Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4586 and Pf6N2E2_4587 are separated by 185 nucleotides Pf6N2E2_4587 and Pf6N2E2_4588 are separated by 73 nucleotides Pf6N2E2_4588 and Pf6N2E2_4589 are separated by 79 nucleotides Pf6N2E2_4589 and Pf6N2E2_4590 are separated by 54 nucleotides
Pf6N2E2_4586 - RNA polymerase sigma factor RpoH, at 5,316,529 to 5,317,383
_4586
Pf6N2E2_4587 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 5,317,569 to 5,318,291
_4587
Pf6N2E2_4588 - COG2363, at 5,318,365 to 5,318,745
_4588
Pf6N2E2_4589 - Sulfur carrier protein ThiS, at 5,318,825 to 5,319,043
_4589
Pf6N2E2_4590 - Thiazole biosynthesis protein ThiG, at 5,319,098 to 5,319,892
_4590
Position (kb)
5317
5318
5319 Strain fitness (log2 ratio)
-2
-1
0
1 at 5317.400 kb on + strand at 5317.557 kb on + strand at 5317.558 kb on - strand at 5317.615 kb on + strand at 5317.616 kb on - strand at 5317.675 kb on + strand, within Pf6N2E2_4587 at 5317.675 kb on + strand, within Pf6N2E2_4587 at 5317.676 kb on - strand, within Pf6N2E2_4587 at 5317.837 kb on + strand, within Pf6N2E2_4587 at 5317.837 kb on + strand, within Pf6N2E2_4587 at 5317.837 kb on + strand, within Pf6N2E2_4587 at 5317.837 kb on + strand, within Pf6N2E2_4587 at 5317.838 kb on - strand, within Pf6N2E2_4587 at 5317.838 kb on - strand, within Pf6N2E2_4587 at 5317.838 kb on - strand, within Pf6N2E2_4587 at 5317.838 kb on - strand, within Pf6N2E2_4587 at 5318.280 kb on + strand at 5318.281 kb on - strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.291 kb on + strand at 5318.292 kb on - strand at 5318.327 kb on + strand at 5318.328 kb on - strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.329 kb on + strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.330 kb on - strand at 5318.373 kb on + strand at 5318.373 kb on + strand at 5318.374 kb on - strand at 5318.374 kb on - strand at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.467 kb on + strand, within Pf6N2E2_4588 at 5318.468 kb on - strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.497 kb on + strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.498 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.512 kb on - strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.603 kb on + strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.604 kb on - strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.623 kb on + strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.624 kb on - strand, within Pf6N2E2_4588 at 5318.759 kb on + strand at 5318.759 kb on + strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.760 kb on - strand at 5318.805 kb on + strand at 5318.805 kb on + strand at 5319.142 kb on + strand at 5319.142 kb on + strand at 5319.143 kb on - strand at 5319.180 kb on - strand, within Pf6N2E2_4590
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 5,317,400 + -1.8 5,317,557 + -0.7 5,317,558 - +0.9 5,317,615 + -1.0 5,317,616 - -0.0 5,317,675 + Pf6N2E2_4587 0.15 -0.2 5,317,675 + Pf6N2E2_4587 0.15 +0.7 5,317,676 - Pf6N2E2_4587 0.15 +0.2 5,317,837 + Pf6N2E2_4587 0.37 -1.1 5,317,837 + Pf6N2E2_4587 0.37 -1.0 5,317,837 + Pf6N2E2_4587 0.37 -1.0 5,317,837 + Pf6N2E2_4587 0.37 +0.7 5,317,838 - Pf6N2E2_4587 0.37 +1.0 5,317,838 - Pf6N2E2_4587 0.37 +0.1 5,317,838 - Pf6N2E2_4587 0.37 -1.7 5,317,838 - Pf6N2E2_4587 0.37 +0.2 5,318,280 + -0.5 5,318,281 - -0.8 5,318,291 + -0.4 5,318,291 + -1.1 5,318,291 + -0.7 5,318,291 + -0.7 5,318,291 + +0.4 5,318,292 - +0.0 5,318,327 + +0.2 5,318,328 - +0.0 5,318,329 + +0.5 5,318,329 + +0.4 5,318,329 + -0.7 5,318,329 + +0.7 5,318,329 + -0.3 5,318,329 + -0.2 5,318,329 + -0.3 5,318,329 + +0.2 5,318,329 + +0.3 5,318,329 + -0.5 5,318,329 + +0.5 5,318,329 + -0.8 5,318,329 + +0.8 5,318,330 - +0.6 5,318,330 - -0.9 5,318,330 - +1.1 5,318,330 - +0.8 5,318,330 - +1.0 5,318,330 - +1.4 5,318,330 - -0.4 5,318,330 - -0.9 5,318,330 - +0.4 5,318,330 - +0.4 5,318,373 + -0.7 5,318,373 + +1.0 5,318,374 - +0.1 5,318,374 - -1.4 5,318,467 + Pf6N2E2_4588 0.27 +0.4 5,318,467 + Pf6N2E2_4588 0.27 +0.3 5,318,468 - Pf6N2E2_4588 0.27 -0.4 5,318,497 + Pf6N2E2_4588 0.35 +0.5 5,318,497 + Pf6N2E2_4588 0.35 -1.0 5,318,497 + Pf6N2E2_4588 0.35 +0.2 5,318,497 + Pf6N2E2_4588 0.35 +0.2 5,318,497 + Pf6N2E2_4588 0.35 +1.0 5,318,498 - Pf6N2E2_4588 0.35 -0.4 5,318,498 - Pf6N2E2_4588 0.35 +0.1 5,318,498 - Pf6N2E2_4588 0.35 -0.4 5,318,498 - Pf6N2E2_4588 0.35 -0.6 5,318,498 - Pf6N2E2_4588 0.35 -0.2 5,318,512 - Pf6N2E2_4588 0.39 -0.7 5,318,512 - Pf6N2E2_4588 0.39 +0.8 5,318,603 + Pf6N2E2_4588 0.62 -0.8 5,318,603 + Pf6N2E2_4588 0.62 +0.9 5,318,603 + Pf6N2E2_4588 0.62 -0.2 5,318,603 + Pf6N2E2_4588 0.62 -1.1 5,318,603 + Pf6N2E2_4588 0.62 -0.3 5,318,603 + Pf6N2E2_4588 0.62 +0.0 5,318,603 + Pf6N2E2_4588 0.62 +0.7 5,318,603 + Pf6N2E2_4588 0.62 -1.5 5,318,603 + Pf6N2E2_4588 0.62 -0.7 5,318,603 + Pf6N2E2_4588 0.62 +0.1 5,318,603 + Pf6N2E2_4588 0.62 -1.0 5,318,604 - Pf6N2E2_4588 0.63 +0.9 5,318,604 - Pf6N2E2_4588 0.63 -0.4 5,318,604 - Pf6N2E2_4588 0.63 +0.6 5,318,604 - Pf6N2E2_4588 0.63 -0.7 5,318,604 - Pf6N2E2_4588 0.63 -0.2 5,318,604 - Pf6N2E2_4588 0.63 +0.6 5,318,604 - Pf6N2E2_4588 0.63 +1.0 5,318,604 - Pf6N2E2_4588 0.63 -0.7 5,318,604 - Pf6N2E2_4588 0.63 +0.8 5,318,604 - Pf6N2E2_4588 0.63 -0.6 5,318,604 - Pf6N2E2_4588 0.63 +0.2 5,318,604 - Pf6N2E2_4588 0.63 +0.3 5,318,604 - Pf6N2E2_4588 0.63 +1.0 5,318,623 + Pf6N2E2_4588 0.68 +0.1 5,318,623 + Pf6N2E2_4588 0.68 -1.7 5,318,623 + Pf6N2E2_4588 0.68 -0.3 5,318,624 - Pf6N2E2_4588 0.68 +0.1 5,318,624 - Pf6N2E2_4588 0.68 -0.1 5,318,624 - Pf6N2E2_4588 0.68 -0.8 5,318,759 + +0.0 5,318,759 + -2.1 5,318,760 - +0.0 5,318,760 - -2.4 5,318,760 - +0.1 5,318,805 + +0.2 5,318,805 + +0.9 5,319,142 + -0.4 5,319,142 + -0.2 5,319,143 - +0.1 5,319,180 - Pf6N2E2_4590 0.10 +1.1
Or see this region's nucleotide sequence