Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_09520 and IAI47_09525 are separated by 56 nucleotides IAI47_09525 and IAI47_09530 are separated by 295 nucleotides IAI47_09530 and IAI47_09535 are separated by 159 nucleotides
IAI47_09520: IAI47_09520 - ATP-dependent RNA helicase HrpA, at 2,008,927 to 2,012,829
_09520
IAI47_09525: IAI47_09525 - serine protease, at 2,012,886 to 2,013,695
_09525
IAI47_09530: IAI47_09530 - acid resistance repetitive basic protein Asr, at 2,013,991 to 2,014,521
_09530
IAI47_09535: IAI47_09535 - carboxypeptidase M32, at 2,014,681 to 2,016,168
_09535
Position (kb)
2012
2013
2014 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2011.920 kb on + strand, within IAI47_09520 at 2011.920 kb on + strand, within IAI47_09520 at 2011.920 kb on + strand, within IAI47_09520 at 2011.921 kb on - strand, within IAI47_09520 at 2012.011 kb on + strand, within IAI47_09520 at 2012.011 kb on + strand, within IAI47_09520 at 2012.011 kb on + strand, within IAI47_09520 at 2012.011 kb on + strand, within IAI47_09520 at 2012.012 kb on - strand, within IAI47_09520 at 2012.012 kb on - strand, within IAI47_09520 at 2012.032 kb on + strand, within IAI47_09520 at 2012.032 kb on + strand, within IAI47_09520 at 2012.033 kb on - strand, within IAI47_09520 at 2012.033 kb on - strand, within IAI47_09520 at 2012.033 kb on - strand, within IAI47_09520 at 2012.172 kb on + strand, within IAI47_09520 at 2012.176 kb on + strand, within IAI47_09520 at 2012.177 kb on - strand, within IAI47_09520 at 2012.177 kb on - strand, within IAI47_09520 at 2012.218 kb on + strand, within IAI47_09520 at 2012.218 kb on + strand, within IAI47_09520 at 2012.218 kb on + strand, within IAI47_09520 at 2012.219 kb on - strand, within IAI47_09520 at 2012.224 kb on - strand, within IAI47_09520 at 2012.231 kb on - strand, within IAI47_09520 at 2012.262 kb on + strand, within IAI47_09520 at 2012.262 kb on + strand, within IAI47_09520 at 2012.262 kb on + strand, within IAI47_09520 at 2012.263 kb on - strand, within IAI47_09520 at 2012.263 kb on - strand, within IAI47_09520 at 2012.280 kb on + strand, within IAI47_09520 at 2012.280 kb on + strand, within IAI47_09520 at 2012.280 kb on + strand, within IAI47_09520 at 2012.281 kb on - strand, within IAI47_09520 at 2012.288 kb on + strand, within IAI47_09520 at 2012.288 kb on + strand, within IAI47_09520 at 2012.288 kb on + strand, within IAI47_09520 at 2012.289 kb on - strand, within IAI47_09520 at 2012.427 kb on + strand, within IAI47_09520 at 2012.427 kb on + strand, within IAI47_09520 at 2012.427 kb on + strand, within IAI47_09520 at 2012.427 kb on + strand, within IAI47_09520 at 2012.428 kb on - strand, within IAI47_09520 at 2012.451 kb on + strand at 2012.451 kb on + strand at 2012.451 kb on + strand at 2012.451 kb on + strand at 2012.451 kb on + strand at 2012.452 kb on - strand at 2012.452 kb on - strand at 2012.452 kb on - strand at 2012.452 kb on - strand at 2012.492 kb on + strand at 2012.492 kb on + strand at 2012.492 kb on + strand at 2012.493 kb on - strand at 2012.493 kb on - strand at 2012.550 kb on + strand at 2012.571 kb on + strand at 2012.572 kb on - strand at 2012.572 kb on - strand at 2012.572 kb on - strand at 2012.572 kb on - strand at 2012.631 kb on - strand at 2012.670 kb on + strand at 2012.670 kb on + strand at 2012.670 kb on + strand at 2012.673 kb on + strand at 2012.674 kb on - strand at 2012.738 kb on + strand at 2012.738 kb on + strand at 2012.738 kb on + strand at 2012.738 kb on + strand at 2012.738 kb on + strand at 2012.738 kb on + strand at 2012.739 kb on - strand at 2012.739 kb on - strand at 2012.739 kb on - strand at 2012.739 kb on - strand at 2012.739 kb on - strand at 2012.742 kb on + strand at 2012.742 kb on + strand at 2012.742 kb on + strand at 2012.743 kb on - strand at 2012.743 kb on - strand at 2012.750 kb on - strand at 2012.765 kb on + strand at 2012.776 kb on + strand at 2012.776 kb on + strand at 2012.776 kb on + strand at 2012.790 kb on + strand at 2012.790 kb on + strand at 2012.820 kb on - strand at 2012.850 kb on - strand at 2012.870 kb on + strand at 2012.871 kb on - strand at 2012.916 kb on - strand at 2012.954 kb on - strand at 2012.994 kb on + strand, within IAI47_09525 at 2013.009 kb on - strand, within IAI47_09525 at 2013.118 kb on + strand, within IAI47_09525 at 2013.126 kb on - strand, within IAI47_09525 at 2013.164 kb on + strand, within IAI47_09525 at 2013.164 kb on + strand, within IAI47_09525 at 2013.165 kb on - strand, within IAI47_09525 at 2013.252 kb on + strand, within IAI47_09525 at 2013.252 kb on + strand, within IAI47_09525 at 2013.252 kb on + strand, within IAI47_09525 at 2013.253 kb on - strand, within IAI47_09525 at 2013.253 kb on - strand, within IAI47_09525 at 2013.255 kb on + strand, within IAI47_09525 at 2013.256 kb on - strand, within IAI47_09525 at 2013.256 kb on - strand, within IAI47_09525 at 2013.256 kb on - strand, within IAI47_09525 at 2013.274 kb on + strand, within IAI47_09525 at 2013.275 kb on - strand, within IAI47_09525 at 2013.275 kb on - strand, within IAI47_09525 at 2013.359 kb on + strand, within IAI47_09525 at 2013.360 kb on - strand, within IAI47_09525 at 2013.360 kb on - strand, within IAI47_09525 at 2013.360 kb on - strand, within IAI47_09525 at 2013.360 kb on - strand, within IAI47_09525 at 2013.495 kb on - strand, within IAI47_09525 at 2013.503 kb on + strand, within IAI47_09525 at 2013.503 kb on + strand, within IAI47_09525 at 2013.551 kb on + strand, within IAI47_09525 at 2013.552 kb on - strand, within IAI47_09525 at 2013.552 kb on - strand, within IAI47_09525 at 2013.552 kb on - strand, within IAI47_09525 at 2013.599 kb on + strand, within IAI47_09525 at 2013.599 kb on + strand, within IAI47_09525 at 2013.599 kb on + strand, within IAI47_09525 at 2013.599 kb on + strand, within IAI47_09525 at 2013.600 kb on - strand, within IAI47_09525 at 2013.614 kb on + strand, within IAI47_09525 at 2013.675 kb on + strand at 2013.683 kb on + strand at 2013.695 kb on + strand at 2013.696 kb on - strand at 2013.727 kb on + strand at 2013.727 kb on + strand at 2013.727 kb on + strand at 2013.733 kb on + strand at 2013.733 kb on + strand at 2013.733 kb on + strand at 2013.736 kb on + strand at 2013.736 kb on + strand at 2013.736 kb on + strand at 2013.736 kb on + strand at 2013.736 kb on + strand at 2013.737 kb on - strand at 2013.740 kb on + strand at 2013.740 kb on + strand at 2013.740 kb on + strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.741 kb on - strand at 2013.744 kb on + strand at 2013.744 kb on + strand at 2013.744 kb on + strand at 2013.749 kb on + strand at 2013.749 kb on + strand at 2013.750 kb on - strand at 2013.753 kb on + strand at 2013.753 kb on + strand at 2013.753 kb on + strand at 2013.753 kb on + strand at 2013.754 kb on - strand at 2013.754 kb on - strand at 2013.754 kb on - strand at 2013.754 kb on - strand at 2013.754 kb on - strand at 2013.755 kb on + strand at 2013.756 kb on - strand at 2013.792 kb on - strand at 2013.804 kb on - strand at 2013.804 kb on - strand at 2013.816 kb on + strand at 2013.852 kb on + strand at 2013.852 kb on + strand at 2013.852 kb on + strand at 2013.853 kb on - strand at 2013.864 kb on + strand at 2013.889 kb on + strand at 2013.889 kb on + strand at 2013.890 kb on - strand at 2013.890 kb on - strand at 2013.956 kb on - strand at 2013.956 kb on - strand at 2013.993 kb on - strand at 2014.038 kb on + strand at 2014.039 kb on - strand at 2014.068 kb on + strand, within IAI47_09530 at 2014.068 kb on + strand, within IAI47_09530 at 2014.069 kb on - strand, within IAI47_09530 at 2014.069 kb on - strand, within IAI47_09530 at 2014.072 kb on + strand, within IAI47_09530 at 2014.125 kb on + strand, within IAI47_09530 at 2014.126 kb on - strand, within IAI47_09530 at 2014.152 kb on + strand, within IAI47_09530 at 2014.266 kb on + strand, within IAI47_09530 at 2014.266 kb on + strand, within IAI47_09530 at 2014.266 kb on + strand, within IAI47_09530 at 2014.350 kb on + strand, within IAI47_09530 at 2014.350 kb on + strand, within IAI47_09530 at 2014.351 kb on - strand, within IAI47_09530 at 2014.410 kb on + strand, within IAI47_09530 at 2014.410 kb on + strand, within IAI47_09530 at 2014.410 kb on + strand, within IAI47_09530 at 2014.411 kb on - strand, within IAI47_09530 at 2014.413 kb on + strand, within IAI47_09530 at 2014.413 kb on + strand, within IAI47_09530 at 2014.413 kb on + strand, within IAI47_09530 at 2014.413 kb on + strand, within IAI47_09530 at 2014.413 kb on + strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.414 kb on - strand, within IAI47_09530 at 2014.479 kb on + strand at 2014.479 kb on + strand at 2014.479 kb on + strand at 2014.479 kb on + strand at 2014.479 kb on + strand at 2014.479 kb on + strand at 2014.480 kb on - strand at 2014.480 kb on - strand at 2014.480 kb on - strand at 2014.480 kb on - strand at 2014.485 kb on + strand at 2014.486 kb on - strand at 2014.554 kb on + strand at 2014.554 kb on + strand at 2014.554 kb on + strand at 2014.555 kb on - strand at 2014.555 kb on - strand at 2014.555 kb on - strand at 2014.582 kb on + strand at 2014.582 kb on + strand at 2014.582 kb on + strand at 2014.583 kb on - strand at 2014.583 kb on - strand at 2014.583 kb on - strand at 2014.583 kb on - strand at 2014.583 kb on - strand at 2014.584 kb on + strand at 2014.584 kb on + strand at 2014.631 kb on - strand at 2014.631 kb on - strand at 2014.641 kb on - strand at 2014.682 kb on + strand at 2014.682 kb on + strand at 2014.682 kb on + strand at 2014.682 kb on + strand at 2014.682 kb on + strand at 2014.683 kb on - strand at 2014.683 kb on - strand at 2014.683 kb on - strand at 2014.686 kb on + strand at 2014.686 kb on + strand at 2014.686 kb on + strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.687 kb on - strand at 2014.689 kb on + strand at 2014.689 kb on + strand at 2014.689 kb on + strand at 2014.689 kb on + strand at 2014.690 kb on - strand at 2014.690 kb on - strand at 2014.694 kb on + strand at 2014.694 kb on + strand at 2014.694 kb on + strand at 2014.694 kb on + strand at 2014.695 kb on - strand at 2014.695 kb on - strand at 2014.695 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 2,011,920 + IAI47_09520 0.77 +0.3 2,011,920 + IAI47_09520 0.77 +0.3 2,011,920 + IAI47_09520 0.77 +1.5 2,011,921 - IAI47_09520 0.77 +1.7 2,012,011 + IAI47_09520 0.79 +1.6 2,012,011 + IAI47_09520 0.79 +2.9 2,012,011 + IAI47_09520 0.79 +2.4 2,012,011 + IAI47_09520 0.79 -0.8 2,012,012 - IAI47_09520 0.79 +1.6 2,012,012 - IAI47_09520 0.79 -0.4 2,012,032 + IAI47_09520 0.80 +0.2 2,012,032 + IAI47_09520 0.80 +0.3 2,012,033 - IAI47_09520 0.80 +0.2 2,012,033 - IAI47_09520 0.80 -0.5 2,012,033 - IAI47_09520 0.80 -0.4 2,012,172 + IAI47_09520 0.83 +0.4 2,012,176 + IAI47_09520 0.83 +0.3 2,012,177 - IAI47_09520 0.83 -0.8 2,012,177 - IAI47_09520 0.83 +1.3 2,012,218 + IAI47_09520 0.84 +1.3 2,012,218 + IAI47_09520 0.84 +2.0 2,012,218 + IAI47_09520 0.84 -1.1 2,012,219 - IAI47_09520 0.84 +0.5 2,012,224 - IAI47_09520 0.84 +0.0 2,012,231 - IAI47_09520 0.85 +0.3 2,012,262 + IAI47_09520 0.85 +0.4 2,012,262 + IAI47_09520 0.85 +1.2 2,012,262 + IAI47_09520 0.85 +0.3 2,012,263 - IAI47_09520 0.85 -0.5 2,012,263 - IAI47_09520 0.85 -0.2 2,012,280 + IAI47_09520 0.86 +0.2 2,012,280 + IAI47_09520 0.86 +2.2 2,012,280 + IAI47_09520 0.86 -0.5 2,012,281 - IAI47_09520 0.86 +0.2 2,012,288 + IAI47_09520 0.86 -0.5 2,012,288 + IAI47_09520 0.86 -1.0 2,012,288 + IAI47_09520 0.86 +0.9 2,012,289 - IAI47_09520 0.86 +0.6 2,012,427 + IAI47_09520 0.90 +0.3 2,012,427 + IAI47_09520 0.90 +0.9 2,012,427 + IAI47_09520 0.90 -0.5 2,012,427 + IAI47_09520 0.90 -0.1 2,012,428 - IAI47_09520 0.90 -0.2 2,012,451 + +3.4 2,012,451 + -1.6 2,012,451 + +0.2 2,012,451 + -1.2 2,012,451 + +1.0 2,012,452 - +0.2 2,012,452 - +0.8 2,012,452 - -1.2 2,012,452 - -0.7 2,012,492 + +0.5 2,012,492 + +2.3 2,012,492 + +0.4 2,012,493 - -0.5 2,012,493 - -0.5 2,012,550 + +2.0 2,012,571 + -0.5 2,012,572 - +0.1 2,012,572 - +0.3 2,012,572 - -0.1 2,012,572 - -0.1 2,012,631 - -0.5 2,012,670 + -0.3 2,012,670 + +0.6 2,012,670 + -0.1 2,012,673 + +0.8 2,012,674 - -0.2 2,012,738 + +0.3 2,012,738 + -0.0 2,012,738 + +1.2 2,012,738 + +0.9 2,012,738 + +1.6 2,012,738 + +0.2 2,012,739 - -1.8 2,012,739 - -1.1 2,012,739 - -0.7 2,012,739 - +0.3 2,012,739 - +0.8 2,012,742 + -0.7 2,012,742 + -0.4 2,012,742 + +1.4 2,012,743 - -0.0 2,012,743 - +1.2 2,012,750 - -0.2 2,012,765 + +0.2 2,012,776 + +0.5 2,012,776 + +1.9 2,012,776 + -0.3 2,012,790 + +1.6 2,012,790 + +1.1 2,012,820 - -1.1 2,012,850 - +0.5 2,012,870 + -0.0 2,012,871 - -0.8 2,012,916 - -1.5 2,012,954 - -0.8 2,012,994 + IAI47_09525 0.13 -1.4 2,013,009 - IAI47_09525 0.15 +0.3 2,013,118 + IAI47_09525 0.29 +0.5 2,013,126 - IAI47_09525 0.30 -0.4 2,013,164 + IAI47_09525 0.34 -1.3 2,013,164 + IAI47_09525 0.34 -0.0 2,013,165 - IAI47_09525 0.34 -1.1 2,013,252 + IAI47_09525 0.45 -0.7 2,013,252 + IAI47_09525 0.45 -1.5 2,013,252 + IAI47_09525 0.45 +0.8 2,013,253 - IAI47_09525 0.45 -0.8 2,013,253 - IAI47_09525 0.45 +0.2 2,013,255 + IAI47_09525 0.46 -1.0 2,013,256 - IAI47_09525 0.46 -0.7 2,013,256 - IAI47_09525 0.46 -0.0 2,013,256 - IAI47_09525 0.46 +3.2 2,013,274 + IAI47_09525 0.48 -1.9 2,013,275 - IAI47_09525 0.48 -0.1 2,013,275 - IAI47_09525 0.48 +1.0 2,013,359 + IAI47_09525 0.58 -0.3 2,013,360 - IAI47_09525 0.59 -0.2 2,013,360 - IAI47_09525 0.59 -0.8 2,013,360 - IAI47_09525 0.59 -0.0 2,013,360 - IAI47_09525 0.59 +0.1 2,013,495 - IAI47_09525 0.75 -0.4 2,013,503 + IAI47_09525 0.76 -1.4 2,013,503 + IAI47_09525 0.76 -0.0 2,013,551 + IAI47_09525 0.82 -0.7 2,013,552 - IAI47_09525 0.82 -0.7 2,013,552 - IAI47_09525 0.82 -0.4 2,013,552 - IAI47_09525 0.82 -0.8 2,013,599 + IAI47_09525 0.88 -0.8 2,013,599 + IAI47_09525 0.88 -0.7 2,013,599 + IAI47_09525 0.88 -1.4 2,013,599 + IAI47_09525 0.88 +0.2 2,013,600 - IAI47_09525 0.88 +0.6 2,013,614 + IAI47_09525 0.90 -2.4 2,013,675 + -0.8 2,013,683 + -0.4 2,013,695 + +0.3 2,013,696 - -0.7 2,013,727 + -1.3 2,013,727 + +0.2 2,013,727 + +0.5 2,013,733 + -1.8 2,013,733 + +0.0 2,013,733 + -0.2 2,013,736 + -0.4 2,013,736 + +0.8 2,013,736 + +0.6 2,013,736 + +1.7 2,013,736 + +0.5 2,013,737 - +1.0 2,013,740 + -1.5 2,013,740 + +0.7 2,013,740 + +0.2 2,013,741 - +1.2 2,013,741 - -0.9 2,013,741 - +1.6 2,013,741 - +1.4 2,013,741 - +0.7 2,013,741 - +1.6 2,013,741 - +0.9 2,013,744 + +0.7 2,013,744 + -1.2 2,013,744 + -0.5 2,013,749 + +0.3 2,013,749 + -0.2 2,013,750 - -0.3 2,013,753 + -0.4 2,013,753 + +0.5 2,013,753 + -1.1 2,013,753 + -1.3 2,013,754 - +0.7 2,013,754 - -1.1 2,013,754 - +1.2 2,013,754 - -1.1 2,013,754 - -1.9 2,013,755 + -0.1 2,013,756 - -1.4 2,013,792 - -0.0 2,013,804 - -0.8 2,013,804 - -0.5 2,013,816 + -1.0 2,013,852 + -0.0 2,013,852 + +2.0 2,013,852 + -2.1 2,013,853 - -0.6 2,013,864 + +0.0 2,013,889 + +0.4 2,013,889 + -0.5 2,013,890 - -1.1 2,013,890 - +0.4 2,013,956 - +1.0 2,013,956 - +1.2 2,013,993 - -0.6 2,014,038 + +1.2 2,014,039 - -0.2 2,014,068 + IAI47_09530 0.15 +0.6 2,014,068 + IAI47_09530 0.15 +1.7 2,014,069 - IAI47_09530 0.15 +0.3 2,014,069 - IAI47_09530 0.15 +0.7 2,014,072 + IAI47_09530 0.15 -0.2 2,014,125 + IAI47_09530 0.25 +2.9 2,014,126 - IAI47_09530 0.25 +0.1 2,014,152 + IAI47_09530 0.30 -0.1 2,014,266 + IAI47_09530 0.52 +1.2 2,014,266 + IAI47_09530 0.52 +0.0 2,014,266 + IAI47_09530 0.52 -0.0 2,014,350 + IAI47_09530 0.68 +0.7 2,014,350 + IAI47_09530 0.68 +1.8 2,014,351 - IAI47_09530 0.68 -0.5 2,014,410 + IAI47_09530 0.79 +0.6 2,014,410 + IAI47_09530 0.79 -0.5 2,014,410 + IAI47_09530 0.79 -0.1 2,014,411 - IAI47_09530 0.79 +0.6 2,014,413 + IAI47_09530 0.79 -0.0 2,014,413 + IAI47_09530 0.79 +2.2 2,014,413 + IAI47_09530 0.79 -1.9 2,014,413 + IAI47_09530 0.79 +1.9 2,014,413 + IAI47_09530 0.79 +3.5 2,014,414 - IAI47_09530 0.80 +0.2 2,014,414 - IAI47_09530 0.80 +0.2 2,014,414 - IAI47_09530 0.80 +1.6 2,014,414 - IAI47_09530 0.80 -0.2 2,014,414 - IAI47_09530 0.80 -0.2 2,014,414 - IAI47_09530 0.80 +0.2 2,014,414 - IAI47_09530 0.80 +0.5 2,014,479 + -0.7 2,014,479 + +1.6 2,014,479 + -1.0 2,014,479 + -0.4 2,014,479 + -0.3 2,014,479 + +0.3 2,014,480 - +1.0 2,014,480 - +0.2 2,014,480 - +1.9 2,014,480 - +0.7 2,014,485 + +2.4 2,014,486 - -0.5 2,014,554 + +0.2 2,014,554 + +1.7 2,014,554 + +2.6 2,014,555 - -0.7 2,014,555 - -0.4 2,014,555 - +0.6 2,014,582 + +1.3 2,014,582 + +0.3 2,014,582 + +0.1 2,014,583 - +1.5 2,014,583 - +0.9 2,014,583 - -0.3 2,014,583 - -1.2 2,014,583 - -1.4 2,014,584 + -0.0 2,014,584 + -0.5 2,014,631 - +0.2 2,014,631 - -0.8 2,014,641 - +0.9 2,014,682 + +2.2 2,014,682 + -0.1 2,014,682 + +1.0 2,014,682 + -1.2 2,014,682 + -0.6 2,014,683 - +0.6 2,014,683 - +0.3 2,014,683 - -0.5 2,014,686 + -0.2 2,014,686 + +0.1 2,014,686 + -0.7 2,014,687 - +1.0 2,014,687 - -0.4 2,014,687 - +0.8 2,014,687 - -1.0 2,014,687 - -0.8 2,014,687 - +0.3 2,014,687 - +0.9 2,014,689 + +1.6 2,014,689 + -0.2 2,014,689 + +0.4 2,014,689 + +0.3 2,014,690 - +1.6 2,014,690 - +0.2 2,014,694 + -0.0 2,014,694 + +0.6 2,014,694 + +0.3 2,014,694 + +2.5 2,014,695 - -1.5 2,014,695 - -0.4 2,014,695 - +0.3
Or see this region's nucleotide sequence