Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3492 and Pf6N2E2_3493 are separated by 194 nucleotides Pf6N2E2_3493 and Pf6N2E2_3494 are separated by 2 nucleotides Pf6N2E2_3494 and Pf6N2E2_3495 are separated by 92 nucleotides
Pf6N2E2_3492 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 4,073,333 to 4,074,610
_3492
Pf6N2E2_3493 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 4,074,805 to 4,076,157
_3493
Pf6N2E2_3494 - Acyl dehydratase, at 4,076,160 to 4,077,014
_3494
Pf6N2E2_3495 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 4,077,107 to 4,077,661
_3495
Position (kb)
4074
4075
4076
4077 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 4074.350 kb on + strand, within Pf6N2E2_3492 at 4074.357 kb on + strand, within Pf6N2E2_3492 at 4074.358 kb on - strand, within Pf6N2E2_3492 at 4074.358 kb on - strand, within Pf6N2E2_3492 at 4074.480 kb on + strand, within Pf6N2E2_3492 at 4074.481 kb on - strand, within Pf6N2E2_3492 at 4074.481 kb on - strand, within Pf6N2E2_3492 at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.540 kb on + strand at 4074.541 kb on - strand at 4074.541 kb on - strand at 4074.599 kb on + strand at 4074.599 kb on + strand at 4074.599 kb on + strand at 4074.600 kb on - strand at 4074.600 kb on - strand at 4074.600 kb on - strand at 4074.627 kb on + strand at 4074.627 kb on + strand at 4074.627 kb on + strand at 4074.628 kb on - strand at 4074.633 kb on + strand at 4074.633 kb on + strand at 4074.633 kb on + strand at 4074.641 kb on + strand at 4074.641 kb on + strand at 4074.642 kb on - strand at 4074.726 kb on + strand at 4074.726 kb on + strand at 4074.726 kb on + strand at 4074.727 kb on - strand at 4074.733 kb on + strand at 4074.733 kb on + strand at 4074.734 kb on - strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.817 kb on + strand at 4074.820 kb on - strand at 4074.939 kb on - strand at 4075.009 kb on + strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.010 kb on - strand, within Pf6N2E2_3493 at 4075.078 kb on + strand, within Pf6N2E2_3493 at 4075.300 kb on + strand, within Pf6N2E2_3493 at 4075.300 kb on + strand, within Pf6N2E2_3493 at 4075.301 kb on - strand, within Pf6N2E2_3493 at 4075.605 kb on + strand, within Pf6N2E2_3493 at 4075.606 kb on - strand, within Pf6N2E2_3493 at 4075.870 kb on + strand, within Pf6N2E2_3493 at 4076.129 kb on + strand at 4076.130 kb on - strand at 4076.130 kb on - strand at 4076.212 kb on - strand at 4076.461 kb on + strand, within Pf6N2E2_3494 at 4076.461 kb on + strand, within Pf6N2E2_3494 at 4076.462 kb on - strand, within Pf6N2E2_3494 at 4076.718 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.739 kb on + strand, within Pf6N2E2_3494 at 4076.740 kb on - strand, within Pf6N2E2_3494 at 4076.740 kb on - strand, within Pf6N2E2_3494 at 4076.984 kb on + strand at 4076.984 kb on + strand at 4076.984 kb on + strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.985 kb on - strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4076.989 kb on + strand at 4077.120 kb on + strand at 4077.121 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 4,074,350 + Pf6N2E2_3492 0.80 +0.4 4,074,357 + Pf6N2E2_3492 0.80 +0.6 4,074,358 - Pf6N2E2_3492 0.80 +0.9 4,074,358 - Pf6N2E2_3492 0.80 -0.0 4,074,480 + Pf6N2E2_3492 0.90 +0.0 4,074,481 - Pf6N2E2_3492 0.90 -0.2 4,074,481 - Pf6N2E2_3492 0.90 +0.8 4,074,540 + -1.9 4,074,540 + -2.5 4,074,540 + -0.7 4,074,540 + +0.4 4,074,541 - +0.5 4,074,541 - +0.9 4,074,599 + -0.8 4,074,599 + +0.7 4,074,599 + +1.2 4,074,600 - -0.1 4,074,600 - +0.1 4,074,600 - +0.2 4,074,627 + -0.2 4,074,627 + -0.3 4,074,627 + +0.4 4,074,628 - -1.2 4,074,633 + -0.3 4,074,633 + -0.3 4,074,633 + +0.4 4,074,641 + -0.5 4,074,641 + +2.4 4,074,642 - -0.7 4,074,726 + -0.4 4,074,726 + +0.1 4,074,726 + -0.1 4,074,727 - -4.2 4,074,733 + +0.5 4,074,733 + -1.3 4,074,734 - -2.8 4,074,817 + -1.0 4,074,817 + +0.1 4,074,817 + +0.1 4,074,817 + +0.0 4,074,817 + +0.7 4,074,817 + +0.3 4,074,820 - -2.0 4,074,939 - -1.8 4,075,009 + Pf6N2E2_3493 0.15 -0.6 4,075,010 - Pf6N2E2_3493 0.15 -3.4 4,075,010 - Pf6N2E2_3493 0.15 -3.5 4,075,010 - Pf6N2E2_3493 0.15 +0.2 4,075,010 - Pf6N2E2_3493 0.15 -3.4 4,075,078 + Pf6N2E2_3493 0.20 -1.1 4,075,300 + Pf6N2E2_3493 0.37 -0.1 4,075,300 + Pf6N2E2_3493 0.37 -1.3 4,075,301 - Pf6N2E2_3493 0.37 -2.6 4,075,605 + Pf6N2E2_3493 0.59 -1.8 4,075,606 - Pf6N2E2_3493 0.59 -2.4 4,075,870 + Pf6N2E2_3493 0.79 -0.7 4,076,129 + +0.2 4,076,130 - -2.5 4,076,130 - -2.5 4,076,212 - -0.7 4,076,461 + Pf6N2E2_3494 0.35 +0.5 4,076,461 + Pf6N2E2_3494 0.35 +0.8 4,076,462 - Pf6N2E2_3494 0.35 -0.5 4,076,718 + Pf6N2E2_3494 0.65 -0.2 4,076,739 + Pf6N2E2_3494 0.68 -0.2 4,076,739 + Pf6N2E2_3494 0.68 +0.4 4,076,739 + Pf6N2E2_3494 0.68 -0.2 4,076,740 - Pf6N2E2_3494 0.68 -0.4 4,076,740 - Pf6N2E2_3494 0.68 -0.3 4,076,984 + +0.1 4,076,984 + +0.3 4,076,984 + +0.7 4,076,985 - -0.9 4,076,985 - -0.4 4,076,985 - -1.4 4,076,985 - -1.3 4,076,985 - +0.4 4,076,985 - -0.1 4,076,985 - -1.9 4,076,985 - +0.2 4,076,989 + -0.2 4,076,989 + -0.5 4,076,989 + -1.1 4,076,989 + -0.8 4,077,120 + +0.2 4,077,121 - +0.2
Or see this region's nucleotide sequence