Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_588 and Pf6N2E2_589 are separated by 89 nucleotides Pf6N2E2_589 and Pf6N2E2_590 are separated by 201 nucleotides Pf6N2E2_590 and Pf6N2E2_591 are separated by 327 nucleotides Pf6N2E2_591 and Pf6N2E2_592 overlap by 35 nucleotides
Pf6N2E2_588 - FIG005121: SAM-dependent methyltransferase (EC 2.1.1.-), at 665,855 to 666,613
_588
Pf6N2E2_589 - Ribonuclease HI (EC 3.1.26.4), at 666,703 to 667,155
_589
Pf6N2E2_590 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 667,357 to 668,118
_590
Pf6N2E2_591 - Streptothricin acetyltransferase, Streptomyces lavendulae type, at 668,446 to 669,060
_591
Pf6N2E2_592 - Transcriptional regulators, LysR family, at 669,026 to 669,919
_592
Position (kb) 667
668
669 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 666.489 kb on - strand, within Pf6N2E2_588 at 666.611 kb on + strand at 666.694 kb on + strand at 666.694 kb on + strand at 666.716 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand at 667.156 kb on + strand at 668.128 kb on - strand at 668.128 kb on - strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.154 kb on + strand at 668.155 kb on - strand at 668.155 kb on - strand at 668.155 kb on - strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.158 kb on + strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.159 kb on - strand at 668.201 kb on - strand at 668.285 kb on - strand at 668.329 kb on + strand at 668.329 kb on + strand at 668.330 kb on - strand at 668.330 kb on - strand at 668.330 kb on - strand at 668.445 kb on + strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.446 kb on - strand at 668.515 kb on + strand, within Pf6N2E2_591 at 668.515 kb on + strand, within Pf6N2E2_591 at 668.516 kb on - strand, within Pf6N2E2_591 at 668.517 kb on + strand, within Pf6N2E2_591 at 668.517 kb on + strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.518 kb on - strand, within Pf6N2E2_591 at 668.626 kb on + strand, within Pf6N2E2_591 at 668.626 kb on + strand, within Pf6N2E2_591 at 668.627 kb on - strand, within Pf6N2E2_591 at 668.692 kb on + strand, within Pf6N2E2_591 at 668.693 kb on - strand, within Pf6N2E2_591 at 668.693 kb on - strand, within Pf6N2E2_591 at 668.917 kb on + strand, within Pf6N2E2_591 at 668.918 kb on - strand, within Pf6N2E2_591 at 668.968 kb on - strand, within Pf6N2E2_591
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 666,489 - Pf6N2E2_588 0.84 -2.6 666,611 + +0.4 666,694 + +0.0 666,694 + -0.9 666,716 + -0.6 667,156 + -0.1 667,156 + -2.3 667,156 + +0.4 668,128 - +1.1 668,128 - -1.3 668,154 + -0.6 668,154 + -0.4 668,154 + -0.6 668,154 + +1.0 668,155 - +0.4 668,155 - +0.1 668,155 - +0.9 668,158 + -0.5 668,158 + -2.4 668,158 + +0.2 668,158 + +0.5 668,158 + +0.6 668,159 - +3.5 668,159 - +0.9 668,159 - -0.8 668,159 - -0.4 668,159 - +0.5 668,159 - +0.3 668,201 - +0.5 668,285 - -0.6 668,329 + +0.9 668,329 + -1.0 668,330 - -0.6 668,330 - +0.6 668,330 - +0.6 668,445 + +1.9 668,446 - -0.5 668,446 - -0.1 668,446 - -0.4 668,446 - -0.1 668,515 + Pf6N2E2_591 0.11 -0.9 668,515 + Pf6N2E2_591 0.11 +0.5 668,516 - Pf6N2E2_591 0.11 +0.8 668,517 + Pf6N2E2_591 0.12 -1.2 668,517 + Pf6N2E2_591 0.12 -1.2 668,518 - Pf6N2E2_591 0.12 -0.1 668,518 - Pf6N2E2_591 0.12 -1.6 668,518 - Pf6N2E2_591 0.12 -0.2 668,518 - Pf6N2E2_591 0.12 -0.8 668,518 - Pf6N2E2_591 0.12 +1.5 668,518 - Pf6N2E2_591 0.12 +0.4 668,626 + Pf6N2E2_591 0.29 +0.5 668,626 + Pf6N2E2_591 0.29 -0.6 668,627 - Pf6N2E2_591 0.29 -0.6 668,692 + Pf6N2E2_591 0.40 -1.6 668,693 - Pf6N2E2_591 0.40 +1.2 668,693 - Pf6N2E2_591 0.40 +0.6 668,917 + Pf6N2E2_591 0.77 -1.9 668,918 - Pf6N2E2_591 0.77 +0.2 668,968 - Pf6N2E2_591 0.85 +1.5
Or see this region's nucleotide sequence