Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_58 and Pf6N2E2_59 are separated by 120 nucleotides Pf6N2E2_59 and Pf6N2E2_60 are separated by 193 nucleotides
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Pf6N2E2_59 - 16 kDa heat shock protein A, at 97,966 to 98,418
_59
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Position (kb) 97
98
99 Strain fitness (log2 ratio) -1
0
1
2 at 97.377 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.411 kb on + strand, within Pf6N2E2_58 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.894 kb on - strand at 97.894 kb on - strand at 97.971 kb on + strand at 98.072 kb on - strand, within Pf6N2E2_59 at 98.072 kb on - strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.081 kb on - strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.228 kb on - strand, within Pf6N2E2_59 at 98.462 kb on + strand at 98.468 kb on + strand at 98.468 kb on + strand at 98.469 kb on - strand at 98.469 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.546 kb on + strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.614 kb on - strand at 98.664 kb on + strand at 98.664 kb on + strand at 98.665 kb on - strand at 98.673 kb on + strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 99.034 kb on + strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 97,377 + Pf6N2E2_58 0.51 -1.2 97,411 + Pf6N2E2_58 0.55 -0.5 97,411 + Pf6N2E2_58 0.55 -0.9 97,889 + +0.1 97,889 + +0.2 97,889 + +2.3 97,889 + +0.2 97,889 + +0.4 97,889 + +0.4 97,889 + -0.7 97,894 - -1.4 97,894 - -0.1 97,971 + -0.5 98,072 - Pf6N2E2_59 0.23 -1.2 98,072 - Pf6N2E2_59 0.23 +0.4 98,080 + Pf6N2E2_59 0.25 -0.3 98,080 + Pf6N2E2_59 0.25 -0.7 98,080 + Pf6N2E2_59 0.25 -0.6 98,080 + Pf6N2E2_59 0.25 +1.5 98,081 - Pf6N2E2_59 0.25 +2.3 98,227 + Pf6N2E2_59 0.58 -0.2 98,227 + Pf6N2E2_59 0.58 +0.9 98,228 - Pf6N2E2_59 0.58 +0.7 98,462 + -0.0 98,468 + -0.1 98,468 + -0.7 98,469 - +0.3 98,469 - +0.2 98,531 - -0.7 98,531 - +1.0 98,531 - +1.0 98,531 - -0.7 98,546 + -0.0 98,547 - +1.5 98,547 - +0.3 98,547 - -0.6 98,614 - +0.6 98,664 + -0.5 98,664 + -0.2 98,665 - -0.7 98,673 + +0.2 98,674 - +0.1 98,674 - -0.1 98,674 - +0.0 98,675 + +0.5 98,675 + -0.2 98,675 + -0.9 98,675 + -1.5 98,675 + -1.1 98,675 + -0.3 98,675 + +1.3 98,675 + +0.6 98,675 + +1.2 98,675 + +0.3 98,675 + -0.3 98,676 - +0.3 98,676 - +0.2 98,676 - -0.2 98,676 - -0.5 98,676 - -0.7 98,676 - +0.4 98,676 - +2.2 98,676 - -0.4 98,676 - +0.3 98,676 - -0.1 98,676 - +1.3 98,676 - -0.8 98,676 - -0.4 98,676 - +0.7 99,034 + Pf6N2E2_60 0.13 +1.3 99,035 - Pf6N2E2_60 0.13 -0.0 99,035 - Pf6N2E2_60 0.13 -0.5 99,035 - Pf6N2E2_60 0.13 -0.6
Or see this region's nucleotide sequence