Experiment: Lomefloxacin 0.0002 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2522 and Pf6N2E2_2523 are separated by 441 nucleotides Pf6N2E2_2523 and Pf6N2E2_2524 are separated by 136 nucleotides
Pf6N2E2_2522 - DNA gyrase subunit A (EC 5.99.1.3), at 2,976,737 to 2,979,400
_2522
Pf6N2E2_2523 - Methylthioribose-1-phosphate isomerase (EC 5.3.1.23), at 2,979,842 to 2,980,888
_2523
Pf6N2E2_2524 - S-adenosylhomocysteine deaminase (EC 3.5.4.28); Methylthioadenosine deaminase, at 2,981,025 to 2,982,356
_2524
Position (kb)
2979
2980
2981 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2979.621 kb on + strand at 2979.621 kb on + strand at 2979.622 kb on - strand at 2979.622 kb on - strand at 2979.625 kb on + strand at 2979.625 kb on + strand at 2979.626 kb on - strand at 2979.626 kb on - strand at 2979.665 kb on + strand at 2979.665 kb on + strand at 2979.666 kb on - strand at 2979.666 kb on - strand at 2979.666 kb on - strand at 2979.666 kb on - strand at 2979.666 kb on - strand at 2979.759 kb on + strand at 2979.760 kb on - strand at 2979.760 kb on - strand at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.137 kb on + strand, within Pf6N2E2_2523 at 2980.138 kb on - strand, within Pf6N2E2_2523 at 2980.138 kb on - strand, within Pf6N2E2_2523 at 2980.138 kb on - strand, within Pf6N2E2_2523 at 2980.138 kb on - strand, within Pf6N2E2_2523 at 2980.138 kb on - strand, within Pf6N2E2_2523 at 2980.141 kb on + strand, within Pf6N2E2_2523 at 2980.142 kb on - strand, within Pf6N2E2_2523 at 2980.332 kb on + strand, within Pf6N2E2_2523 at 2980.333 kb on - strand, within Pf6N2E2_2523 at 2980.358 kb on - strand, within Pf6N2E2_2523 at 2980.359 kb on + strand, within Pf6N2E2_2523 at 2980.359 kb on + strand, within Pf6N2E2_2523 at 2980.359 kb on + strand, within Pf6N2E2_2523 at 2980.359 kb on + strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.360 kb on - strand, within Pf6N2E2_2523 at 2980.725 kb on + strand, within Pf6N2E2_2523 at 2980.725 kb on + strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.726 kb on - strand, within Pf6N2E2_2523 at 2980.854 kb on + strand at 2980.855 kb on - strand at 2980.855 kb on - strand at 2980.954 kb on + strand at 2980.955 kb on - strand at 2980.955 kb on - strand at 2980.956 kb on + strand at 2980.957 kb on - strand at 2980.957 kb on - strand at 2980.984 kb on + strand at 2980.986 kb on + strand at 2981.001 kb on + strand at 2981.001 kb on + strand at 2981.001 kb on + strand at 2981.003 kb on + strand at 2981.053 kb on + strand at 2981.053 kb on + strand at 2981.053 kb on + strand at 2981.053 kb on + strand at 2981.053 kb on + strand at 2981.053 kb on + strand at 2981.056 kb on + strand at 2981.056 kb on + strand at 2981.098 kb on + strand at 2981.098 kb on + strand at 2981.353 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.421 kb on + strand, within Pf6N2E2_2524 at 2981.427 kb on + strand, within Pf6N2E2_2524 at 2981.615 kb on + strand, within Pf6N2E2_2524 at 2981.681 kb on + strand, within Pf6N2E2_2524 at 2981.681 kb on + strand, within Pf6N2E2_2524 at 2981.681 kb on + strand, within Pf6N2E2_2524 at 2981.682 kb on - strand, within Pf6N2E2_2524 at 2981.682 kb on - strand, within Pf6N2E2_2524
Per-strain Table
Position Strand Gene LocusTag Fraction Lomefloxacin 0.0002 mM remove 2,979,621 + +2.7 2,979,621 + +1.9 2,979,622 - -0.8 2,979,622 - +0.2 2,979,625 + +3.3 2,979,625 + +2.8 2,979,626 - -2.0 2,979,626 - -1.9 2,979,665 + +2.3 2,979,665 + +3.2 2,979,666 - -0.5 2,979,666 - -0.8 2,979,666 - +2.4 2,979,666 - -0.2 2,979,666 - -3.4 2,979,759 + +2.2 2,979,760 - +0.4 2,979,760 - -1.4 2,980,137 + Pf6N2E2_2523 0.28 -1.8 2,980,137 + Pf6N2E2_2523 0.28 +0.2 2,980,137 + Pf6N2E2_2523 0.28 +0.9 2,980,137 + Pf6N2E2_2523 0.28 -0.5 2,980,137 + Pf6N2E2_2523 0.28 -0.8 2,980,137 + Pf6N2E2_2523 0.28 -0.8 2,980,137 + Pf6N2E2_2523 0.28 -2.9 2,980,137 + Pf6N2E2_2523 0.28 -0.9 2,980,137 + Pf6N2E2_2523 0.28 -1.5 2,980,137 + Pf6N2E2_2523 0.28 +0.6 2,980,138 - Pf6N2E2_2523 0.28 +0.3 2,980,138 - Pf6N2E2_2523 0.28 -1.6 2,980,138 - Pf6N2E2_2523 0.28 -1.3 2,980,138 - Pf6N2E2_2523 0.28 -1.8 2,980,138 - Pf6N2E2_2523 0.28 -1.8 2,980,141 + Pf6N2E2_2523 0.29 -0.2 2,980,142 - Pf6N2E2_2523 0.29 -0.0 2,980,332 + Pf6N2E2_2523 0.47 -0.9 2,980,333 - Pf6N2E2_2523 0.47 -1.1 2,980,358 - Pf6N2E2_2523 0.49 -1.4 2,980,359 + Pf6N2E2_2523 0.49 -0.9 2,980,359 + Pf6N2E2_2523 0.49 -0.4 2,980,359 + Pf6N2E2_2523 0.49 -0.8 2,980,359 + Pf6N2E2_2523 0.49 -1.8 2,980,360 - Pf6N2E2_2523 0.49 -0.9 2,980,360 - Pf6N2E2_2523 0.49 -1.8 2,980,360 - Pf6N2E2_2523 0.49 -0.7 2,980,360 - Pf6N2E2_2523 0.49 -1.8 2,980,360 - Pf6N2E2_2523 0.49 -1.5 2,980,360 - Pf6N2E2_2523 0.49 -1.0 2,980,360 - Pf6N2E2_2523 0.49 -1.1 2,980,725 + Pf6N2E2_2523 0.84 -0.9 2,980,725 + Pf6N2E2_2523 0.84 -0.1 2,980,726 - Pf6N2E2_2523 0.84 -0.2 2,980,726 - Pf6N2E2_2523 0.84 -0.8 2,980,726 - Pf6N2E2_2523 0.84 -2.2 2,980,726 - Pf6N2E2_2523 0.84 -3.3 2,980,726 - Pf6N2E2_2523 0.84 -2.7 2,980,726 - Pf6N2E2_2523 0.84 -1.0 2,980,854 + -1.3 2,980,855 - -1.4 2,980,855 - -2.8 2,980,954 + -0.9 2,980,955 - +0.7 2,980,955 - +1.5 2,980,956 + +1.5 2,980,957 - +0.9 2,980,957 - +0.1 2,980,984 + +2.6 2,980,986 + +0.8 2,981,001 + -0.3 2,981,001 + +0.6 2,981,001 + +1.1 2,981,003 + -0.2 2,981,053 + -0.1 2,981,053 + -0.8 2,981,053 + +0.3 2,981,053 + +1.2 2,981,053 + +0.3 2,981,053 + +0.8 2,981,056 + +0.8 2,981,056 + +0.4 2,981,098 + +0.1 2,981,098 + -0.5 2,981,353 + Pf6N2E2_2524 0.25 -0.1 2,981,421 + Pf6N2E2_2524 0.30 +0.5 2,981,421 + Pf6N2E2_2524 0.30 +0.2 2,981,421 + Pf6N2E2_2524 0.30 +1.1 2,981,421 + Pf6N2E2_2524 0.30 +1.1 2,981,421 + Pf6N2E2_2524 0.30 -0.1 2,981,421 + Pf6N2E2_2524 0.30 -0.6 2,981,421 + Pf6N2E2_2524 0.30 -1.3 2,981,421 + Pf6N2E2_2524 0.30 +1.2 2,981,421 + Pf6N2E2_2524 0.30 +0.4 2,981,421 + Pf6N2E2_2524 0.30 +1.1 2,981,421 + Pf6N2E2_2524 0.30 +0.4 2,981,421 + Pf6N2E2_2524 0.30 -1.5 2,981,427 + Pf6N2E2_2524 0.30 -0.3 2,981,615 + Pf6N2E2_2524 0.44 -0.3 2,981,681 + Pf6N2E2_2524 0.49 -0.1 2,981,681 + Pf6N2E2_2524 0.49 +0.6 2,981,681 + Pf6N2E2_2524 0.49 +1.5 2,981,682 - Pf6N2E2_2524 0.49 +1.2 2,981,682 - Pf6N2E2_2524 0.49 -1.5
Or see this region's nucleotide sequence