Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5534 and Pf6N2E2_5535 are separated by 181 nucleotides Pf6N2E2_5535 and Pf6N2E2_5536 are separated by 113 nucleotides Pf6N2E2_5536 and Pf6N2E2_5537 are separated by 0 nucleotides
Pf6N2E2_5534 - FIG000859: hypothetical protein YebC, at 6,350,944 to 6,351,690
_5534
Pf6N2E2_5535 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 6,351,872 to 6,352,396
_5535
Pf6N2E2_5536 - Holliday junction DNA helicase RuvA, at 6,352,510 to 6,353,118
_5536
Pf6N2E2_5537 - Holliday junction DNA helicase RuvB, at 6,353,119 to 6,354,180
_5537
Position (kb)
6351
6352
6353 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 6350.876 kb on + strand at 6350.876 kb on + strand at 6350.877 kb on - strand at 6350.901 kb on - strand at 6351.202 kb on + strand, within Pf6N2E2_5534 at 6351.202 kb on + strand, within Pf6N2E2_5534 at 6351.202 kb on + strand, within Pf6N2E2_5534 at 6351.202 kb on + strand, within Pf6N2E2_5534 at 6351.203 kb on - strand, within Pf6N2E2_5534 at 6351.203 kb on - strand, within Pf6N2E2_5534 at 6351.268 kb on - strand, within Pf6N2E2_5534 at 6351.484 kb on + strand, within Pf6N2E2_5534 at 6351.484 kb on + strand, within Pf6N2E2_5534 at 6351.484 kb on + strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.485 kb on - strand, within Pf6N2E2_5534 at 6351.847 kb on - strand at 6351.855 kb on + strand at 6351.872 kb on - strand at 6351.879 kb on + strand at 6352.572 kb on - strand, within Pf6N2E2_5536 at 6353.215 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 6,350,876 + +0.9 6,350,876 + -1.7 6,350,877 - -0.7 6,350,901 - -0.8 6,351,202 + Pf6N2E2_5534 0.35 -0.3 6,351,202 + Pf6N2E2_5534 0.35 -1.1 6,351,202 + Pf6N2E2_5534 0.35 +0.9 6,351,202 + Pf6N2E2_5534 0.35 +0.5 6,351,203 - Pf6N2E2_5534 0.35 -0.5 6,351,203 - Pf6N2E2_5534 0.35 -0.2 6,351,268 - Pf6N2E2_5534 0.43 -1.4 6,351,484 + Pf6N2E2_5534 0.72 +1.1 6,351,484 + Pf6N2E2_5534 0.72 -0.3 6,351,484 + Pf6N2E2_5534 0.72 +0.6 6,351,485 - Pf6N2E2_5534 0.72 +0.9 6,351,485 - Pf6N2E2_5534 0.72 -2.8 6,351,485 - Pf6N2E2_5534 0.72 +0.2 6,351,485 - Pf6N2E2_5534 0.72 +0.8 6,351,485 - Pf6N2E2_5534 0.72 +0.1 6,351,485 - Pf6N2E2_5534 0.72 -2.5 6,351,847 - -1.4 6,351,855 + +0.9 6,351,872 - -0.4 6,351,879 + -1.2 6,352,572 - Pf6N2E2_5536 0.10 -0.2 6,353,215 + -2.0
Or see this region's nucleotide sequence