Experiment: D-Glucose (C) with ammonium chloride; anaerobic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS13440 and HSERO_RS13445 overlap by 13 nucleotides HSERO_RS13445 and HSERO_RS13450 are separated by 5 nucleotides HSERO_RS13450 and HSERO_RS13455 are separated by 10 nucleotides HSERO_RS13455 and HSERO_RS13460 overlap by 4 nucleotides
HSERO_RS13440: HSERO_RS13440 - 2-(5''-triphosphoribosyl)-3'-dephospho-CoA synthase, at 3,057,892 to 3,058,830
_RS13440
HSERO_RS13445: HSERO_RS13445 - phosphoribosyl-dephospho-CoA transferase, at 3,058,818 to 3,059,531
_RS13445
HSERO_RS13450: HSERO_RS13450 - malonate decarboxylase subunit gamma, at 3,059,537 to 3,060,277
_RS13450
HSERO_RS13455: HSERO_RS13455 - acetyl-CoA carboxylase subunit beta, at 3,060,288 to 3,061,184
_RS13455
HSERO_RS13460: HSERO_RS13460 - malonate decarboxylase subunit delta, at 3,061,181 to 3,061,495
_RS13460
Position (kb)
3059
3060
3061 Strain fitness (log2 ratio)
-2
-1
0
1 at 3058.716 kb on + strand, within HSERO_RS13440 at 3058.773 kb on + strand at 3059.505 kb on - strand at 3059.515 kb on - strand at 3059.592 kb on - strand at 3059.874 kb on - strand, within HSERO_RS13450 at 3059.970 kb on + strand, within HSERO_RS13450 at 3059.970 kb on + strand, within HSERO_RS13450 at 3060.284 kb on + strand at 3060.595 kb on + strand, within HSERO_RS13455 at 3061.138 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Glucose (C) with ammonium chloride; anaerobic; 3 days remove 3,058,716 + HSERO_RS13440 0.88 -2.5 3,058,773 + -0.1 3,059,505 - -0.0 3,059,515 - -0.8 3,059,592 - +0.1 3,059,874 - HSERO_RS13450 0.45 +0.2 3,059,970 + HSERO_RS13450 0.58 +0.6 3,059,970 + HSERO_RS13450 0.58 +0.4 3,060,284 + +0.7 3,060,595 + HSERO_RS13455 0.34 +0.5 3,061,138 + -0.1
Or see this region's nucleotide sequence