Experiment: Iron limitation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04525 and MMP_RS04530 are separated by 41 nucleotides MMP_RS04530 and MMP_RS04535 are separated by 120 nucleotides
MMP_RS04525: MMP_RS04525 - D-2-hydroxyacid dehydrogenase, at 861,628 to 862,584
_RS04525
MMP_RS04530: MMP_RS04530 - ABC transporter permease, at 862,626 to 863,819
_RS04530
MMP_RS04535: MMP_RS04535 - hydroxymethylbilane synthase, at 863,940 to 864,854
_RS04535
Position (kb)
862
863
864 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 861.642 kb on + strand at 861.715 kb on - strand at 861.715 kb on - strand at 861.716 kb on + strand at 861.740 kb on - strand, within MMP_RS04525 at 861.774 kb on - strand, within MMP_RS04525 at 861.775 kb on + strand, within MMP_RS04525 at 861.780 kb on - strand, within MMP_RS04525 at 861.781 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.786 kb on + strand, within MMP_RS04525 at 861.825 kb on + strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.829 kb on - strand, within MMP_RS04525 at 861.830 kb on + strand, within MMP_RS04525 at 861.840 kb on - strand, within MMP_RS04525 at 861.853 kb on - strand, within MMP_RS04525 at 861.894 kb on + strand, within MMP_RS04525 at 861.900 kb on + strand, within MMP_RS04525 at 861.945 kb on + strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.956 kb on - strand, within MMP_RS04525 at 861.957 kb on + strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.985 kb on - strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.986 kb on + strand, within MMP_RS04525 at 861.987 kb on - strand, within MMP_RS04525 at 861.999 kb on + strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.145 kb on - strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.146 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.152 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.446 kb on + strand, within MMP_RS04525 at 862.456 kb on + strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.461 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.465 kb on - strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.466 kb on + strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.467 kb on - strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.468 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.470 kb on + strand, within MMP_RS04525 at 862.564 kb on + strand at 862.566 kb on + strand at 862.578 kb on - strand at 862.585 kb on - strand at 862.589 kb on - strand at 862.594 kb on + strand at 862.594 kb on + strand at 862.667 kb on + strand at 862.693 kb on - strand at 862.714 kb on - strand at 862.714 kb on - strand at 862.715 kb on + strand at 862.715 kb on + strand at 862.718 kb on - strand at 862.767 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.771 kb on - strand, within MMP_RS04530 at 862.883 kb on + strand, within MMP_RS04530 at 862.976 kb on + strand, within MMP_RS04530 at 863.006 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.263 kb on + strand, within MMP_RS04530 at 863.267 kb on - strand, within MMP_RS04530 at 863.309 kb on + strand, within MMP_RS04530 at 863.510 kb on - strand, within MMP_RS04530 at 863.728 kb on - strand at 863.740 kb on - strand at 863.740 kb on - strand at 863.740 kb on - strand at 863.752 kb on - strand at 863.753 kb on + strand at 863.753 kb on + strand at 863.760 kb on - strand at 863.801 kb on - strand at 863.802 kb on + strand at 863.836 kb on + strand at 863.847 kb on + strand at 863.849 kb on - strand at 863.874 kb on + strand at 863.915 kb on + strand at 863.920 kb on + strand at 863.924 kb on + strand at 863.969 kb on - strand at 863.970 kb on + strand at 863.994 kb on - strand at 863.995 kb on + strand at 864.010 kb on - strand at 864.011 kb on + strand at 864.047 kb on - strand at 864.048 kb on + strand at 864.075 kb on + strand at 864.245 kb on + strand at 864.290 kb on + strand at 864.424 kb on - strand at 864.425 kb on + strand at 864.700 kb on - strand at 864.729 kb on + strand at 864.763 kb on - strand at 864.766 kb on - strand at 864.767 kb on + strand at 864.771 kb on + strand at 864.771 kb on + strand at 864.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Iron limitation, H2 media remove 861,642 + +0.7 861,715 - -1.9 861,715 - +0.0 861,716 + +1.4 861,740 - MMP_RS04525 0.12 -0.2 861,774 - MMP_RS04525 0.15 +0.6 861,775 + MMP_RS04525 0.15 -0.9 861,780 - MMP_RS04525 0.16 +0.0 861,781 + MMP_RS04525 0.16 -0.1 861,786 + MMP_RS04525 0.17 -0.2 861,786 + MMP_RS04525 0.17 -0.1 861,825 + MMP_RS04525 0.21 -0.3 861,829 - MMP_RS04525 0.21 -0.4 861,829 - MMP_RS04525 0.21 +0.1 861,829 - MMP_RS04525 0.21 -0.6 861,830 + MMP_RS04525 0.21 +0.3 861,840 - MMP_RS04525 0.22 +0.1 861,853 - MMP_RS04525 0.24 -1.2 861,894 + MMP_RS04525 0.28 -0.1 861,900 + MMP_RS04525 0.28 +0.2 861,945 + MMP_RS04525 0.33 -0.4 861,956 - MMP_RS04525 0.34 +0.5 861,956 - MMP_RS04525 0.34 +0.5 861,957 + MMP_RS04525 0.34 +0.1 861,985 - MMP_RS04525 0.37 +0.5 861,985 - MMP_RS04525 0.37 +0.5 861,986 + MMP_RS04525 0.37 +0.2 861,986 + MMP_RS04525 0.37 +0.2 861,987 - MMP_RS04525 0.38 -0.1 861,999 + MMP_RS04525 0.39 -0.5 862,145 - MMP_RS04525 0.54 +0.1 862,145 - MMP_RS04525 0.54 -0.6 862,145 - MMP_RS04525 0.54 +0.2 862,146 + MMP_RS04525 0.54 -0.6 862,146 + MMP_RS04525 0.54 +1.1 862,146 + MMP_RS04525 0.54 -0.5 862,152 + MMP_RS04525 0.55 +0.5 862,152 + MMP_RS04525 0.55 +0.3 862,446 + MMP_RS04525 0.85 -0.3 862,446 + MMP_RS04525 0.85 +0.1 862,456 + MMP_RS04525 0.87 +0.4 862,461 - MMP_RS04525 0.87 +0.4 862,461 - MMP_RS04525 0.87 -0.2 862,465 - MMP_RS04525 0.87 -0.4 862,465 - MMP_RS04525 0.87 +0.2 862,465 - MMP_RS04525 0.87 -0.9 862,465 - MMP_RS04525 0.87 -0.2 862,466 + MMP_RS04525 0.88 +0.6 862,466 + MMP_RS04525 0.88 +0.1 862,466 + MMP_RS04525 0.88 -0.1 862,467 - MMP_RS04525 0.88 -0.1 862,467 - MMP_RS04525 0.88 +0.2 862,467 - MMP_RS04525 0.88 -0.6 862,467 - MMP_RS04525 0.88 +0.7 862,468 + MMP_RS04525 0.88 +0.1 862,468 + MMP_RS04525 0.88 -0.4 862,468 + MMP_RS04525 0.88 +0.1 862,470 + MMP_RS04525 0.88 -1.0 862,470 + MMP_RS04525 0.88 -0.1 862,470 + MMP_RS04525 0.88 -0.2 862,470 + MMP_RS04525 0.88 -0.1 862,564 + +0.2 862,566 + -0.1 862,578 - -0.3 862,585 - -0.9 862,589 - +0.7 862,594 + -0.4 862,594 + +1.0 862,667 + +0.2 862,693 - +0.1 862,714 - +0.3 862,714 - -0.2 862,715 + +0.3 862,715 + -0.4 862,718 - +0.1 862,767 - MMP_RS04530 0.12 +0.7 862,771 - MMP_RS04530 0.12 -0.4 862,771 - MMP_RS04530 0.12 +0.1 862,771 - MMP_RS04530 0.12 +0.2 862,883 + MMP_RS04530 0.22 +0.5 862,976 + MMP_RS04530 0.29 +0.1 863,006 + MMP_RS04530 0.32 +0.1 863,263 + MMP_RS04530 0.53 +0.5 863,263 + MMP_RS04530 0.53 +0.2 863,267 - MMP_RS04530 0.54 +0.1 863,309 + MMP_RS04530 0.57 +0.7 863,510 - MMP_RS04530 0.74 +0.6 863,728 - -0.6 863,740 - +0.4 863,740 - +0.1 863,740 - -0.5 863,752 - +0.4 863,753 + -0.2 863,753 + +0.2 863,760 - +0.3 863,801 - +0.5 863,802 + +0.7 863,836 + +0.5 863,847 + -0.4 863,849 - -0.2 863,874 + +0.4 863,915 + -2.1 863,920 + -0.9 863,924 + -0.2 863,969 - -1.9 863,970 + -0.8 863,994 - -3.1 863,995 + -2.9 864,010 - -2.2 864,011 + -0.9 864,047 - -0.9 864,048 + -1.6 864,075 + -3.0 864,245 + -1.8 864,290 + -1.9 864,424 - -0.7 864,425 + -1.0 864,700 - -1.4 864,729 + -2.4 864,763 - -1.0 864,766 - -4.1 864,767 + -2.4 864,771 + -1.7 864,771 + -2.8 864,771 + -1.9
Or see this region's nucleotide sequence