Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2941 and Pf6N2E2_2942 are separated by 271 nucleotides Pf6N2E2_2942 and Pf6N2E2_2943 are separated by 98 nucleotides Pf6N2E2_2943 and Pf6N2E2_2944 are separated by 63 nucleotides
Pf6N2E2_2941 - Esterase A, at 3,432,878 to 3,434,023
_2941
Pf6N2E2_2942 - ATP-dependent helicase DinG/Rad3, at 3,434,295 to 3,436,439
_2942
Pf6N2E2_2943 - Phosphoribosylcarboxyaminoimidazole (NCAIR) mutase, at 3,436,538 to 3,437,002
_2943
Pf6N2E2_2944 - Collagen pro alpha-chain precursor, at 3,437,066 to 3,437,824
_2944
Position (kb)
3434
3435
3436
3437 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3433.542 kb on + strand, within Pf6N2E2_2941 at 3433.543 kb on - strand, within Pf6N2E2_2941 at 3433.557 kb on + strand, within Pf6N2E2_2941 at 3433.558 kb on - strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3433.629 kb on + strand, within Pf6N2E2_2941 at 3434.004 kb on + strand at 3434.004 kb on + strand at 3434.573 kb on - strand, within Pf6N2E2_2942 at 3435.010 kb on + strand, within Pf6N2E2_2942 at 3435.176 kb on + strand, within Pf6N2E2_2942 at 3435.176 kb on + strand, within Pf6N2E2_2942 at 3435.177 kb on - strand, within Pf6N2E2_2942 at 3435.215 kb on - strand, within Pf6N2E2_2942 at 3435.610 kb on + strand, within Pf6N2E2_2942 at 3435.610 kb on + strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.611 kb on - strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.667 kb on + strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.668 kb on - strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.784 kb on + strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.785 kb on - strand, within Pf6N2E2_2942 at 3435.833 kb on + strand, within Pf6N2E2_2942 at 3435.908 kb on + strand, within Pf6N2E2_2942 at 3435.909 kb on - strand, within Pf6N2E2_2942 at 3435.909 kb on - strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.060 kb on + strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.061 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.136 kb on - strand, within Pf6N2E2_2942 at 3436.225 kb on + strand at 3436.399 kb on + strand at 3436.499 kb on + strand at 3436.499 kb on + strand at 3436.500 kb on - strand at 3436.500 kb on - strand at 3436.559 kb on + strand at 3436.560 kb on - strand at 3436.560 kb on - strand at 3436.560 kb on - strand at 3436.573 kb on - strand at 3436.573 kb on - strand at 3436.678 kb on - strand, within Pf6N2E2_2943 at 3436.743 kb on + strand, within Pf6N2E2_2943 at 3436.744 kb on - strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.790 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.814 kb on + strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.815 kb on - strand, within Pf6N2E2_2943 at 3436.873 kb on + strand, within Pf6N2E2_2943 at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.011 kb on + strand at 3437.012 kb on - strand at 3437.012 kb on - strand at 3437.160 kb on + strand, within Pf6N2E2_2944 at 3437.160 kb on + strand, within Pf6N2E2_2944 at 3437.266 kb on - strand, within Pf6N2E2_2944
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 3,433,542 + Pf6N2E2_2941 0.58 -0.8 3,433,543 - Pf6N2E2_2941 0.58 +2.1 3,433,557 + Pf6N2E2_2941 0.59 -1.9 3,433,558 - Pf6N2E2_2941 0.59 -0.2 3,433,629 + Pf6N2E2_2941 0.66 -1.4 3,433,629 + Pf6N2E2_2941 0.66 -2.6 3,433,629 + Pf6N2E2_2941 0.66 -0.0 3,433,629 + Pf6N2E2_2941 0.66 -2.3 3,434,004 + -2.9 3,434,004 + -0.4 3,434,573 - Pf6N2E2_2942 0.13 +1.1 3,435,010 + Pf6N2E2_2942 0.33 -1.7 3,435,176 + Pf6N2E2_2942 0.41 -2.7 3,435,176 + Pf6N2E2_2942 0.41 -2.3 3,435,177 - Pf6N2E2_2942 0.41 -1.2 3,435,215 - Pf6N2E2_2942 0.43 -0.0 3,435,610 + Pf6N2E2_2942 0.61 -1.1 3,435,610 + Pf6N2E2_2942 0.61 -1.4 3,435,611 - Pf6N2E2_2942 0.61 -1.8 3,435,611 - Pf6N2E2_2942 0.61 -0.1 3,435,611 - Pf6N2E2_2942 0.61 +0.4 3,435,667 + Pf6N2E2_2942 0.64 -0.9 3,435,667 + Pf6N2E2_2942 0.64 -2.3 3,435,667 + Pf6N2E2_2942 0.64 -1.0 3,435,667 + Pf6N2E2_2942 0.64 -1.0 3,435,667 + Pf6N2E2_2942 0.64 -1.8 3,435,667 + Pf6N2E2_2942 0.64 -3.5 3,435,667 + Pf6N2E2_2942 0.64 -1.3 3,435,667 + Pf6N2E2_2942 0.64 -1.8 3,435,667 + Pf6N2E2_2942 0.64 -3.5 3,435,667 + Pf6N2E2_2942 0.64 -4.4 3,435,668 - Pf6N2E2_2942 0.64 -3.0 3,435,668 - Pf6N2E2_2942 0.64 -1.5 3,435,668 - Pf6N2E2_2942 0.64 -2.5 3,435,668 - Pf6N2E2_2942 0.64 -0.3 3,435,668 - Pf6N2E2_2942 0.64 -1.3 3,435,668 - Pf6N2E2_2942 0.64 -1.9 3,435,784 + Pf6N2E2_2942 0.69 -2.8 3,435,784 + Pf6N2E2_2942 0.69 -1.2 3,435,784 + Pf6N2E2_2942 0.69 -0.6 3,435,784 + Pf6N2E2_2942 0.69 -1.2 3,435,784 + Pf6N2E2_2942 0.69 -1.6 3,435,785 - Pf6N2E2_2942 0.69 +0.1 3,435,785 - Pf6N2E2_2942 0.69 -2.7 3,435,785 - Pf6N2E2_2942 0.69 -1.7 3,435,833 + Pf6N2E2_2942 0.72 -0.6 3,435,908 + Pf6N2E2_2942 0.75 -2.3 3,435,909 - Pf6N2E2_2942 0.75 -2.6 3,435,909 - Pf6N2E2_2942 0.75 -0.3 3,436,060 + Pf6N2E2_2942 0.82 +0.2 3,436,060 + Pf6N2E2_2942 0.82 -1.5 3,436,060 + Pf6N2E2_2942 0.82 -2.9 3,436,061 - Pf6N2E2_2942 0.82 -3.0 3,436,061 - Pf6N2E2_2942 0.82 -2.0 3,436,061 - Pf6N2E2_2942 0.82 -0.5 3,436,136 - Pf6N2E2_2942 0.86 -2.0 3,436,136 - Pf6N2E2_2942 0.86 -1.5 3,436,136 - Pf6N2E2_2942 0.86 -1.3 3,436,225 + -1.6 3,436,399 + -1.0 3,436,499 + -0.1 3,436,499 + -0.1 3,436,500 - -1.4 3,436,500 - -0.4 3,436,559 + -1.6 3,436,560 - +0.5 3,436,560 - +0.3 3,436,560 - +1.6 3,436,573 - -1.8 3,436,573 - +0.1 3,436,678 - Pf6N2E2_2943 0.30 -0.8 3,436,743 + Pf6N2E2_2943 0.44 -0.5 3,436,744 - Pf6N2E2_2943 0.44 +1.0 3,436,790 + Pf6N2E2_2943 0.54 -2.7 3,436,790 + Pf6N2E2_2943 0.54 +0.2 3,436,790 + Pf6N2E2_2943 0.54 -0.4 3,436,814 + Pf6N2E2_2943 0.59 +0.4 3,436,814 + Pf6N2E2_2943 0.59 -0.0 3,436,814 + Pf6N2E2_2943 0.59 -0.4 3,436,814 + Pf6N2E2_2943 0.59 -0.1 3,436,814 + Pf6N2E2_2943 0.59 +0.5 3,436,814 + Pf6N2E2_2943 0.59 +1.1 3,436,814 + Pf6N2E2_2943 0.59 -0.0 3,436,814 + Pf6N2E2_2943 0.59 +1.4 3,436,815 - Pf6N2E2_2943 0.60 +0.3 3,436,815 - Pf6N2E2_2943 0.60 +1.1 3,436,815 - Pf6N2E2_2943 0.60 -0.9 3,436,815 - Pf6N2E2_2943 0.60 +0.1 3,436,815 - Pf6N2E2_2943 0.60 -0.5 3,436,815 - Pf6N2E2_2943 0.60 -0.4 3,436,815 - Pf6N2E2_2943 0.60 +0.4 3,436,815 - Pf6N2E2_2943 0.60 +0.4 3,436,815 - Pf6N2E2_2943 0.60 -0.2 3,436,873 + Pf6N2E2_2943 0.72 -0.3 3,437,011 + -0.1 3,437,011 + -1.1 3,437,011 + -1.0 3,437,011 + -0.0 3,437,011 + +1.0 3,437,011 + +0.1 3,437,012 - +0.3 3,437,012 - -1.1 3,437,160 + Pf6N2E2_2944 0.12 -0.1 3,437,160 + Pf6N2E2_2944 0.12 +0.6 3,437,266 - Pf6N2E2_2944 0.26 +0.7
Or see this region's nucleotide sequence