Experiment: a-Ketoglutaric (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0147 and Psyr_0148 are separated by 49 nucleotides Psyr_0148 and Psyr_0149 are separated by 77 nucleotides Psyr_0149 and Psyr_0150 are separated by 114 nucleotides
Psyr_0147: Psyr_0147 - D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding protein, at 159,893 to 160,822
_0147
Psyr_0148: Psyr_0148 - hypothetical protein, at 160,872 to 161,111
_0148
Psyr_0149: Psyr_0149 - succinate semialdehyde dehydrogenase, at 161,189 to 162,646
_0149
Psyr_0150: Psyr_0150 - Acetylornithine deacetylase (ArgE), at 162,761 to 163,918
_0150
Position (kb)
161
162
163 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 160.333 kb on + strand, within Psyr_0147 at 160.333 kb on + strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.334 kb on - strand, within Psyr_0147 at 160.415 kb on + strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.416 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.556 kb on - strand, within Psyr_0147 at 160.817 kb on + strand at 160.820 kb on + strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.821 kb on - strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.857 kb on + strand at 160.858 kb on - strand at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.013 kb on + strand, within Psyr_0148 at 161.014 kb on - strand, within Psyr_0148 at 161.067 kb on + strand, within Psyr_0148 at 161.067 kb on + strand, within Psyr_0148 at 161.128 kb on + strand at 161.190 kb on + strand at 161.190 kb on + strand at 161.191 kb on - strand at 161.227 kb on - strand at 161.227 kb on - strand at 161.388 kb on + strand, within Psyr_0149 at 161.388 kb on + strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.389 kb on - strand, within Psyr_0149 at 161.392 kb on - strand, within Psyr_0149 at 161.392 kb on - strand, within Psyr_0149 at 161.526 kb on - strand, within Psyr_0149 at 161.733 kb on + strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 161.734 kb on - strand, within Psyr_0149 at 162.037 kb on + strand, within Psyr_0149 at 162.258 kb on + strand, within Psyr_0149 at 162.259 kb on - strand, within Psyr_0149 at 162.597 kb on + strand at 162.597 kb on + strand at 162.598 kb on - strand at 162.598 kb on - strand at 162.608 kb on + strand at 162.680 kb on - strand at 162.763 kb on - strand at 162.763 kb on - strand at 162.927 kb on + strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 162.928 kb on - strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.076 kb on + strand, within Psyr_0150 at 163.077 kb on - strand, within Psyr_0150 at 163.217 kb on + strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.218 kb on - strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.227 kb on + strand, within Psyr_0150 at 163.457 kb on + strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.458 kb on - strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.629 kb on + strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150 at 163.630 kb on - strand, within Psyr_0150
Per-strain Table
Position Strand Gene LocusTag Fraction a-Ketoglutaric (C) remove 160,333 + Psyr_0147 0.47 +1.1 160,333 + Psyr_0147 0.47 +0.1 160,334 - Psyr_0147 0.47 +1.0 160,334 - Psyr_0147 0.47 +1.4 160,415 + Psyr_0147 0.56 -1.8 160,416 - Psyr_0147 0.56 +1.8 160,416 - Psyr_0147 0.56 -0.2 160,416 - Psyr_0147 0.56 -1.2 160,556 - Psyr_0147 0.71 -1.0 160,556 - Psyr_0147 0.71 -0.3 160,817 + -1.2 160,820 + +0.1 160,821 - -0.2 160,821 - +2.0 160,821 - -1.9 160,821 - +0.1 160,821 - -0.0 160,857 + +0.2 160,857 + +1.2 160,857 + +0.2 160,857 + -0.8 160,858 - -0.0 161,013 + Psyr_0148 0.59 -0.2 161,013 + Psyr_0148 0.59 +1.1 161,013 + Psyr_0148 0.59 -0.6 161,013 + Psyr_0148 0.59 -1.1 161,013 + Psyr_0148 0.59 -0.9 161,014 - Psyr_0148 0.59 +1.0 161,067 + Psyr_0148 0.81 +0.1 161,067 + Psyr_0148 0.81 -0.1 161,128 + +1.5 161,190 + +0.7 161,190 + -0.0 161,191 - +0.4 161,227 - -0.3 161,227 - +0.2 161,388 + Psyr_0149 0.14 +0.6 161,388 + Psyr_0149 0.14 +0.4 161,389 - Psyr_0149 0.14 +2.1 161,389 - Psyr_0149 0.14 +0.7 161,389 - Psyr_0149 0.14 +1.7 161,389 - Psyr_0149 0.14 +0.2 161,392 - Psyr_0149 0.14 +0.4 161,392 - Psyr_0149 0.14 -0.8 161,526 - Psyr_0149 0.23 +1.4 161,733 + Psyr_0149 0.37 +0.4 161,734 - Psyr_0149 0.37 -2.0 161,734 - Psyr_0149 0.37 +0.7 162,037 + Psyr_0149 0.58 +0.4 162,258 + Psyr_0149 0.73 +2.7 162,259 - Psyr_0149 0.73 -0.2 162,597 + +0.6 162,597 + +0.8 162,598 - +0.8 162,598 - +0.4 162,608 + +0.7 162,680 - +0.4 162,763 - -2.2 162,763 - +0.4 162,927 + Psyr_0150 0.14 +0.6 162,928 - Psyr_0150 0.14 +2.0 162,928 - Psyr_0150 0.14 -0.9 163,076 + Psyr_0150 0.27 +0.1 163,076 + Psyr_0150 0.27 +0.4 163,077 - Psyr_0150 0.27 +0.7 163,217 + Psyr_0150 0.39 +0.6 163,218 - Psyr_0150 0.39 -2.2 163,218 - Psyr_0150 0.39 -0.1 163,218 - Psyr_0150 0.39 +0.2 163,218 - Psyr_0150 0.39 +0.2 163,227 + Psyr_0150 0.40 +1.0 163,227 + Psyr_0150 0.40 -0.1 163,227 + Psyr_0150 0.40 +1.4 163,457 + Psyr_0150 0.60 +1.1 163,458 - Psyr_0150 0.60 +0.0 163,458 - Psyr_0150 0.60 -0.6 163,629 + Psyr_0150 0.75 +0.7 163,629 + Psyr_0150 0.75 -1.9 163,630 - Psyr_0150 0.75 +0.9 163,630 - Psyr_0150 0.75 -0.1 163,630 - Psyr_0150 0.75 -0.1 163,630 - Psyr_0150 0.75 -1.4
Or see this region's nucleotide sequence