Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_4675

Experiment: Lomefloxacin 0.0002 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_4674 and Pf6N2E2_4675 are separated by 194 nucleotidesPf6N2E2_4675 and Pf6N2E2_4676 overlap by 1 nucleotides Pf6N2E2_4674 - Permeases of the major facilitator superfamily, at 5,405,600 to 5,407,027 _4674 Pf6N2E2_4675 - TldE/PmbA family protein, Beta/Gamma-proteobacterial subgroup, at 5,407,222 to 5,408,559 _4675 Pf6N2E2_4676 - TldD family protein, Beta/Gamma-proteobacterial subgroup, at 5,408,559 to 5,410,001 _4676 Position (kb) 5407 5408 5409Strain fitness (log2 ratio) -2 -1 0 1at 5406.291 kb on + strand, within Pf6N2E2_4674at 5406.291 kb on + strand, within Pf6N2E2_4674at 5406.480 kb on + strand, within Pf6N2E2_4674at 5406.481 kb on - strand, within Pf6N2E2_4674at 5406.538 kb on - strand, within Pf6N2E2_4674at 5406.855 kb on + strand, within Pf6N2E2_4674at 5406.855 kb on + strand, within Pf6N2E2_4674at 5406.855 kb on + strand, within Pf6N2E2_4674at 5406.856 kb on - strand, within Pf6N2E2_4674at 5406.856 kb on - strand, within Pf6N2E2_4674at 5407.039 kb on + strandat 5407.039 kb on + strandat 5407.039 kb on + strandat 5407.039 kb on + strandat 5407.039 kb on + strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.040 kb on - strandat 5407.064 kb on - strandat 5407.208 kb on + strandat 5407.223 kb on + strandat 5407.223 kb on + strandat 5407.223 kb on + strandat 5407.223 kb on + strandat 5407.224 kb on - strandat 5407.224 kb on - strandat 5407.224 kb on - strandat 5407.224 kb on - strandat 5407.224 kb on - strandat 5407.292 kb on + strandat 5407.292 kb on + strandat 5407.293 kb on - strandat 5407.293 kb on - strandat 5407.361 kb on + strand, within Pf6N2E2_4675at 5407.476 kb on - strand, within Pf6N2E2_4675at 5407.502 kb on + strand, within Pf6N2E2_4675at 5407.503 kb on - strand, within Pf6N2E2_4675at 5407.503 kb on - strand, within Pf6N2E2_4675at 5407.503 kb on - strand, within Pf6N2E2_4675at 5407.503 kb on - strand, within Pf6N2E2_4675at 5407.532 kb on + strand, within Pf6N2E2_4675at 5407.532 kb on + strand, within Pf6N2E2_4675at 5407.532 kb on + strand, within Pf6N2E2_4675at 5407.532 kb on + strand, within Pf6N2E2_4675at 5407.532 kb on + strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.533 kb on - strand, within Pf6N2E2_4675at 5407.583 kb on + strand, within Pf6N2E2_4675at 5407.583 kb on + strand, within Pf6N2E2_4675at 5407.583 kb on + strand, within Pf6N2E2_4675at 5407.584 kb on - strand, within Pf6N2E2_4675at 5407.584 kb on - strand, within Pf6N2E2_4675at 5407.584 kb on - strand, within Pf6N2E2_4675at 5407.880 kb on + strand, within Pf6N2E2_4675at 5407.880 kb on + strand, within Pf6N2E2_4675at 5407.881 kb on - strand, within Pf6N2E2_4675at 5407.998 kb on - strand, within Pf6N2E2_4675at 5407.998 kb on - strand, within Pf6N2E2_4675at 5408.123 kb on + strandat 5408.123 kb on + strand, within Pf6N2E2_4675at 5408.206 kb on + strand, within Pf6N2E2_4675at 5408.206 kb on + strand, within Pf6N2E2_4675at 5408.206 kb on + strand, within Pf6N2E2_4675at 5408.206 kb on + strand, within Pf6N2E2_4675at 5408.206 kb on + strand, within Pf6N2E2_4675at 5408.207 kb on - strand, within Pf6N2E2_4675at 5408.207 kb on - strand, within Pf6N2E2_4675at 5408.729 kb on + strand, within Pf6N2E2_4676at 5408.737 kb on + strand, within Pf6N2E2_4676at 5408.738 kb on - strand, within Pf6N2E2_4676at 5408.857 kb on - strand, within Pf6N2E2_4676at 5409.091 kb on + strand, within Pf6N2E2_4676at 5409.091 kb on + strand, within Pf6N2E2_4676at 5409.092 kb on - strand, within Pf6N2E2_4676at 5409.092 kb on - strand, within Pf6N2E2_4676at 5409.200 kb on - strand, within Pf6N2E2_4676at 5409.485 kb on - strand, within Pf6N2E2_4676at 5409.532 kb on + strand, within Pf6N2E2_4676at 5409.533 kb on - strand, within Pf6N2E2_4676

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Per-strain Table

Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM
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5,406,291 + Pf6N2E2_4674 0.48 -0.1
5,406,291 + Pf6N2E2_4674 0.48 -0.2
5,406,480 + Pf6N2E2_4674 0.62 -1.3
5,406,481 - Pf6N2E2_4674 0.62 -0.4
5,406,538 - Pf6N2E2_4674 0.66 -0.3
5,406,855 + Pf6N2E2_4674 0.88 +1.1
5,406,855 + Pf6N2E2_4674 0.88 -0.6
5,406,855 + Pf6N2E2_4674 0.88 -0.1
5,406,856 - Pf6N2E2_4674 0.88 -0.4
5,406,856 - Pf6N2E2_4674 0.88 -0.0
5,407,039 + -1.1
5,407,039 + -1.1
5,407,039 + -0.9
5,407,039 + -0.5
5,407,039 + -0.9
5,407,040 - +0.8
5,407,040 - -0.0
5,407,040 - -0.1
5,407,040 - +0.1
5,407,040 - -0.2
5,407,040 - -0.7
5,407,040 - -0.7
5,407,064 - +0.2
5,407,208 + -0.4
5,407,223 + +1.0
5,407,223 + -0.0
5,407,223 + -0.0
5,407,223 + +0.1
5,407,224 - -0.8
5,407,224 - +0.2
5,407,224 - -0.1
5,407,224 - +1.2
5,407,224 - +0.4
5,407,292 + +0.8
5,407,292 + +0.9
5,407,293 - +0.3
5,407,293 - +0.4
5,407,361 + Pf6N2E2_4675 0.10 -0.1
5,407,476 - Pf6N2E2_4675 0.19 +0.6
5,407,502 + Pf6N2E2_4675 0.21 -0.6
5,407,503 - Pf6N2E2_4675 0.21 +0.2
5,407,503 - Pf6N2E2_4675 0.21 +0.4
5,407,503 - Pf6N2E2_4675 0.21 +0.2
5,407,503 - Pf6N2E2_4675 0.21 -2.3
5,407,532 + Pf6N2E2_4675 0.23 -0.9
5,407,532 + Pf6N2E2_4675 0.23 +0.1
5,407,532 + Pf6N2E2_4675 0.23 -0.4
5,407,532 + Pf6N2E2_4675 0.23 +0.1
5,407,532 + Pf6N2E2_4675 0.23 +0.3
5,407,533 - Pf6N2E2_4675 0.23 +0.7
5,407,533 - Pf6N2E2_4675 0.23 -0.2
5,407,533 - Pf6N2E2_4675 0.23 -0.1
5,407,533 - Pf6N2E2_4675 0.23 -0.0
5,407,533 - Pf6N2E2_4675 0.23 +0.2
5,407,533 - Pf6N2E2_4675 0.23 -0.3
5,407,533 - Pf6N2E2_4675 0.23 -0.3
5,407,533 - Pf6N2E2_4675 0.23 -0.9
5,407,533 - Pf6N2E2_4675 0.23 -0.2
5,407,533 - Pf6N2E2_4675 0.23 +0.4
5,407,533 - Pf6N2E2_4675 0.23 +0.6
5,407,583 + Pf6N2E2_4675 0.27 -0.6
5,407,583 + Pf6N2E2_4675 0.27 +0.6
5,407,583 + Pf6N2E2_4675 0.27 -0.8
5,407,584 - Pf6N2E2_4675 0.27 -0.8
5,407,584 - Pf6N2E2_4675 0.27 -0.4
5,407,584 - Pf6N2E2_4675 0.27 -0.2
5,407,880 + Pf6N2E2_4675 0.49 +0.6
5,407,880 + Pf6N2E2_4675 0.49 +0.2
5,407,881 - Pf6N2E2_4675 0.49 +0.5
5,407,998 - Pf6N2E2_4675 0.58 +0.8
5,407,998 - Pf6N2E2_4675 0.58 +1.1
5,408,123 + +1.1
5,408,123 + Pf6N2E2_4675 0.67 -0.4
5,408,206 + Pf6N2E2_4675 0.74 +0.8
5,408,206 + Pf6N2E2_4675 0.74 +0.2
5,408,206 + Pf6N2E2_4675 0.74 -1.1
5,408,206 + Pf6N2E2_4675 0.74 -0.3
5,408,206 + Pf6N2E2_4675 0.74 +0.3
5,408,207 - Pf6N2E2_4675 0.74 -0.1
5,408,207 - Pf6N2E2_4675 0.74 +0.3
5,408,729 + Pf6N2E2_4676 0.12 +0.2
5,408,737 + Pf6N2E2_4676 0.12 +0.0
5,408,738 - Pf6N2E2_4676 0.12 -1.2
5,408,857 - Pf6N2E2_4676 0.21 +0.3
5,409,091 + Pf6N2E2_4676 0.37 +0.5
5,409,091 + Pf6N2E2_4676 0.37 +0.3
5,409,092 - Pf6N2E2_4676 0.37 -0.6
5,409,092 - Pf6N2E2_4676 0.37 -0.1
5,409,200 - Pf6N2E2_4676 0.44 +0.4
5,409,485 - Pf6N2E2_4676 0.64 -0.4
5,409,532 + Pf6N2E2_4676 0.67 -0.2
5,409,533 - Pf6N2E2_4676 0.67 -0.3

Or see this region's nucleotide sequence