Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3564 and Pf6N2E2_3565 are separated by 57 nucleotides Pf6N2E2_3565 and Pf6N2E2_3566 are separated by 112 nucleotides Pf6N2E2_3566 and Pf6N2E2_3567 are separated by 376 nucleotides Pf6N2E2_3567 and Pf6N2E2_3568 are separated by 158 nucleotides
Pf6N2E2_3564 - Glycine cleavage system transcriptional activator, at 4,155,243 to 4,156,184
_3564
Pf6N2E2_3565 - Bacterioferritin, at 4,156,242 to 4,156,772
_3565
Pf6N2E2_3566 - exported protein, conserved, at 4,156,885 to 4,158,960
_3566
Pf6N2E2_3567 - FIG00963947: hypothetical protein, at 4,159,337 to 4,159,753
_3567
Pf6N2E2_3568 - Unsaturated fatty acid biosythesis repressor FabR, TetR family, at 4,159,912 to 4,160,544
_3568
Position (kb)
4156
4157
4158
4159 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4155.901 kb on - strand, within Pf6N2E2_3564 at 4155.916 kb on - strand, within Pf6N2E2_3564 at 4155.916 kb on - strand, within Pf6N2E2_3564 at 4155.965 kb on + strand, within Pf6N2E2_3564 at 4155.965 kb on + strand, within Pf6N2E2_3564 at 4156.042 kb on - strand, within Pf6N2E2_3564 at 4156.182 kb on + strand at 4156.183 kb on - strand at 4156.183 kb on - strand at 4156.258 kb on + strand at 4156.258 kb on + strand at 4156.267 kb on + strand at 4156.268 kb on - strand at 4156.268 kb on - strand at 4156.268 kb on - strand at 4156.268 kb on - strand at 4156.268 kb on - strand at 4156.327 kb on - strand, within Pf6N2E2_3565 at 4156.402 kb on - strand, within Pf6N2E2_3565 at 4156.413 kb on + strand, within Pf6N2E2_3565 at 4156.413 kb on + strand, within Pf6N2E2_3565 at 4156.414 kb on - strand, within Pf6N2E2_3565 at 4156.414 kb on - strand, within Pf6N2E2_3565 at 4156.414 kb on - strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.578 kb on + strand, within Pf6N2E2_3565 at 4156.579 kb on - strand, within Pf6N2E2_3565 at 4156.611 kb on + strand, within Pf6N2E2_3565 at 4156.611 kb on + strand, within Pf6N2E2_3565 at 4156.611 kb on + strand, within Pf6N2E2_3565 at 4156.612 kb on - strand, within Pf6N2E2_3565 at 4156.612 kb on - strand, within Pf6N2E2_3565 at 4156.612 kb on - strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.668 kb on + strand, within Pf6N2E2_3565 at 4156.669 kb on - strand, within Pf6N2E2_3565 at 4156.773 kb on + strand at 4156.774 kb on - strand at 4156.779 kb on + strand at 4156.779 kb on + strand at 4156.779 kb on + strand at 4156.780 kb on - strand at 4156.785 kb on + strand at 4157.102 kb on + strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.103 kb on - strand, within Pf6N2E2_3566 at 4157.201 kb on + strand, within Pf6N2E2_3566 at 4157.201 kb on + strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.202 kb on - strand, within Pf6N2E2_3566 at 4157.327 kb on + strand, within Pf6N2E2_3566 at 4157.327 kb on + strand, within Pf6N2E2_3566 at 4157.327 kb on + strand, within Pf6N2E2_3566 at 4157.327 kb on + strand, within Pf6N2E2_3566 at 4157.327 kb on + strand, within Pf6N2E2_3566 at 4157.328 kb on - strand, within Pf6N2E2_3566 at 4157.328 kb on - strand, within Pf6N2E2_3566 at 4157.328 kb on - strand, within Pf6N2E2_3566 at 4157.328 kb on - strand, within Pf6N2E2_3566 at 4157.670 kb on + strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.671 kb on - strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.672 kb on + strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.673 kb on - strand, within Pf6N2E2_3566 at 4157.700 kb on + strand, within Pf6N2E2_3566 at 4157.930 kb on + strand, within Pf6N2E2_3566 at 4157.930 kb on + strand, within Pf6N2E2_3566 at 4157.930 kb on + strand, within Pf6N2E2_3566 at 4157.930 kb on + strand, within Pf6N2E2_3566 at 4157.930 kb on + strand, within Pf6N2E2_3566 at 4157.931 kb on - strand, within Pf6N2E2_3566 at 4157.931 kb on - strand, within Pf6N2E2_3566 at 4157.931 kb on - strand, within Pf6N2E2_3566 at 4157.949 kb on + strand, within Pf6N2E2_3566 at 4158.284 kb on + strand, within Pf6N2E2_3566 at 4158.284 kb on + strand, within Pf6N2E2_3566 at 4158.284 kb on + strand, within Pf6N2E2_3566 at 4158.284 kb on + strand, within Pf6N2E2_3566 at 4158.285 kb on - strand, within Pf6N2E2_3566 at 4158.315 kb on - strand, within Pf6N2E2_3566 at 4158.416 kb on + strand, within Pf6N2E2_3566 at 4158.417 kb on - strand, within Pf6N2E2_3566 at 4158.417 kb on - strand, within Pf6N2E2_3566 at 4158.417 kb on - strand, within Pf6N2E2_3566 at 4158.417 kb on - strand, within Pf6N2E2_3566 at 4158.417 kb on - strand, within Pf6N2E2_3566 at 4158.649 kb on + strand, within Pf6N2E2_3566 at 4158.649 kb on + strand, within Pf6N2E2_3566 at 4158.649 kb on + strand, within Pf6N2E2_3566 at 4159.036 kb on + strand at 4159.037 kb on - strand at 4159.098 kb on + strand at 4159.143 kb on + strand at 4159.144 kb on - strand at 4159.144 kb on - strand at 4159.144 kb on - strand at 4159.144 kb on - strand at 4159.144 kb on - strand at 4159.144 kb on - strand at 4159.167 kb on + strand at 4159.168 kb on - strand at 4159.168 kb on - strand at 4159.210 kb on - strand at 4159.245 kb on - strand at 4159.247 kb on + strand at 4159.248 kb on - strand at 4159.248 kb on - strand at 4159.269 kb on + strand at 4159.270 kb on - strand at 4159.288 kb on + strand at 4159.289 kb on - strand at 4159.289 kb on - strand at 4159.289 kb on - strand at 4159.350 kb on + strand at 4159.350 kb on + strand at 4159.350 kb on + strand at 4159.351 kb on - strand at 4159.418 kb on - strand, within Pf6N2E2_3567 at 4159.448 kb on + strand, within Pf6N2E2_3567 at 4159.449 kb on - strand, within Pf6N2E2_3567 at 4159.514 kb on + strand, within Pf6N2E2_3567 at 4159.684 kb on + strand, within Pf6N2E2_3567 at 4159.685 kb on - strand, within Pf6N2E2_3567 at 4159.872 kb on + strand at 4159.872 kb on + strand at 4159.873 kb on - strand at 4159.873 kb on - strand at 4159.873 kb on - strand at 4159.873 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 4,155,901 - Pf6N2E2_3564 0.70 -0.6 4,155,916 - Pf6N2E2_3564 0.71 -2.9 4,155,916 - Pf6N2E2_3564 0.71 -1.3 4,155,965 + Pf6N2E2_3564 0.77 -0.3 4,155,965 + Pf6N2E2_3564 0.77 -0.2 4,156,042 - Pf6N2E2_3564 0.85 +0.6 4,156,182 + -0.2 4,156,183 - -0.9 4,156,183 - +0.2 4,156,258 + -0.1 4,156,258 + +0.2 4,156,267 + -0.2 4,156,268 - +1.2 4,156,268 - +0.4 4,156,268 - +0.9 4,156,268 - -0.2 4,156,268 - -0.3 4,156,327 - Pf6N2E2_3565 0.16 +1.1 4,156,402 - Pf6N2E2_3565 0.30 +1.0 4,156,413 + Pf6N2E2_3565 0.32 -0.3 4,156,413 + Pf6N2E2_3565 0.32 +0.2 4,156,414 - Pf6N2E2_3565 0.32 +0.4 4,156,414 - Pf6N2E2_3565 0.32 -0.2 4,156,414 - Pf6N2E2_3565 0.32 +2.4 4,156,578 + Pf6N2E2_3565 0.63 +1.2 4,156,578 + Pf6N2E2_3565 0.63 -0.4 4,156,578 + Pf6N2E2_3565 0.63 -0.3 4,156,578 + Pf6N2E2_3565 0.63 -0.1 4,156,578 + Pf6N2E2_3565 0.63 +1.2 4,156,578 + Pf6N2E2_3565 0.63 -1.8 4,156,579 - Pf6N2E2_3565 0.63 -1.9 4,156,611 + Pf6N2E2_3565 0.69 -0.1 4,156,611 + Pf6N2E2_3565 0.69 +1.4 4,156,611 + Pf6N2E2_3565 0.69 -0.2 4,156,612 - Pf6N2E2_3565 0.70 -1.4 4,156,612 - Pf6N2E2_3565 0.70 +0.1 4,156,612 - Pf6N2E2_3565 0.70 +0.0 4,156,668 + Pf6N2E2_3565 0.80 +0.5 4,156,668 + Pf6N2E2_3565 0.80 +0.2 4,156,668 + Pf6N2E2_3565 0.80 +0.2 4,156,668 + Pf6N2E2_3565 0.80 +0.2 4,156,668 + Pf6N2E2_3565 0.80 +0.2 4,156,668 + Pf6N2E2_3565 0.80 +0.6 4,156,669 - Pf6N2E2_3565 0.80 -1.2 4,156,773 + +0.6 4,156,774 - -0.6 4,156,779 + -0.1 4,156,779 + -0.5 4,156,779 + -1.1 4,156,780 - -1.0 4,156,785 + +0.4 4,157,102 + Pf6N2E2_3566 0.10 +1.4 4,157,103 - Pf6N2E2_3566 0.11 +0.2 4,157,103 - Pf6N2E2_3566 0.11 +0.1 4,157,103 - Pf6N2E2_3566 0.11 -0.4 4,157,103 - Pf6N2E2_3566 0.11 +0.1 4,157,103 - Pf6N2E2_3566 0.11 -1.2 4,157,103 - Pf6N2E2_3566 0.11 -0.5 4,157,103 - Pf6N2E2_3566 0.11 -0.3 4,157,103 - Pf6N2E2_3566 0.11 +0.4 4,157,103 - Pf6N2E2_3566 0.11 +0.5 4,157,201 + Pf6N2E2_3566 0.15 +1.0 4,157,201 + Pf6N2E2_3566 0.15 +0.0 4,157,202 - Pf6N2E2_3566 0.15 +0.0 4,157,202 - Pf6N2E2_3566 0.15 +0.1 4,157,202 - Pf6N2E2_3566 0.15 -0.4 4,157,202 - Pf6N2E2_3566 0.15 +0.3 4,157,202 - Pf6N2E2_3566 0.15 +0.1 4,157,202 - Pf6N2E2_3566 0.15 -1.1 4,157,202 - Pf6N2E2_3566 0.15 -0.3 4,157,327 + Pf6N2E2_3566 0.21 +1.2 4,157,327 + Pf6N2E2_3566 0.21 +0.7 4,157,327 + Pf6N2E2_3566 0.21 +0.1 4,157,327 + Pf6N2E2_3566 0.21 +0.8 4,157,327 + Pf6N2E2_3566 0.21 -1.1 4,157,328 - Pf6N2E2_3566 0.21 -0.6 4,157,328 - Pf6N2E2_3566 0.21 -0.3 4,157,328 - Pf6N2E2_3566 0.21 -1.7 4,157,328 - Pf6N2E2_3566 0.21 +0.5 4,157,670 + Pf6N2E2_3566 0.38 +0.3 4,157,671 - Pf6N2E2_3566 0.38 -1.2 4,157,671 - Pf6N2E2_3566 0.38 +0.5 4,157,671 - Pf6N2E2_3566 0.38 -0.1 4,157,671 - Pf6N2E2_3566 0.38 +0.1 4,157,671 - Pf6N2E2_3566 0.38 +0.6 4,157,671 - Pf6N2E2_3566 0.38 +0.4 4,157,672 + Pf6N2E2_3566 0.38 -0.6 4,157,672 + Pf6N2E2_3566 0.38 +0.3 4,157,672 + Pf6N2E2_3566 0.38 -1.7 4,157,672 + Pf6N2E2_3566 0.38 +0.1 4,157,672 + Pf6N2E2_3566 0.38 -0.9 4,157,672 + Pf6N2E2_3566 0.38 -0.3 4,157,672 + Pf6N2E2_3566 0.38 +2.2 4,157,673 - Pf6N2E2_3566 0.38 -0.9 4,157,673 - Pf6N2E2_3566 0.38 +0.4 4,157,673 - Pf6N2E2_3566 0.38 +0.2 4,157,673 - Pf6N2E2_3566 0.38 -0.8 4,157,673 - Pf6N2E2_3566 0.38 -0.9 4,157,673 - Pf6N2E2_3566 0.38 -1.5 4,157,700 + Pf6N2E2_3566 0.39 -0.2 4,157,930 + Pf6N2E2_3566 0.50 -0.9 4,157,930 + Pf6N2E2_3566 0.50 +1.2 4,157,930 + Pf6N2E2_3566 0.50 -1.0 4,157,930 + Pf6N2E2_3566 0.50 -0.5 4,157,930 + Pf6N2E2_3566 0.50 +0.3 4,157,931 - Pf6N2E2_3566 0.50 -0.3 4,157,931 - Pf6N2E2_3566 0.50 -0.3 4,157,931 - Pf6N2E2_3566 0.50 -0.7 4,157,949 + Pf6N2E2_3566 0.51 +0.8 4,158,284 + Pf6N2E2_3566 0.67 -0.3 4,158,284 + Pf6N2E2_3566 0.67 +0.4 4,158,284 + Pf6N2E2_3566 0.67 -0.1 4,158,284 + Pf6N2E2_3566 0.67 -1.6 4,158,285 - Pf6N2E2_3566 0.67 +0.3 4,158,315 - Pf6N2E2_3566 0.69 +0.4 4,158,416 + Pf6N2E2_3566 0.74 +0.9 4,158,417 - Pf6N2E2_3566 0.74 -1.3 4,158,417 - Pf6N2E2_3566 0.74 -1.1 4,158,417 - Pf6N2E2_3566 0.74 +0.3 4,158,417 - Pf6N2E2_3566 0.74 -0.2 4,158,417 - Pf6N2E2_3566 0.74 +1.2 4,158,649 + Pf6N2E2_3566 0.85 +0.1 4,158,649 + Pf6N2E2_3566 0.85 +0.9 4,158,649 + Pf6N2E2_3566 0.85 +0.8 4,159,036 + -0.5 4,159,037 - +0.7 4,159,098 + +0.8 4,159,143 + -0.0 4,159,144 - -0.6 4,159,144 - +0.5 4,159,144 - +0.5 4,159,144 - +0.5 4,159,144 - +0.4 4,159,144 - +1.0 4,159,167 + -2.6 4,159,168 - -0.0 4,159,168 - -0.6 4,159,210 - -0.4 4,159,245 - +0.1 4,159,247 + -0.3 4,159,248 - +0.1 4,159,248 - +1.3 4,159,269 + -1.7 4,159,270 - -0.1 4,159,288 + -2.2 4,159,289 - +0.7 4,159,289 - -0.4 4,159,289 - +0.2 4,159,350 + -1.1 4,159,350 + +0.3 4,159,350 + -1.6 4,159,351 - -0.7 4,159,418 - Pf6N2E2_3567 0.19 -0.1 4,159,448 + Pf6N2E2_3567 0.27 -0.3 4,159,449 - Pf6N2E2_3567 0.27 -0.0 4,159,514 + Pf6N2E2_3567 0.42 -1.0 4,159,684 + Pf6N2E2_3567 0.83 -0.2 4,159,685 - Pf6N2E2_3567 0.83 -1.0 4,159,872 + +0.5 4,159,872 + -1.2 4,159,873 - -0.9 4,159,873 - -0.9 4,159,873 - -2.3 4,159,873 - +0.3
Or see this region's nucleotide sequence