kb on - strand, within BWI76_RS22660 at 4723.685 kb on - strand, within BWI76_RS22660 at 4723.831 kb on - strand at 4724.108 kb on - strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.512 kb on - strand, within BWI76_RS22665 at 4724.614 kb on - strand, within BWI76_RS22665 at 4724.841 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 4,722,901 - BWI76_RS22655 0.65 -0.5 4,722,933 + BWI76_RS22655 0.70 +1.1 4,722,941 - +2.0 4,723,281 + BWI76_RS22660 0.22 -0.3 4,723,289 - BWI76_RS22660 0.23 +0.5 4,723,368 + BWI76_RS22660 0.33 +0.0 4,723,376 - BWI76_RS22660 0.34 -0.1 4,723,376 - BWI76_RS22660 0.34 -0.5 4,723,376 - BWI76_RS22660 0.34 -0.4 4,723,463 + BWI76_RS22660 0.46 +0.8 4,723,637 + BWI76_RS22660 0.69 +0.7 4,723,657 + BWI76_RS22660 0.71 +0.6 4,723,665 - BWI76_RS22660 0.72 +0.3 4,723,685 - BWI76_RS22660 0.75 -0.1 4,723,831 - +0.8 4,724,108 - BWI76_RS22665 0.24 -2.4 4,724,504 + BWI76_RS22665 0.62 +0.5 4,724,504 + BWI76_RS22665 0.62 +0.9 4,724,512 - BWI76_RS22665 0.62 +0.3 4,724,614 - BWI76_RS22665 0.72 +0.4 4,724,841 + -1.9
Or see this region's nucleotide sequence