Experiment: Iron limitation, H2 media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01050 and MMP_RS01055 are separated by 334 nucleotides MMP_RS01055 and MMP_RS01060 are separated by 236 nucleotides MMP_RS01060 and MMP_RS01065 are separated by 199 nucleotides
MMP_RS01050: MMP_RS01050 - tRNA-Leu, at 197,613 to 197,697
_RS01050
MMP_RS01055: MMP_RS01055 - hypothetical protein, at 198,032 to 198,409
_RS01055
MMP_RS01060: MMP_RS01060 - calcium/sodium antiporter, at 198,646 to 199,608
_RS01060
MMP_RS01065: MMP_RS01065 - alpha/beta hydrolase, at 199,808 to 200,512
_RS01065
Position (kb) 198
199
200 Strain fitness (log2 ratio) -1
0
1 at 197.811 kb on - strand at 197.856 kb on - strand at 197.894 kb on - strand at 198.086 kb on - strand, within MMP_RS01055 at 198.086 kb on - strand, within MMP_RS01055 at 198.170 kb on + strand, within MMP_RS01055 at 198.406 kb on + strand at 198.406 kb on + strand at 198.411 kb on - strand at 198.411 kb on - strand at 198.412 kb on + strand at 198.444 kb on - strand at 198.569 kb on - strand at 198.605 kb on - strand at 198.640 kb on + strand at 198.644 kb on - strand at 198.820 kb on + strand, within MMP_RS01060 at 198.820 kb on + strand, within MMP_RS01060 at 198.903 kb on - strand, within MMP_RS01060 at 198.914 kb on + strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.930 kb on - strand, within MMP_RS01060 at 198.931 kb on + strand, within MMP_RS01060 at 198.959 kb on + strand, within MMP_RS01060 at 199.182 kb on - strand, within MMP_RS01060 at 199.182 kb on - strand, within MMP_RS01060 at 199.184 kb on - strand, within MMP_RS01060 at 199.184 kb on - strand, within MMP_RS01060 at 199.185 kb on + strand, within MMP_RS01060 at 199.186 kb on - strand, within MMP_RS01060 at 199.189 kb on - strand, within MMP_RS01060 at 199.197 kb on - strand, within MMP_RS01060 at 199.216 kb on - strand, within MMP_RS01060 at 199.283 kb on - strand, within MMP_RS01060 at 199.318 kb on + strand, within MMP_RS01060 at 199.345 kb on + strand, within MMP_RS01060 at 199.345 kb on + strand, within MMP_RS01060 at 199.540 kb on + strand at 199.564 kb on - strand at 199.586 kb on - strand at 199.586 kb on - strand at 199.586 kb on - strand at 199.587 kb on + strand at 199.587 kb on + strand at 199.608 kb on - strand at 199.608 kb on - strand at 199.655 kb on - strand at 199.656 kb on + strand at 199.661 kb on - strand at 199.662 kb on + strand at 199.663 kb on - strand at 199.664 kb on + strand at 199.670 kb on - strand at 199.694 kb on - strand at 199.708 kb on - strand at 199.708 kb on - strand at 199.708 kb on - strand at 199.721 kb on + strand at 199.721 kb on + strand at 199.782 kb on + strand at 199.848 kb on + strand at 199.862 kb on + strand at 199.869 kb on - strand at 199.938 kb on - strand, within MMP_RS01065 at 199.946 kb on - strand, within MMP_RS01065 at 199.946 kb on - strand, within MMP_RS01065 at 199.972 kb on - strand, within MMP_RS01065 at 199.972 kb on - strand, within MMP_RS01065 at 199.972 kb on - strand, within MMP_RS01065 at 199.973 kb on + strand, within MMP_RS01065 at 199.997 kb on + strand, within MMP_RS01065 at 200.037 kb on + strand, within MMP_RS01065 at 200.037 kb on + strand, within MMP_RS01065 at 200.037 kb on + strand, within MMP_RS01065 at 200.060 kb on - strand, within MMP_RS01065 at 200.093 kb on - strand, within MMP_RS01065 at 200.094 kb on + strand, within MMP_RS01065 at 200.156 kb on - strand, within MMP_RS01065 at 200.189 kb on - strand, within MMP_RS01065 at 200.189 kb on - strand, within MMP_RS01065 at 200.197 kb on - strand, within MMP_RS01065 at 200.202 kb on - strand, within MMP_RS01065 at 200.212 kb on - strand, within MMP_RS01065 at 200.256 kb on - strand, within MMP_RS01065 at 200.257 kb on + strand, within MMP_RS01065 at 200.283 kb on + strand, within MMP_RS01065 at 200.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Iron limitation, H2 media remove 197,811 - -0.9 197,856 - -0.3 197,894 - -0.1 198,086 - MMP_RS01055 0.14 +0.5 198,086 - MMP_RS01055 0.14 +1.1 198,170 + MMP_RS01055 0.37 +0.1 198,406 + -0.3 198,406 + +0.3 198,411 - -0.2 198,411 - +0.5 198,412 + +0.2 198,444 - +0.2 198,569 - +0.1 198,605 - -1.1 198,640 + +0.9 198,644 - +0.5 198,820 + MMP_RS01060 0.18 +0.7 198,820 + MMP_RS01060 0.18 +0.9 198,903 - MMP_RS01060 0.27 +0.4 198,914 + MMP_RS01060 0.28 +0.1 198,930 - MMP_RS01060 0.29 +0.8 198,930 - MMP_RS01060 0.29 +0.1 198,930 - MMP_RS01060 0.29 +1.1 198,931 + MMP_RS01060 0.30 -0.2 198,959 + MMP_RS01060 0.33 +0.8 199,182 - MMP_RS01060 0.56 +0.5 199,182 - MMP_RS01060 0.56 +0.0 199,184 - MMP_RS01060 0.56 +0.1 199,184 - MMP_RS01060 0.56 +0.6 199,185 + MMP_RS01060 0.56 +0.6 199,186 - MMP_RS01060 0.56 +0.1 199,189 - MMP_RS01060 0.56 +0.3 199,197 - MMP_RS01060 0.57 +0.2 199,216 - MMP_RS01060 0.59 -0.0 199,283 - MMP_RS01060 0.66 +0.7 199,318 + MMP_RS01060 0.70 -0.5 199,345 + MMP_RS01060 0.73 +0.5 199,345 + MMP_RS01060 0.73 -0.1 199,540 + -0.5 199,564 - +0.3 199,586 - +0.9 199,586 - -0.8 199,586 - -1.2 199,587 + +0.8 199,587 + +0.8 199,608 - +0.2 199,608 - +0.2 199,655 - +0.7 199,656 + -1.6 199,661 - -0.0 199,662 + +0.4 199,663 - -0.5 199,664 + +0.1 199,670 - -0.5 199,694 - -0.2 199,708 - +1.1 199,708 - +0.7 199,708 - -0.2 199,721 + +0.7 199,721 + +0.9 199,782 + +0.2 199,848 + +0.9 199,862 + +0.7 199,869 - -0.4 199,938 - MMP_RS01065 0.18 +0.7 199,946 - MMP_RS01065 0.20 +0.8 199,946 - MMP_RS01065 0.20 +0.3 199,972 - MMP_RS01065 0.23 -0.5 199,972 - MMP_RS01065 0.23 -0.5 199,972 - MMP_RS01065 0.23 +0.3 199,973 + MMP_RS01065 0.23 +0.2 199,997 + MMP_RS01065 0.27 -0.4 200,037 + MMP_RS01065 0.32 +0.5 200,037 + MMP_RS01065 0.32 -0.1 200,037 + MMP_RS01065 0.32 +0.3 200,060 - MMP_RS01065 0.36 -0.1 200,093 - MMP_RS01065 0.40 +0.1 200,094 + MMP_RS01065 0.41 +0.2 200,156 - MMP_RS01065 0.49 -0.0 200,189 - MMP_RS01065 0.54 +1.0 200,189 - MMP_RS01065 0.54 +0.9 200,197 - MMP_RS01065 0.55 +0.6 200,202 - MMP_RS01065 0.56 -0.1 200,212 - MMP_RS01065 0.57 +1.3 200,256 - MMP_RS01065 0.64 +0.5 200,257 + MMP_RS01065 0.64 +0.4 200,283 + MMP_RS01065 0.67 -0.5 200,549 - +0.5
Or see this region's nucleotide sequence