Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_517

Experiment: Fusidic 0.4 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_516 and Pf6N2E2_517 are separated by 148 nucleotidesPf6N2E2_517 and Pf6N2E2_518 are separated by 31 nucleotides Pf6N2E2_516 - Inosose isomerase (EC 5.3.99.-), at 585,558 to 586,379 _516 Pf6N2E2_517 - Epi-inositol hydrolase (EC 3.7.1.-) (from data), at 586,528 to 588,459 _517 Pf6N2E2_518 - Myo-inositol 2-dehydrogenase (EC 1.1.1.18), at 588,491 to 589,501 _518 Position (kb) 586 587 588 589Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 585.553 kb on + strandat 585.553 kb on + strandat 585.553 kb on + strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.554 kb on - strandat 585.727 kb on + strand, within Pf6N2E2_516at 585.728 kb on - strand, within Pf6N2E2_516at 585.829 kb on - strand, within Pf6N2E2_516at 585.969 kb on + strand, within Pf6N2E2_516at 585.970 kb on - strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.063 kb on + strand, within Pf6N2E2_516at 586.064 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.279 kb on - strand, within Pf6N2E2_516at 586.441 kb on - strandat 586.441 kb on - strandat 586.585 kb on + strandat 586.585 kb on + strandat 586.586 kb on - strandat 586.780 kb on + strand, within Pf6N2E2_517at 586.781 kb on - strand, within Pf6N2E2_517at 586.781 kb on - strand, within Pf6N2E2_517at 586.868 kb on + strand, within Pf6N2E2_517at 586.869 kb on - strandat 586.869 kb on - strand, within Pf6N2E2_517at 586.869 kb on - strand, within Pf6N2E2_517at 586.885 kb on + strand, within Pf6N2E2_517at 586.982 kb on - strand, within Pf6N2E2_517at 587.111 kb on - strand, within Pf6N2E2_517at 587.126 kb on - strand, within Pf6N2E2_517at 587.183 kb on + strand, within Pf6N2E2_517at 587.183 kb on + strand, within Pf6N2E2_517at 587.512 kb on - strand, within Pf6N2E2_517at 587.675 kb on - strand, within Pf6N2E2_517at 587.686 kb on + strand, within Pf6N2E2_517at 587.847 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.895 kb on + strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.896 kb on - strand, within Pf6N2E2_517at 587.899 kb on + strandat 587.899 kb on + strand, within Pf6N2E2_517at 587.899 kb on + strand, within Pf6N2E2_517at 587.900 kb on - strand, within Pf6N2E2_517at 587.911 kb on + strand, within Pf6N2E2_517at 587.912 kb on - strand, within Pf6N2E2_517at 587.933 kb on - strand, within Pf6N2E2_517at 587.986 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.010 kb on + strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.011 kb on - strand, within Pf6N2E2_517at 588.227 kb on - strand, within Pf6N2E2_517at 588.241 kb on + strand, within Pf6N2E2_517at 588.242 kb on - strand, within Pf6N2E2_517at 588.242 kb on - strand, within Pf6N2E2_517at 588.352 kb on + strandat 588.352 kb on + strandat 588.352 kb on + strandat 588.353 kb on - strandat 588.353 kb on - strandat 588.454 kb on + strandat 588.472 kb on + strandat 588.473 kb on - strandat 588.473 kb on - strandat 588.473 kb on - strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.488 kb on + strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.489 kb on - strandat 588.541 kb on + strandat 588.653 kb on + strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.876 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 588.981 kb on - strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.055 kb on + strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.056 kb on - strand, within Pf6N2E2_518at 589.188 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518at 589.329 kb on - strand, within Pf6N2E2_518

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Per-strain Table

Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml
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585,553 + +0.6
585,553 + +1.5
585,553 + +0.8
585,554 - +0.8
585,554 - -2.9
585,554 - -0.1
585,554 - +1.0
585,554 - +2.3
585,554 - -0.7
585,554 - +0.4
585,554 - -0.1
585,727 + Pf6N2E2_516 0.21 -1.5
585,728 - Pf6N2E2_516 0.21 -0.3
585,829 - Pf6N2E2_516 0.33 +0.1
585,969 + Pf6N2E2_516 0.50 -1.3
585,970 - Pf6N2E2_516 0.50 +0.0
586,063 + Pf6N2E2_516 0.61 +0.4
586,063 + Pf6N2E2_516 0.61 +1.3
586,063 + Pf6N2E2_516 0.61 -1.2
586,064 - Pf6N2E2_516 0.62 +1.0
586,279 - Pf6N2E2_516 0.88 +0.3
586,279 - Pf6N2E2_516 0.88 -1.1
586,279 - Pf6N2E2_516 0.88 -0.5
586,441 - +1.5
586,441 - +1.3
586,585 + -1.0
586,585 + +1.0
586,586 - +0.4
586,780 + Pf6N2E2_517 0.13 -0.9
586,781 - Pf6N2E2_517 0.13 +0.1
586,781 - Pf6N2E2_517 0.13 -0.3
586,868 + Pf6N2E2_517 0.18 +0.7
586,869 - -1.1
586,869 - Pf6N2E2_517 0.18 -0.1
586,869 - Pf6N2E2_517 0.18 -0.4
586,885 + Pf6N2E2_517 0.18 +0.4
586,982 - Pf6N2E2_517 0.23 +0.9
587,111 - Pf6N2E2_517 0.30 -0.0
587,126 - Pf6N2E2_517 0.31 +1.6
587,183 + Pf6N2E2_517 0.34 -0.3
587,183 + Pf6N2E2_517 0.34 +0.8
587,512 - Pf6N2E2_517 0.51 -0.6
587,675 - Pf6N2E2_517 0.59 +0.8
587,686 + Pf6N2E2_517 0.60 +1.1
587,847 + Pf6N2E2_517 0.68 +0.6
587,895 + Pf6N2E2_517 0.71 -0.0
587,895 + Pf6N2E2_517 0.71 -0.2
587,895 + Pf6N2E2_517 0.71 +0.3
587,895 + Pf6N2E2_517 0.71 +0.8
587,895 + Pf6N2E2_517 0.71 +0.8
587,896 - Pf6N2E2_517 0.71 +3.0
587,896 - Pf6N2E2_517 0.71 -0.1
587,896 - Pf6N2E2_517 0.71 -1.3
587,896 - Pf6N2E2_517 0.71 -1.0
587,896 - Pf6N2E2_517 0.71 +1.3
587,896 - Pf6N2E2_517 0.71 +0.1
587,896 - Pf6N2E2_517 0.71 -0.7
587,899 + +1.3
587,899 + Pf6N2E2_517 0.71 +1.8
587,899 + Pf6N2E2_517 0.71 +4.3
587,900 - Pf6N2E2_517 0.71 +0.3
587,911 + Pf6N2E2_517 0.72 -0.1
587,912 - Pf6N2E2_517 0.72 -0.1
587,933 - Pf6N2E2_517 0.73 -0.4
587,986 + Pf6N2E2_517 0.75 +1.8
588,010 + Pf6N2E2_517 0.77 -0.2
588,010 + Pf6N2E2_517 0.77 +1.8
588,010 + Pf6N2E2_517 0.77 +1.4
588,010 + Pf6N2E2_517 0.77 +1.0
588,010 + Pf6N2E2_517 0.77 +1.4
588,011 - Pf6N2E2_517 0.77 +0.1
588,011 - Pf6N2E2_517 0.77 +0.8
588,011 - Pf6N2E2_517 0.77 +0.4
588,011 - Pf6N2E2_517 0.77 -0.3
588,011 - Pf6N2E2_517 0.77 +1.3
588,227 - Pf6N2E2_517 0.88 -1.0
588,241 + Pf6N2E2_517 0.89 +0.5
588,242 - Pf6N2E2_517 0.89 +1.3
588,242 - Pf6N2E2_517 0.89 +0.7
588,352 + -0.7
588,352 + +0.6
588,352 + +1.0
588,353 - -0.3
588,353 - +1.4
588,454 + +1.3
588,472 + -1.6
588,473 - -1.6
588,473 - +1.1
588,473 - -1.9
588,488 + -0.8
588,488 + +0.3
588,488 + -0.4
588,488 + +0.4
588,488 + +1.6
588,488 + +0.6
588,488 + +0.3
588,489 - +0.7
588,489 - -0.7
588,489 - +0.1
588,489 - +0.1
588,489 - -1.2
588,489 - -1.6
588,489 - +0.4
588,489 - -0.1
588,489 - +1.6
588,541 + +1.0
588,653 + Pf6N2E2_518 0.16 +0.1
588,876 - Pf6N2E2_518 0.38 +0.2
588,876 - Pf6N2E2_518 0.38 +0.4
588,876 - Pf6N2E2_518 0.38 +0.1
588,981 - Pf6N2E2_518 0.48 -0.9
588,981 - Pf6N2E2_518 0.48 +1.0
588,981 - Pf6N2E2_518 0.48 -1.2
589,055 + Pf6N2E2_518 0.56 +1.8
589,055 + Pf6N2E2_518 0.56 +0.6
589,055 + Pf6N2E2_518 0.56 -1.2
589,055 + Pf6N2E2_518 0.56 +1.1
589,055 + Pf6N2E2_518 0.56 +0.4
589,055 + Pf6N2E2_518 0.56 +0.3
589,055 + Pf6N2E2_518 0.56 -0.9
589,055 + Pf6N2E2_518 0.56 -0.8
589,056 - Pf6N2E2_518 0.56 +0.3
589,056 - Pf6N2E2_518 0.56 +1.3
589,056 - Pf6N2E2_518 0.56 -1.1
589,188 - Pf6N2E2_518 0.69 -0.1
589,329 - Pf6N2E2_518 0.83 +0.7
589,329 - Pf6N2E2_518 0.83 +0.1

Or see this region's nucleotide sequence