Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3053 and Pf6N2E2_3054 are separated by 149 nucleotides Pf6N2E2_3054 and Pf6N2E2_3055 are separated by 194 nucleotides Pf6N2E2_3055 and Pf6N2E2_3056 are separated by 17 nucleotides
Pf6N2E2_3053 - Acetyl-coenzyme A carboxyl transferase alpha chain (EC 6.4.1.2), at 3,536,772 to 3,537,719
_3053
Pf6N2E2_3054 - DNA polymerase III alpha subunit (EC 2.7.7.7), at 3,537,869 to 3,541,390
_3054
Pf6N2E2_3055 - Ribonuclease HII (EC 3.1.26.4), at 3,541,585 to 3,542,217
_3055
Pf6N2E2_3056 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 3,542,235 to 3,543,365
_3056
Position (kb)
3537
3538
3539
3540
3541
3542 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3541.391 kb on - strand at 3541.405 kb on - strand at 3541.449 kb on - strand at 3541.449 kb on - strand at 3541.537 kb on + strand at 3541.628 kb on + strand at 3541.629 kb on - strand at 3541.629 kb on - strand at 3541.749 kb on - strand, within Pf6N2E2_3055 at 3541.882 kb on - strand, within Pf6N2E2_3055 at 3542.036 kb on + strand, within Pf6N2E2_3055 at 3542.221 kb on + strand at 3542.221 kb on + strand at 3542.221 kb on + strand at 3542.222 kb on - strand at 3542.222 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 3,541,391 - +1.6 3,541,405 - +0.8 3,541,449 - +2.2 3,541,449 - -0.1 3,541,537 + -1.3 3,541,628 + +0.2 3,541,629 - +0.5 3,541,629 - -1.8 3,541,749 - Pf6N2E2_3055 0.26 +0.5 3,541,882 - Pf6N2E2_3055 0.47 +0.9 3,542,036 + Pf6N2E2_3055 0.71 -1.2 3,542,221 + +1.1 3,542,221 + -1.5 3,542,221 + -0.4 3,542,222 - +0.3 3,542,222 - -0.0
Or see this region's nucleotide sequence