Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_824

Experiment: Fusidic 0.4 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_823 and Pf6N2E2_824 overlap by 4 nucleotidesPf6N2E2_824 and Pf6N2E2_825 are separated by 15 nucleotides Pf6N2E2_823 - Outer membrane protein OprN, at 958,560 to 959,981 _823 Pf6N2E2_824 - Multidrug efflux transporter MexF, at 959,978 to 963,157 _824 Pf6N2E2_825 - Multidrug efflux membrane fusion protein MexE, at 963,173 to 964,426 _825 Position (kb) 959 960 961 962 963 964Strain fitness (log2 ratio) -2 -1 0 1 2 3at 959.446 kb on + strand, within Pf6N2E2_823at 959.488 kb on + strand, within Pf6N2E2_823at 959.593 kb on + strand, within Pf6N2E2_823at 959.911 kb on + strandat 959.911 kb on + strandat 960.053 kb on + strandat 960.053 kb on + strandat 960.054 kb on - strandat 960.054 kb on - strandat 960.057 kb on + strandat 960.057 kb on + strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.058 kb on - strandat 960.277 kb on + strandat 960.277 kb on + strandat 960.278 kb on - strandat 960.332 kb on + strand, within Pf6N2E2_824at 960.456 kb on + strand, within Pf6N2E2_824at 960.456 kb on + strand, within Pf6N2E2_824at 960.488 kb on + strand, within Pf6N2E2_824at 960.488 kb on + strand, within Pf6N2E2_824at 960.489 kb on - strand, within Pf6N2E2_824at 960.489 kb on - strand, within Pf6N2E2_824at 960.540 kb on + strand, within Pf6N2E2_824at 960.540 kb on + strand, within Pf6N2E2_824at 960.561 kb on + strand, within Pf6N2E2_824at 960.562 kb on - strand, within Pf6N2E2_824at 960.631 kb on - strand, within Pf6N2E2_824at 960.678 kb on + strand, within Pf6N2E2_824at 960.678 kb on + strand, within Pf6N2E2_824at 960.828 kb on + strand, within Pf6N2E2_824at 960.829 kb on - strand, within Pf6N2E2_824at 960.829 kb on - strand, within Pf6N2E2_824at 960.829 kb on - strand, within Pf6N2E2_824at 960.861 kb on + strand, within Pf6N2E2_824at 960.861 kb on + strand, within Pf6N2E2_824at 960.862 kb on - strand, within Pf6N2E2_824at 960.876 kb on + strand, within Pf6N2E2_824at 960.876 kb on + strand, within Pf6N2E2_824at 960.877 kb on - strand, within Pf6N2E2_824at 960.877 kb on - strand, within Pf6N2E2_824at 960.975 kb on + strand, within Pf6N2E2_824at 960.976 kb on - strand, within Pf6N2E2_824at 960.976 kb on - strand, within Pf6N2E2_824at 961.044 kb on + strand, within Pf6N2E2_824at 961.044 kb on + strand, within Pf6N2E2_824at 961.045 kb on - strandat 961.056 kb on + strand, within Pf6N2E2_824at 961.146 kb on + strand, within Pf6N2E2_824at 961.146 kb on + strand, within Pf6N2E2_824at 961.147 kb on - strand, within Pf6N2E2_824at 961.147 kb on - strand, within Pf6N2E2_824at 961.147 kb on - strand, within Pf6N2E2_824at 961.147 kb on - strand, within Pf6N2E2_824at 961.168 kb on - strand, within Pf6N2E2_824at 961.168 kb on - strand, within Pf6N2E2_824at 961.419 kb on + strand, within Pf6N2E2_824at 961.419 kb on + strand, within Pf6N2E2_824at 961.419 kb on + strand, within Pf6N2E2_824at 961.497 kb on + strand, within Pf6N2E2_824at 961.551 kb on + strand, within Pf6N2E2_824at 961.551 kb on + strand, within Pf6N2E2_824at 961.551 kb on + strand, within Pf6N2E2_824at 961.551 kb on + strand, within Pf6N2E2_824at 961.551 kb on + strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.552 kb on - strand, within Pf6N2E2_824at 961.750 kb on + strand, within Pf6N2E2_824at 961.751 kb on - strand, within Pf6N2E2_824at 961.751 kb on - strand, within Pf6N2E2_824at 961.752 kb on + strand, within Pf6N2E2_824at 961.752 kb on + strand, within Pf6N2E2_824at 961.952 kb on + strand, within Pf6N2E2_824at 961.952 kb on + strand, within Pf6N2E2_824at 961.953 kb on - strand, within Pf6N2E2_824at 961.994 kb on + strand, within Pf6N2E2_824at 961.994 kb on + strand, within Pf6N2E2_824at 961.994 kb on + strand, within Pf6N2E2_824at 961.994 kb on + strand, within Pf6N2E2_824at 961.995 kb on - strand, within Pf6N2E2_824at 961.995 kb on - strand, within Pf6N2E2_824at 961.995 kb on - strand, within Pf6N2E2_824at 961.995 kb on - strand, within Pf6N2E2_824at 962.014 kb on + strand, within Pf6N2E2_824at 962.122 kb on + strand, within Pf6N2E2_824at 962.122 kb on + strand, within Pf6N2E2_824at 962.122 kb on + strand, within Pf6N2E2_824at 962.122 kb on + strand, within Pf6N2E2_824at 962.122 kb on + strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.123 kb on - strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.126 kb on + strand, within Pf6N2E2_824at 962.127 kb on - strand, within Pf6N2E2_824at 962.127 kb on - strand, within Pf6N2E2_824at 962.127 kb on - strand, within Pf6N2E2_824at 962.127 kb on - strand, within Pf6N2E2_824at 962.127 kb on - strand, within Pf6N2E2_824at 962.169 kb on + strand, within Pf6N2E2_824at 962.169 kb on + strand, within Pf6N2E2_824at 962.170 kb on - strand, within Pf6N2E2_824at 962.181 kb on + strand, within Pf6N2E2_824at 962.182 kb on - strand, within Pf6N2E2_824at 962.182 kb on - strand, within Pf6N2E2_824at 962.426 kb on + strand, within Pf6N2E2_824at 962.427 kb on - strand, within Pf6N2E2_824at 962.427 kb on - strand, within Pf6N2E2_824at 962.427 kb on - strand, within Pf6N2E2_824at 962.427 kb on - strand, within Pf6N2E2_824at 962.605 kb on - strand, within Pf6N2E2_824at 962.636 kb on + strand, within Pf6N2E2_824at 962.636 kb on + strand, within Pf6N2E2_824at 962.682 kb on + strand, within Pf6N2E2_824at 962.682 kb on + strand, within Pf6N2E2_824at 962.683 kb on - strand, within Pf6N2E2_824at 962.683 kb on - strand, within Pf6N2E2_824at 962.694 kb on + strand, within Pf6N2E2_824at 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.922 kb on + strandat 962.923 kb on - strandat 962.923 kb on - strandat 962.923 kb on - strandat 962.923 kb on - strandat 962.923 kb on - strandat 962.923 kb on - strandat 963.268 kb on + strandat 963.269 kb on - strandat 963.269 kb on - strandat 963.269 kb on - strandat 963.269 kb on - strandat 963.537 kb on + strand, within Pf6N2E2_825at 963.537 kb on + strand, within Pf6N2E2_825at 963.538 kb on - strand, within Pf6N2E2_825at 963.538 kb on - strand, within Pf6N2E2_825at 963.538 kb on - strand, within Pf6N2E2_825at 963.538 kb on - strand, within Pf6N2E2_825at 963.538 kb on - strand, within Pf6N2E2_825at 963.541 kb on + strand, within Pf6N2E2_825at 963.541 kb on + strand, within Pf6N2E2_825at 963.570 kb on + strand, within Pf6N2E2_825at 963.570 kb on + strand, within Pf6N2E2_825at 963.570 kb on + strand, within Pf6N2E2_825at 963.570 kb on + strand, within Pf6N2E2_825at 963.571 kb on - strand, within Pf6N2E2_825at 963.585 kb on + strand, within Pf6N2E2_825at 963.586 kb on - strand, within Pf6N2E2_825at 963.711 kb on + strand, within Pf6N2E2_825at 963.711 kb on + strand, within Pf6N2E2_825at 963.712 kb on - strand, within Pf6N2E2_825at 963.759 kb on + strand, within Pf6N2E2_825at 963.759 kb on + strand, within Pf6N2E2_825at 963.760 kb on - strand, within Pf6N2E2_825at 963.792 kb on + strand, within Pf6N2E2_825at 963.798 kb on + strand, within Pf6N2E2_825at 963.799 kb on - strand, within Pf6N2E2_825at 964.118 kb on + strand, within Pf6N2E2_825at 964.119 kb on - strand, within Pf6N2E2_825

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml
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959,446 + Pf6N2E2_823 0.62 -0.3
959,488 + Pf6N2E2_823 0.65 +0.5
959,593 + Pf6N2E2_823 0.73 -0.6
959,911 + -0.9
959,911 + +0.4
960,053 + -2.3
960,053 + -0.2
960,054 - +0.5
960,054 - +0.4
960,057 + -2.3
960,057 + +1.3
960,058 - -0.3
960,058 - +1.4
960,058 - +0.2
960,058 - +1.4
960,058 - +1.5
960,058 - +2.1
960,058 - -1.0
960,058 - +0.4
960,277 + +0.4
960,277 + +1.7
960,278 - -0.5
960,332 + Pf6N2E2_824 0.11 -0.2
960,456 + Pf6N2E2_824 0.15 +0.2
960,456 + Pf6N2E2_824 0.15 +1.9
960,488 + Pf6N2E2_824 0.16 +0.5
960,488 + Pf6N2E2_824 0.16 +1.2
960,489 - Pf6N2E2_824 0.16 -0.7
960,489 - Pf6N2E2_824 0.16 +0.5
960,540 + Pf6N2E2_824 0.18 +0.6
960,540 + Pf6N2E2_824 0.18 +1.1
960,561 + Pf6N2E2_824 0.18 +1.1
960,562 - Pf6N2E2_824 0.18 -1.7
960,631 - Pf6N2E2_824 0.21 -0.5
960,678 + Pf6N2E2_824 0.22 +0.5
960,678 + Pf6N2E2_824 0.22 +1.0
960,828 + Pf6N2E2_824 0.27 -0.6
960,829 - Pf6N2E2_824 0.27 +0.5
960,829 - Pf6N2E2_824 0.27 +0.2
960,829 - Pf6N2E2_824 0.27 +1.1
960,861 + Pf6N2E2_824 0.28 +1.0
960,861 + Pf6N2E2_824 0.28 +0.2
960,862 - Pf6N2E2_824 0.28 +0.6
960,876 + Pf6N2E2_824 0.28 +1.6
960,876 + Pf6N2E2_824 0.28 -1.3
960,877 - Pf6N2E2_824 0.28 +0.5
960,877 - Pf6N2E2_824 0.28 +2.7
960,975 + Pf6N2E2_824 0.31 -0.0
960,976 - Pf6N2E2_824 0.31 +0.5
960,976 - Pf6N2E2_824 0.31 +1.6
961,044 + Pf6N2E2_824 0.34 +1.0
961,044 + Pf6N2E2_824 0.34 +1.5
961,045 - -0.5
961,056 + Pf6N2E2_824 0.34 +1.4
961,146 + Pf6N2E2_824 0.37 +1.0
961,146 + Pf6N2E2_824 0.37 +0.4
961,147 - Pf6N2E2_824 0.37 +1.2
961,147 - Pf6N2E2_824 0.37 -2.5
961,147 - Pf6N2E2_824 0.37 +1.5
961,147 - Pf6N2E2_824 0.37 +1.3
961,168 - Pf6N2E2_824 0.37 -0.1
961,168 - Pf6N2E2_824 0.37 +1.6
961,419 + Pf6N2E2_824 0.45 -0.1
961,419 + Pf6N2E2_824 0.45 +0.3
961,419 + Pf6N2E2_824 0.45 -1.3
961,497 + Pf6N2E2_824 0.48 -2.0
961,551 + Pf6N2E2_824 0.49 -0.8
961,551 + Pf6N2E2_824 0.49 +1.0
961,551 + Pf6N2E2_824 0.49 -1.9
961,551 + Pf6N2E2_824 0.49 +1.0
961,551 + Pf6N2E2_824 0.49 +1.3
961,552 - Pf6N2E2_824 0.49 -0.0
961,552 - Pf6N2E2_824 0.49 +1.3
961,552 - Pf6N2E2_824 0.49 +2.5
961,552 - Pf6N2E2_824 0.49 +1.0
961,552 - Pf6N2E2_824 0.49 +1.5
961,552 - Pf6N2E2_824 0.49 +0.7
961,750 + Pf6N2E2_824 0.56 +0.5
961,751 - Pf6N2E2_824 0.56 +0.2
961,751 - Pf6N2E2_824 0.56 +1.5
961,752 + Pf6N2E2_824 0.56 +0.0
961,752 + Pf6N2E2_824 0.56 -1.4
961,952 + Pf6N2E2_824 0.62 +0.9
961,952 + Pf6N2E2_824 0.62 +0.6
961,953 - Pf6N2E2_824 0.62 +0.3
961,994 + Pf6N2E2_824 0.63 +0.4
961,994 + Pf6N2E2_824 0.63 -0.5
961,994 + Pf6N2E2_824 0.63 +1.1
961,994 + Pf6N2E2_824 0.63 +0.9
961,995 - Pf6N2E2_824 0.63 +0.1
961,995 - Pf6N2E2_824 0.63 -0.4
961,995 - Pf6N2E2_824 0.63 +0.6
961,995 - Pf6N2E2_824 0.63 -0.5
962,014 + Pf6N2E2_824 0.64 -0.5
962,122 + Pf6N2E2_824 0.67 +1.6
962,122 + Pf6N2E2_824 0.67 +2.1
962,122 + Pf6N2E2_824 0.67 +1.1
962,122 + Pf6N2E2_824 0.67 +1.7
962,122 + Pf6N2E2_824 0.67 +0.9
962,123 - Pf6N2E2_824 0.67 +0.7
962,123 - Pf6N2E2_824 0.67 -1.9
962,123 - Pf6N2E2_824 0.67 -0.3
962,123 - Pf6N2E2_824 0.67 +1.8
962,123 - Pf6N2E2_824 0.67 +0.9
962,123 - Pf6N2E2_824 0.67 +0.7
962,123 - Pf6N2E2_824 0.67 +0.8
962,123 - Pf6N2E2_824 0.67 +0.3
962,126 + Pf6N2E2_824 0.68 +0.5
962,126 + Pf6N2E2_824 0.68 +2.3
962,126 + Pf6N2E2_824 0.68 +1.0
962,126 + Pf6N2E2_824 0.68 +0.1
962,126 + Pf6N2E2_824 0.68 +0.5
962,126 + Pf6N2E2_824 0.68 +1.1
962,126 + Pf6N2E2_824 0.68 +0.8
962,127 - Pf6N2E2_824 0.68 +0.3
962,127 - Pf6N2E2_824 0.68 +2.1
962,127 - Pf6N2E2_824 0.68 +2.9
962,127 - Pf6N2E2_824 0.68 +0.6
962,127 - Pf6N2E2_824 0.68 +0.8
962,169 + Pf6N2E2_824 0.69 -0.1
962,169 + Pf6N2E2_824 0.69 +0.8
962,170 - Pf6N2E2_824 0.69 +1.3
962,181 + Pf6N2E2_824 0.69 +0.2
962,182 - Pf6N2E2_824 0.69 +1.1
962,182 - Pf6N2E2_824 0.69 +0.4
962,426 + Pf6N2E2_824 0.77 +2.4
962,427 - Pf6N2E2_824 0.77 -0.0
962,427 - Pf6N2E2_824 0.77 +2.4
962,427 - Pf6N2E2_824 0.77 -0.5
962,427 - Pf6N2E2_824 0.77 +1.7
962,605 - Pf6N2E2_824 0.83 +0.7
962,636 + Pf6N2E2_824 0.84 +0.8
962,636 + Pf6N2E2_824 0.84 -0.5
962,682 + Pf6N2E2_824 0.85 +1.0
962,682 + Pf6N2E2_824 0.85 -0.0
962,683 - Pf6N2E2_824 0.85 +2.5
962,683 - Pf6N2E2_824 0.85 +1.1
962,694 + Pf6N2E2_824 0.85 -0.2
962,922 + +1.0
962,922 + -0.0
962,922 + +1.3
962,922 + +2.9
962,922 + -0.8
962,922 + +0.3
962,922 + +1.4
962,922 + +1.0
962,922 + +1.6
962,922 + -0.5
962,923 - +0.2
962,923 - +0.5
962,923 - +2.3
962,923 - +0.5
962,923 - +0.6
962,923 - +0.8
963,268 + -0.4
963,269 - -0.4
963,269 - +0.4
963,269 - -0.6
963,269 - +0.9
963,537 + Pf6N2E2_825 0.29 -0.1
963,537 + Pf6N2E2_825 0.29 +0.3
963,538 - Pf6N2E2_825 0.29 -0.9
963,538 - Pf6N2E2_825 0.29 +1.5
963,538 - Pf6N2E2_825 0.29 +0.8
963,538 - Pf6N2E2_825 0.29 +0.4
963,538 - Pf6N2E2_825 0.29 +0.7
963,541 + Pf6N2E2_825 0.29 +1.8
963,541 + Pf6N2E2_825 0.29 +2.5
963,570 + Pf6N2E2_825 0.32 +0.3
963,570 + Pf6N2E2_825 0.32 -0.5
963,570 + Pf6N2E2_825 0.32 +1.4
963,570 + Pf6N2E2_825 0.32 +0.3
963,571 - Pf6N2E2_825 0.32 +0.9
963,585 + Pf6N2E2_825 0.33 +0.8
963,586 - Pf6N2E2_825 0.33 -0.3
963,711 + Pf6N2E2_825 0.43 +0.5
963,711 + Pf6N2E2_825 0.43 +0.0
963,712 - Pf6N2E2_825 0.43 -0.1
963,759 + Pf6N2E2_825 0.47 -2.6
963,759 + Pf6N2E2_825 0.47 +0.9
963,760 - Pf6N2E2_825 0.47 +0.0
963,792 + Pf6N2E2_825 0.49 +1.9
963,798 + Pf6N2E2_825 0.50 +1.0
963,799 - Pf6N2E2_825 0.50 +2.0
964,118 + Pf6N2E2_825 0.75 +1.2
964,119 - Pf6N2E2_825 0.75 -0.4

Or see this region's nucleotide sequence