Experiment: Cephalothin 0.16 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05355 and BWI76_RS05360 are separated by 39 nucleotides BWI76_RS05360 and BWI76_RS05365 overlap by 8 nucleotides BWI76_RS05365 and BWI76_RS05370 are separated by 188 nucleotides BWI76_RS05370 and BWI76_RS05375 are separated by 105 nucleotides
BWI76_RS05355: BWI76_RS05355 - methionine ABC transporter substrate-binding protein MetQ, at 1,127,149 to 1,127,964
_RS05355
BWI76_RS05360: BWI76_RS05360 - methionine ABC transporter permease, at 1,128,004 to 1,128,657
_RS05360
BWI76_RS05365: BWI76_RS05365 - D-methionine ABC transporter, ATP-binding protein, at 1,128,650 to 1,129,681
_RS05365
BWI76_RS05370: BWI76_RS05370 - D-glycero-beta-D-manno-heptose 1,7-bisphosphate 7-phosphatase, at 1,129,870 to 1,130,436
_RS05370
BWI76_RS05375: BWI76_RS05375 - hypothetical protein, at 1,130,542 to 1,130,747
_RS05375
Position (kb)
1128
1129
1130 Strain fitness (log2 ratio)
-1
0
1
2 at 1127.690 kb on + strand, within BWI76_RS05355 at 1127.693 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.698 kb on - strand, within BWI76_RS05355 at 1127.892 kb on + strand at 1127.945 kb on - strand at 1128.075 kb on + strand, within BWI76_RS05360 at 1128.081 kb on + strand, within BWI76_RS05360 at 1128.089 kb on + strand, within BWI76_RS05360 at 1128.111 kb on + strand, within BWI76_RS05360 at 1128.206 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.442 kb on - strand, within BWI76_RS05360 at 1128.691 kb on - strand at 1128.753 kb on - strand at 1128.753 kb on - strand at 1128.898 kb on - strand, within BWI76_RS05365 at 1129.073 kb on - strand, within BWI76_RS05365 at 1129.128 kb on + strand, within BWI76_RS05365 at 1129.283 kb on - strand, within BWI76_RS05365 at 1129.296 kb on - strand, within BWI76_RS05365 at 1129.413 kb on - strand, within BWI76_RS05365 at 1129.492 kb on + strand, within BWI76_RS05365 at 1129.492 kb on + strand, within BWI76_RS05365 at 1129.492 kb on + strand, within BWI76_RS05365 at 1129.497 kb on + strand, within BWI76_RS05365 at 1129.500 kb on - strand, within BWI76_RS05365 at 1129.502 kb on + strand, within BWI76_RS05365 at 1129.502 kb on + strand, within BWI76_RS05365 at 1129.510 kb on - strand, within BWI76_RS05365 at 1129.525 kb on + strand, within BWI76_RS05365 at 1129.525 kb on + strand, within BWI76_RS05365 at 1129.525 kb on + strand, within BWI76_RS05365 at 1129.533 kb on - strand, within BWI76_RS05365 at 1129.555 kb on - strand, within BWI76_RS05365
Per-strain Table
Position Strand Gene LocusTag Fraction Cephalothin 0.16 mg/ml remove 1,127,690 + BWI76_RS05355 0.66 -0.8 1,127,693 - BWI76_RS05355 0.67 +1.5 1,127,698 - BWI76_RS05355 0.67 +0.7 1,127,698 - BWI76_RS05355 0.67 +0.8 1,127,892 + +0.4 1,127,945 - +1.2 1,128,075 + BWI76_RS05360 0.11 +1.0 1,128,081 + BWI76_RS05360 0.12 +1.1 1,128,089 + BWI76_RS05360 0.13 +1.5 1,128,111 + BWI76_RS05360 0.16 -1.0 1,128,206 - BWI76_RS05360 0.31 -0.3 1,128,442 - BWI76_RS05360 0.67 -0.1 1,128,442 - BWI76_RS05360 0.67 -0.2 1,128,691 - -0.1 1,128,753 - -0.7 1,128,753 - -0.2 1,128,898 - BWI76_RS05365 0.24 +0.0 1,129,073 - BWI76_RS05365 0.41 +0.1 1,129,128 + BWI76_RS05365 0.46 +0.9 1,129,283 - BWI76_RS05365 0.61 +0.3 1,129,296 - BWI76_RS05365 0.63 +0.8 1,129,413 - BWI76_RS05365 0.74 -0.3 1,129,492 + BWI76_RS05365 0.82 +1.0 1,129,492 + BWI76_RS05365 0.82 +2.0 1,129,492 + BWI76_RS05365 0.82 +0.6 1,129,497 + BWI76_RS05365 0.82 -0.3 1,129,500 - BWI76_RS05365 0.82 +0.8 1,129,502 + BWI76_RS05365 0.83 +1.1 1,129,502 + BWI76_RS05365 0.83 +1.7 1,129,510 - BWI76_RS05365 0.83 +1.0 1,129,525 + BWI76_RS05365 0.85 +0.6 1,129,525 + BWI76_RS05365 0.85 -0.3 1,129,525 + BWI76_RS05365 0.85 +1.0 1,129,533 - BWI76_RS05365 0.86 +0.2 1,129,555 - BWI76_RS05365 0.88 +1.0
Or see this region's nucleotide sequence