Experiment: Iron limitation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02210 and MMP_RS02215 are separated by 88 nucleotides MMP_RS02215 and MMP_RS02220 are separated by 207 nucleotides MMP_RS02220 and MMP_RS02225 are separated by 19 nucleotides
MMP_RS02210: MMP_RS02210 - bifunctional N(6)-L-threonylcarbamoyladenine synthase/serine/threonine protein kinase, at 416,131 to 417,777
_RS02210
MMP_RS02215: MMP_RS02215 - ECF transporter S component, at 417,866 to 418,462
_RS02215
MMP_RS02220: MMP_RS02220 - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase, at 418,670 to 419,395
_RS02220
MMP_RS02225: MMP_RS02225 - carbohydrate kinase family protein, at 419,415 to 420,323
_RS02225
Position (kb) 417
418
419 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 417.160 kb on + strand at 417.569 kb on - strand at 417.661 kb on - strand at 417.811 kb on - strand at 417.812 kb on + strand at 417.817 kb on - strand at 417.818 kb on + strand at 417.847 kb on + strand at 417.863 kb on + strand at 417.876 kb on - strand at 417.925 kb on + strand at 417.994 kb on - strand, within MMP_RS02215 at 418.097 kb on - strand, within MMP_RS02215 at 418.098 kb on + strand, within MMP_RS02215 at 418.111 kb on + strand, within MMP_RS02215 at 418.145 kb on + strand, within MMP_RS02215 at 418.194 kb on - strand, within MMP_RS02215 at 418.238 kb on - strand, within MMP_RS02215 at 418.279 kb on - strand, within MMP_RS02215 at 418.281 kb on - strand, within MMP_RS02215 at 418.281 kb on - strand, within MMP_RS02215 at 418.282 kb on + strand, within MMP_RS02215 at 418.290 kb on + strand, within MMP_RS02215 at 418.296 kb on - strand, within MMP_RS02215 at 418.296 kb on - strand, within MMP_RS02215 at 418.296 kb on - strand, within MMP_RS02215 at 418.326 kb on - strand, within MMP_RS02215 at 418.354 kb on + strand, within MMP_RS02215 at 418.381 kb on + strand, within MMP_RS02215 at 418.542 kb on - strand at 418.542 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.582 kb on - strand at 418.586 kb on - strand at 418.586 kb on - strand at 418.625 kb on - strand at 418.674 kb on - strand at 419.176 kb on + strand at 419.325 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Iron limitation, H2 media remove 417,160 + -2.3 417,569 - -2.9 417,661 - -2.4 417,811 - -1.1 417,812 + +0.7 417,817 - +0.1 417,818 + +0.7 417,847 + +0.4 417,863 + +0.1 417,876 - -0.1 417,925 + -0.1 417,994 - MMP_RS02215 0.21 +0.6 418,097 - MMP_RS02215 0.39 +0.8 418,098 + MMP_RS02215 0.39 +0.5 418,111 + MMP_RS02215 0.41 +0.8 418,145 + MMP_RS02215 0.47 +0.2 418,194 - MMP_RS02215 0.55 +0.3 418,238 - MMP_RS02215 0.62 -0.1 418,279 - MMP_RS02215 0.69 +0.5 418,281 - MMP_RS02215 0.70 +0.4 418,281 - MMP_RS02215 0.70 +0.1 418,282 + MMP_RS02215 0.70 +0.8 418,290 + MMP_RS02215 0.71 +0.1 418,296 - MMP_RS02215 0.72 -0.6 418,296 - MMP_RS02215 0.72 +0.2 418,296 - MMP_RS02215 0.72 +0.1 418,326 - MMP_RS02215 0.77 +0.6 418,354 + MMP_RS02215 0.82 +0.2 418,381 + MMP_RS02215 0.86 +0.4 418,542 - -0.2 418,542 - +0.5 418,582 - -0.5 418,582 - -0.9 418,582 - -0.5 418,582 - -0.1 418,582 - -0.2 418,586 - -0.9 418,586 - -3.7 418,625 - -1.6 418,674 - -1.9 419,176 + -2.2 419,325 - -1.8
Or see this region's nucleotide sequence