Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5535 and Pf6N2E2_5536 are separated by 113 nucleotides Pf6N2E2_5536 and Pf6N2E2_5537 are separated by 0 nucleotides Pf6N2E2_5537 and Pf6N2E2_5538 are separated by 127 nucleotides Pf6N2E2_5538 and Pf6N2E2_5539 overlap by 11 nucleotides
Pf6N2E2_5535 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 6,351,872 to 6,352,396
_5535
Pf6N2E2_5536 - Holliday junction DNA helicase RuvA, at 6,352,510 to 6,353,118
_5536
Pf6N2E2_5537 - Holliday junction DNA helicase RuvB, at 6,353,119 to 6,354,180
_5537
Pf6N2E2_5538 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 6,354,308 to 6,354,775
_5538
Pf6N2E2_5539 - MotA/TolQ/ExbB proton channel family protein, at 6,354,765 to 6,355,460
_5539
Position (kb)
6353
6354
6355 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6352.572 kb on - strand, within Pf6N2E2_5536 at 6353.215 kb on + strand at 6354.052 kb on + strand, within Pf6N2E2_5537 at 6354.113 kb on + strand at 6354.114 kb on - strand at 6354.149 kb on + strand at 6354.295 kb on + strand at 6354.295 kb on + strand at 6354.302 kb on + strand at 6354.302 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.307 kb on + strand at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.356 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.359 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.389 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.392 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.401 kb on + strand, within Pf6N2E2_5538 at 6354.412 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.552 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.673 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.705 kb on + strand, within Pf6N2E2_5538 at 6354.739 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 6,352,572 - Pf6N2E2_5536 0.10 -0.1 6,353,215 + -2.5 6,354,052 + Pf6N2E2_5537 0.88 -2.2 6,354,113 + -1.0 6,354,114 - -3.2 6,354,149 + +1.1 6,354,295 + +0.6 6,354,295 + -0.0 6,354,302 + -1.4 6,354,302 + -1.0 6,354,307 + -1.1 6,354,307 + -1.0 6,354,307 + +1.0 6,354,356 + Pf6N2E2_5538 0.10 -0.5 6,354,356 + Pf6N2E2_5538 0.10 +1.1 6,354,359 + Pf6N2E2_5538 0.11 -1.0 6,354,359 + Pf6N2E2_5538 0.11 +0.3 6,354,389 + Pf6N2E2_5538 0.17 -0.9 6,354,389 + Pf6N2E2_5538 0.17 +0.0 6,354,389 + Pf6N2E2_5538 0.17 +0.9 6,354,392 + Pf6N2E2_5538 0.18 -1.0 6,354,392 + Pf6N2E2_5538 0.18 +0.4 6,354,392 + Pf6N2E2_5538 0.18 +0.2 6,354,392 + Pf6N2E2_5538 0.18 +0.6 6,354,401 + Pf6N2E2_5538 0.20 -1.0 6,354,401 + Pf6N2E2_5538 0.20 -0.1 6,354,412 + Pf6N2E2_5538 0.22 -0.7 6,354,552 + Pf6N2E2_5538 0.52 +0.1 6,354,552 + Pf6N2E2_5538 0.52 -0.4 6,354,673 + Pf6N2E2_5538 0.78 -0.0 6,354,673 + Pf6N2E2_5538 0.78 -0.3 6,354,705 + Pf6N2E2_5538 0.85 +0.4 6,354,705 + Pf6N2E2_5538 0.85 -3.1 6,354,739 + -2.5
Or see this region's nucleotide sequence