Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4213 and Pf6N2E2_4214 are separated by 45 nucleotides Pf6N2E2_4214 and Pf6N2E2_4215 are separated by 115 nucleotides Pf6N2E2_4215 and Pf6N2E2_4216 are separated by 209 nucleotides Pf6N2E2_4216 and Pf6N2E2_4217 are separated by 1 nucleotides
Pf6N2E2_4213 - ATP synthase beta chain (EC 3.6.3.14), at 4,903,679 to 4,905,055
_4213
Pf6N2E2_4214 - ATP synthase epsilon chain (EC 3.6.3.14), at 4,905,101 to 4,905,526
_4214
Pf6N2E2_4215 - N-acetylglucosamine-1-phosphate uridyltransferase (EC 2.7.7.23) / Glucosamine-1-phosphate N-acetyltransferase (EC 2.3.1.157), at 4,905,642 to 4,907,009
_4215
Pf6N2E2_4216 - Transcriptional regulator of glmS gene, DeoR family, at 4,907,219 to 4,907,995
_4216
Pf6N2E2_4217 - Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (EC 2.6.1.16), at 4,907,997 to 4,909,829
_4217
Position (kb)
4905
4906
4907
4908 Strain fitness (log2 ratio)
-1
0
1 at 4907.013 kb on + strand at 4907.059 kb on + strand at 4907.067 kb on - strand at 4907.708 kb on + strand, within Pf6N2E2_4216 at 4907.761 kb on + strand, within Pf6N2E2_4216 at 4907.761 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.823 kb on + strand, within Pf6N2E2_4216 at 4907.959 kb on + strand at 4907.959 kb on + strand at 4907.959 kb on + strand at 4907.959 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 4,907,013 + +0.4 4,907,059 + +0.6 4,907,067 - -0.0 4,907,708 + Pf6N2E2_4216 0.63 +0.2 4,907,761 + Pf6N2E2_4216 0.70 +0.7 4,907,761 + Pf6N2E2_4216 0.70 -1.7 4,907,823 + Pf6N2E2_4216 0.78 -0.5 4,907,823 + Pf6N2E2_4216 0.78 -1.5 4,907,823 + Pf6N2E2_4216 0.78 +0.5 4,907,959 + -0.2 4,907,959 + +0.2 4,907,959 + +0.7 4,907,959 + +0.5
Or see this region's nucleotide sequence