Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4652 and Pf6N2E2_4653 are separated by 19 nucleotides Pf6N2E2_4653 and Pf6N2E2_4654 are separated by 31 nucleotides Pf6N2E2_4654 and Pf6N2E2_4655 are separated by 39 nucleotides
Pf6N2E2_4652 - S-adenosylmethionine synthetase (EC 2.5.1.6), at 5,381,926 to 5,383,116
_4652
Pf6N2E2_4653 - Transcriptional regulator, ArsR family / Methyltransferase fusion, at 5,383,136 to 5,384,131
_4653
Pf6N2E2_4654 - hypothetical protein, at 5,384,163 to 5,384,384
_4654
Pf6N2E2_4655 - Transketolase (EC 2.2.1.1), at 5,384,424 to 5,386,421
_4655
Position (kb)
5383
5384
5385 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 5383.203 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.209 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.213 kb on - strand at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.332 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.431 kb on - strand, within Pf6N2E2_4653 at 5383.493 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.650 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5383.779 kb on - strand, within Pf6N2E2_4653 at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.040 kb on - strand at 5384.124 kb on - strand at 5384.124 kb on - strand at 5384.132 kb on - strand at 5384.152 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.165 kb on - strand at 5384.222 kb on - strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.252 kb on + strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.253 kb on - strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.295 kb on + strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.296 kb on - strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.324 kb on + strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.325 kb on - strand, within Pf6N2E2_4654 at 5384.387 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 5,383,203 - +0.5 5,383,209 - +0.3 5,383,209 - +0.6 5,383,209 - +0.1 5,383,213 - +0.8 5,383,213 - -0.4 5,383,213 - +0.1 5,383,213 - +0.3 5,383,213 - +0.6 5,383,213 - -1.2 5,383,332 - Pf6N2E2_4653 0.20 +0.2 5,383,332 - Pf6N2E2_4653 0.20 +1.2 5,383,332 - Pf6N2E2_4653 0.20 +0.8 5,383,332 - Pf6N2E2_4653 0.20 -0.7 5,383,332 - Pf6N2E2_4653 0.20 +0.4 5,383,332 - Pf6N2E2_4653 0.20 -1.4 5,383,431 - Pf6N2E2_4653 0.30 +0.8 5,383,431 - Pf6N2E2_4653 0.30 +0.5 5,383,493 - Pf6N2E2_4653 0.36 +1.0 5,383,650 - Pf6N2E2_4653 0.52 +1.1 5,383,650 - Pf6N2E2_4653 0.52 +0.1 5,383,650 - Pf6N2E2_4653 0.52 +0.0 5,383,650 - Pf6N2E2_4653 0.52 +1.3 5,383,650 - Pf6N2E2_4653 0.52 +1.4 5,383,779 - Pf6N2E2_4653 0.65 +0.3 5,383,779 - Pf6N2E2_4653 0.65 +0.6 5,383,779 - Pf6N2E2_4653 0.65 -0.6 5,384,040 - -0.0 5,384,040 - +0.8 5,384,040 - +0.4 5,384,124 - -0.9 5,384,124 - +1.3 5,384,132 - +0.6 5,384,152 - -2.8 5,384,165 - +1.0 5,384,165 - +0.7 5,384,165 - +0.0 5,384,222 - Pf6N2E2_4654 0.27 +1.4 5,384,252 + Pf6N2E2_4654 0.40 -1.1 5,384,252 + Pf6N2E2_4654 0.40 -0.4 5,384,253 - Pf6N2E2_4654 0.41 +1.7 5,384,253 - Pf6N2E2_4654 0.41 +0.7 5,384,253 - Pf6N2E2_4654 0.41 +0.9 5,384,253 - Pf6N2E2_4654 0.41 +0.5 5,384,253 - Pf6N2E2_4654 0.41 +1.6 5,384,253 - Pf6N2E2_4654 0.41 +1.4 5,384,253 - Pf6N2E2_4654 0.41 +1.3 5,384,253 - Pf6N2E2_4654 0.41 +1.1 5,384,295 + Pf6N2E2_4654 0.59 +1.5 5,384,295 + Pf6N2E2_4654 0.59 -0.7 5,384,295 + Pf6N2E2_4654 0.59 -0.9 5,384,295 + Pf6N2E2_4654 0.59 -0.6 5,384,296 - Pf6N2E2_4654 0.60 +3.0 5,384,296 - Pf6N2E2_4654 0.60 +3.0 5,384,296 - Pf6N2E2_4654 0.60 +1.8 5,384,324 + Pf6N2E2_4654 0.73 -0.6 5,384,324 + Pf6N2E2_4654 0.73 -0.2 5,384,324 + Pf6N2E2_4654 0.73 +0.5 5,384,325 - Pf6N2E2_4654 0.73 +3.9 5,384,325 - Pf6N2E2_4654 0.73 +3.8 5,384,387 + +1.0
Or see this region's nucleotide sequence