Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_664 and Pf6N2E2_665 are separated by 2 nucleotides Pf6N2E2_665 and Pf6N2E2_666 are separated by 36 nucleotides
Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025
_664
Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005
_665
Pf6N2E2_666 - Acetoin dehydrogenase E1 component beta-subunit (EC 1.2.4.-), at 771,042 to 772,058
_666
Position (kb) 770
771
772 Strain fitness (log2 ratio) -2
-1
0
1
2 at 769.432 kb on + strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664 at 769.433 kb on - strand, within Pf6N2E2_664 at 769.939 kb on + strand at 770.155 kb on - strand, within Pf6N2E2_665 at 770.155 kb on - strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.289 kb on + strand, within Pf6N2E2_665 at 770.290 kb on - strand, within Pf6N2E2_665 at 770.560 kb on - strand, within Pf6N2E2_665 at 770.934 kb on + strand at 770.988 kb on + strand at 770.989 kb on - strand at 770.998 kb on - strand at 771.028 kb on + strand at 771.028 kb on + strand at 771.029 kb on - strand at 771.029 kb on - strand at 771.378 kb on + strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.379 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.506 kb on - strand, within Pf6N2E2_666 at 771.526 kb on - strand, within Pf6N2E2_666 at 771.601 kb on - strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.633 kb on + strand, within Pf6N2E2_666 at 771.776 kb on - strand, within Pf6N2E2_666 at 771.884 kb on + strand, within Pf6N2E2_666 at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.993 kb on + strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand at 771.994 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 769,432 + Pf6N2E2_664 0.45 +0.9 769,433 - Pf6N2E2_664 0.45 +1.0 769,433 - Pf6N2E2_664 0.45 +1.3 769,939 + +1.0 770,155 - Pf6N2E2_665 0.13 -0.0 770,155 - Pf6N2E2_665 0.13 +1.0 770,289 + Pf6N2E2_665 0.27 +1.0 770,289 + Pf6N2E2_665 0.27 +1.0 770,290 - Pf6N2E2_665 0.27 +0.8 770,560 - Pf6N2E2_665 0.54 +1.2 770,934 + +0.8 770,988 + -0.5 770,989 - -0.4 770,998 - +0.2 771,028 + -0.5 771,028 + +0.3 771,029 - -1.1 771,029 - -2.5 771,378 + Pf6N2E2_666 0.33 +0.1 771,379 - Pf6N2E2_666 0.33 -0.7 771,379 - Pf6N2E2_666 0.33 +0.6 771,506 - Pf6N2E2_666 0.46 -0.3 771,506 - Pf6N2E2_666 0.46 +0.3 771,526 - Pf6N2E2_666 0.48 -1.6 771,601 - Pf6N2E2_666 0.55 +0.2 771,633 + Pf6N2E2_666 0.58 -0.4 771,633 + Pf6N2E2_666 0.58 -0.5 771,776 - Pf6N2E2_666 0.72 -2.1 771,884 + Pf6N2E2_666 0.83 +1.6 771,993 + -0.7 771,993 + -0.7 771,993 + -2.3 771,993 + -1.0 771,993 + -0.3 771,994 - +2.2 771,994 - +0.2 771,994 - +0.4 771,994 - +1.1 771,994 - +0.8
Or see this region's nucleotide sequence