Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4217 and Pf6N2E2_4218 are separated by 316 nucleotides Pf6N2E2_4218 and Pf6N2E2_4219 overlap by 4 nucleotides Pf6N2E2_4219 and Pf6N2E2_4220 overlap by 4 nucleotides Pf6N2E2_4220 and Pf6N2E2_4221 are separated by 15 nucleotides
Pf6N2E2_4217 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 4,907,997 to 4,909,829
_4217
Pf6N2E2_4218 - hypothetical protein, at 4,910,146 to 4,910,574
_4218
Pf6N2E2_4219 - hypothetical protein, at 4,910,571 to 4,910,864
_4219
Pf6N2E2_4220 - RNA polymerase sigma factor RpoE, at 4,910,861 to 4,911,436
_4220
Pf6N2E2_4221 - hypothetical protein, at 4,911,452 to 4,911,775
_4221
Position (kb)
4910
4911 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4909.870 kb on + strand at 4909.977 kb on + strand at 4909.978 kb on - strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.979 kb on + strand at 4909.980 kb on - strand at 4909.984 kb on + strand at 4909.984 kb on + strand at 4909.984 kb on + strand at 4909.985 kb on - strand at 4909.985 kb on - strand at 4909.985 kb on - strand at 4910.043 kb on - strand at 4910.163 kb on + strand at 4910.164 kb on - strand at 4910.164 kb on - strand at 4910.164 kb on - strand at 4910.189 kb on - strand, within Pf6N2E2_4218 at 4910.201 kb on - strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.214 kb on + strand, within Pf6N2E2_4218 at 4910.275 kb on + strand, within Pf6N2E2_4218 at 4910.275 kb on + strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.276 kb on - strand, within Pf6N2E2_4218 at 4910.313 kb on + strand, within Pf6N2E2_4218 at 4910.314 kb on - strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.317 kb on + strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.318 kb on - strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.320 kb on + strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.321 kb on - strand, within Pf6N2E2_4218 at 4910.425 kb on + strand, within Pf6N2E2_4218 at 4910.426 kb on - strand, within Pf6N2E2_4218 at 4910.498 kb on - strand, within Pf6N2E2_4218 at 4910.498 kb on - strand, within Pf6N2E2_4218 at 4910.574 kb on + strand at 4910.574 kb on + strand at 4910.574 kb on + strand at 4910.575 kb on - strand at 4910.575 kb on - strand at 4910.640 kb on - strand, within Pf6N2E2_4219 at 4910.915 kb on + strand at 4910.915 kb on + strand at 4910.915 kb on + strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4910.916 kb on - strand at 4911.009 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.169 kb on + strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.170 kb on - strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.208 kb on + strand, within Pf6N2E2_4220 at 4911.209 kb on - strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.223 kb on + strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.224 kb on - strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.342 kb on + strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.343 kb on - strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.376 kb on + strand, within Pf6N2E2_4220 at 4911.377 kb on - strand, within Pf6N2E2_4220 at 4911.377 kb on - strand, within Pf6N2E2_4220 at 4911.385 kb on - strand at 4911.413 kb on + strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.414 kb on - strand at 4911.453 kb on + strand at 4911.453 kb on + strand at 4911.454 kb on - strand at 4911.454 kb on - strand at 4911.529 kb on + strand, within Pf6N2E2_4221 at 4911.530 kb on - strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.543 kb on + strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.544 kb on - strand, within Pf6N2E2_4221 at 4911.549 kb on + strand, within Pf6N2E2_4221 at 4911.549 kb on + strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221 at 4911.550 kb on - strand, within Pf6N2E2_4221
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 4,909,870 + +0.7 4,909,977 + +0.6 4,909,978 - -0.5 4,909,979 + +1.6 4,909,979 + -0.3 4,909,979 + +0.7 4,909,979 + +0.2 4,909,980 - +0.4 4,909,984 + +0.5 4,909,984 + +0.5 4,909,984 + +0.9 4,909,985 - -0.7 4,909,985 - -0.5 4,909,985 - -0.6 4,910,043 - +0.0 4,910,163 + -1.4 4,910,164 - -1.2 4,910,164 - +0.3 4,910,164 - -0.7 4,910,189 - Pf6N2E2_4218 0.10 -1.9 4,910,201 - Pf6N2E2_4218 0.13 -0.6 4,910,214 + Pf6N2E2_4218 0.16 -3.0 4,910,214 + Pf6N2E2_4218 0.16 -1.8 4,910,214 + Pf6N2E2_4218 0.16 -2.3 4,910,214 + Pf6N2E2_4218 0.16 -0.0 4,910,275 + Pf6N2E2_4218 0.30 -0.9 4,910,275 + Pf6N2E2_4218 0.30 -1.6 4,910,276 - Pf6N2E2_4218 0.30 -1.8 4,910,276 - Pf6N2E2_4218 0.30 -1.4 4,910,276 - Pf6N2E2_4218 0.30 -1.2 4,910,276 - Pf6N2E2_4218 0.30 -1.9 4,910,313 + Pf6N2E2_4218 0.39 -2.0 4,910,314 - Pf6N2E2_4218 0.39 -4.1 4,910,317 + Pf6N2E2_4218 0.40 +1.6 4,910,317 + Pf6N2E2_4218 0.40 +0.1 4,910,317 + Pf6N2E2_4218 0.40 -1.6 4,910,317 + Pf6N2E2_4218 0.40 -1.6 4,910,317 + Pf6N2E2_4218 0.40 -1.7 4,910,318 - Pf6N2E2_4218 0.40 -0.0 4,910,318 - Pf6N2E2_4218 0.40 -0.5 4,910,318 - Pf6N2E2_4218 0.40 -3.5 4,910,318 - Pf6N2E2_4218 0.40 -1.5 4,910,318 - Pf6N2E2_4218 0.40 -1.0 4,910,318 - Pf6N2E2_4218 0.40 -1.4 4,910,320 + Pf6N2E2_4218 0.41 -1.6 4,910,320 + Pf6N2E2_4218 0.41 -2.4 4,910,320 + Pf6N2E2_4218 0.41 -2.0 4,910,321 - Pf6N2E2_4218 0.41 -2.3 4,910,321 - Pf6N2E2_4218 0.41 -0.7 4,910,321 - Pf6N2E2_4218 0.41 -1.5 4,910,321 - Pf6N2E2_4218 0.41 -2.2 4,910,425 + Pf6N2E2_4218 0.65 +0.1 4,910,426 - Pf6N2E2_4218 0.65 -0.8 4,910,498 - Pf6N2E2_4218 0.82 -0.6 4,910,498 - Pf6N2E2_4218 0.82 -1.0 4,910,574 + -1.8 4,910,574 + -2.3 4,910,574 + -0.4 4,910,575 - -0.9 4,910,575 - -2.2 4,910,640 - Pf6N2E2_4219 0.23 -3.5 4,910,915 + +0.1 4,910,915 + +0.3 4,910,915 + -0.7 4,910,916 - +0.7 4,910,916 - -0.0 4,910,916 - +0.3 4,910,916 - -0.1 4,911,009 + Pf6N2E2_4220 0.26 -0.4 4,911,169 + Pf6N2E2_4220 0.53 +0.2 4,911,169 + Pf6N2E2_4220 0.53 +0.1 4,911,169 + Pf6N2E2_4220 0.53 -0.7 4,911,169 + Pf6N2E2_4220 0.53 +0.1 4,911,170 - Pf6N2E2_4220 0.54 -0.4 4,911,170 - Pf6N2E2_4220 0.54 -0.3 4,911,170 - Pf6N2E2_4220 0.54 -0.7 4,911,170 - Pf6N2E2_4220 0.54 +0.6 4,911,170 - Pf6N2E2_4220 0.54 +0.6 4,911,170 - Pf6N2E2_4220 0.54 +0.4 4,911,170 - Pf6N2E2_4220 0.54 +0.1 4,911,208 + Pf6N2E2_4220 0.60 -0.3 4,911,208 + Pf6N2E2_4220 0.60 -0.2 4,911,208 + Pf6N2E2_4220 0.60 +0.1 4,911,209 - Pf6N2E2_4220 0.60 +0.8 4,911,223 + Pf6N2E2_4220 0.63 -0.8 4,911,223 + Pf6N2E2_4220 0.63 +0.2 4,911,223 + Pf6N2E2_4220 0.63 +1.0 4,911,223 + Pf6N2E2_4220 0.63 +3.3 4,911,224 - Pf6N2E2_4220 0.63 -1.4 4,911,224 - Pf6N2E2_4220 0.63 +0.4 4,911,224 - Pf6N2E2_4220 0.63 -0.3 4,911,224 - Pf6N2E2_4220 0.63 +0.1 4,911,224 - Pf6N2E2_4220 0.63 +0.5 4,911,224 - Pf6N2E2_4220 0.63 +0.5 4,911,224 - Pf6N2E2_4220 0.63 +1.0 4,911,224 - Pf6N2E2_4220 0.63 -0.3 4,911,224 - Pf6N2E2_4220 0.63 +0.5 4,911,224 - Pf6N2E2_4220 0.63 -0.4 4,911,224 - Pf6N2E2_4220 0.63 +1.1 4,911,224 - Pf6N2E2_4220 0.63 -1.1 4,911,224 - Pf6N2E2_4220 0.63 +0.1 4,911,224 - Pf6N2E2_4220 0.63 +1.5 4,911,342 + Pf6N2E2_4220 0.84 -0.2 4,911,342 + Pf6N2E2_4220 0.84 -0.8 4,911,342 + Pf6N2E2_4220 0.84 +0.5 4,911,342 + Pf6N2E2_4220 0.84 -1.0 4,911,342 + Pf6N2E2_4220 0.84 +0.4 4,911,342 + Pf6N2E2_4220 0.84 -0.2 4,911,342 + Pf6N2E2_4220 0.84 -1.0 4,911,342 + Pf6N2E2_4220 0.84 -0.3 4,911,342 + Pf6N2E2_4220 0.84 -1.1 4,911,342 + Pf6N2E2_4220 0.84 +0.5 4,911,342 + Pf6N2E2_4220 0.84 +0.2 4,911,343 - Pf6N2E2_4220 0.84 +0.1 4,911,343 - Pf6N2E2_4220 0.84 -0.8 4,911,343 - Pf6N2E2_4220 0.84 -0.6 4,911,343 - Pf6N2E2_4220 0.84 -0.4 4,911,343 - Pf6N2E2_4220 0.84 -0.4 4,911,343 - Pf6N2E2_4220 0.84 -0.3 4,911,376 + Pf6N2E2_4220 0.89 +0.0 4,911,376 + Pf6N2E2_4220 0.89 +0.8 4,911,376 + Pf6N2E2_4220 0.89 -0.0 4,911,377 - Pf6N2E2_4220 0.90 +0.3 4,911,377 - Pf6N2E2_4220 0.90 -0.6 4,911,385 - +1.5 4,911,413 + +1.7 4,911,414 - -1.8 4,911,414 - -0.1 4,911,414 - -0.5 4,911,414 - -0.1 4,911,453 + -0.2 4,911,453 + -0.3 4,911,454 - -0.0 4,911,454 - +0.0 4,911,529 + Pf6N2E2_4221 0.24 -0.6 4,911,530 - Pf6N2E2_4221 0.24 +0.0 4,911,543 + Pf6N2E2_4221 0.28 -0.0 4,911,543 + Pf6N2E2_4221 0.28 +0.0 4,911,543 + Pf6N2E2_4221 0.28 -0.2 4,911,543 + Pf6N2E2_4221 0.28 -0.5 4,911,543 + Pf6N2E2_4221 0.28 -0.9 4,911,543 + Pf6N2E2_4221 0.28 -0.1 4,911,543 + Pf6N2E2_4221 0.28 -0.5 4,911,543 + Pf6N2E2_4221 0.28 -0.2 4,911,543 + Pf6N2E2_4221 0.28 -1.3 4,911,544 - Pf6N2E2_4221 0.28 +0.9 4,911,544 - Pf6N2E2_4221 0.28 -1.3 4,911,544 - Pf6N2E2_4221 0.28 -0.0 4,911,544 - Pf6N2E2_4221 0.28 -0.1 4,911,544 - Pf6N2E2_4221 0.28 -0.8 4,911,544 - Pf6N2E2_4221 0.28 -0.1 4,911,544 - Pf6N2E2_4221 0.28 +0.3 4,911,544 - Pf6N2E2_4221 0.28 -0.0 4,911,549 + Pf6N2E2_4221 0.30 -2.3 4,911,549 + Pf6N2E2_4221 0.30 -0.2 4,911,550 - Pf6N2E2_4221 0.30 +0.6 4,911,550 - Pf6N2E2_4221 0.30 -0.7 4,911,550 - Pf6N2E2_4221 0.30 +0.1 4,911,550 - Pf6N2E2_4221 0.30 +0.4
Or see this region's nucleotide sequence