Experiment: Fusidic 0.4 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1015 and Pf6N2E2_1016 are separated by 48 nucleotides Pf6N2E2_1016 and Pf6N2E2_1017 overlap by 4 nucleotides Pf6N2E2_1017 and Pf6N2E2_1018 are separated by 124 nucleotides
Pf6N2E2_1015 - Inositol transport system sugar-binding protein, at 1,184,934 to 1,185,857
_1015
Pf6N2E2_1016 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 1,185,906 to 1,187,060
_1016
Pf6N2E2_1017 - small molecule metabolism, at 1,187,057 to 1,187,905
_1017
Pf6N2E2_1018 - putative DNA-binding protein, at 1,188,030 to 1,189,055
_1018
Position (kb)
1185
1186
1187
1188 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1184.956 kb on + strand at 1184.957 kb on - strand at 1184.980 kb on + strand at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.260 kb on + strand, within Pf6N2E2_1015 at 1185.261 kb on - strand, within Pf6N2E2_1015 at 1185.343 kb on - strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.535 kb on + strand, within Pf6N2E2_1015 at 1185.666 kb on + strand, within Pf6N2E2_1015 at 1185.733 kb on + strand, within Pf6N2E2_1015 at 1185.734 kb on - strand, within Pf6N2E2_1015 at 1185.835 kb on + strand at 1185.901 kb on + strand at 1185.902 kb on - strand at 1185.927 kb on + strand at 1185.958 kb on + strand at 1185.958 kb on + strand at 1186.110 kb on + strand, within Pf6N2E2_1016 at 1186.145 kb on - strand, within Pf6N2E2_1016 at 1186.336 kb on + strand, within Pf6N2E2_1016 at 1186.377 kb on + strand, within Pf6N2E2_1016 at 1186.377 kb on + strand, within Pf6N2E2_1016 at 1186.378 kb on - strand, within Pf6N2E2_1016 at 1186.378 kb on - strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.381 kb on + strand, within Pf6N2E2_1016 at 1186.382 kb on - strand at 1186.382 kb on - strand, within Pf6N2E2_1016 at 1186.382 kb on - strand, within Pf6N2E2_1016 at 1186.466 kb on - strand, within Pf6N2E2_1016 at 1186.534 kb on + strand, within Pf6N2E2_1016 at 1186.651 kb on + strand, within Pf6N2E2_1016 at 1186.651 kb on + strand, within Pf6N2E2_1016 at 1186.652 kb on - strand, within Pf6N2E2_1016 at 1186.652 kb on - strand, within Pf6N2E2_1016 at 1186.654 kb on + strand, within Pf6N2E2_1016 at 1186.655 kb on - strand, within Pf6N2E2_1016 at 1186.655 kb on - strand, within Pf6N2E2_1016 at 1186.927 kb on + strand, within Pf6N2E2_1016 at 1186.928 kb on - strand, within Pf6N2E2_1016 at 1186.928 kb on - strand, within Pf6N2E2_1016 at 1186.928 kb on - strand, within Pf6N2E2_1016 at 1187.430 kb on + strand, within Pf6N2E2_1017 at 1187.865 kb on + strand at 1187.865 kb on + strand at 1187.885 kb on - strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.921 kb on + strand at 1187.922 kb on - strand at 1187.968 kb on + strand at 1187.969 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.4 mg/ml remove 1,184,956 + +0.6 1,184,957 - +1.4 1,184,980 + +0.3 1,185,260 + Pf6N2E2_1015 0.35 -1.3 1,185,260 + Pf6N2E2_1015 0.35 -0.9 1,185,261 - Pf6N2E2_1015 0.35 -2.5 1,185,343 - Pf6N2E2_1015 0.44 +0.0 1,185,535 + Pf6N2E2_1015 0.65 +0.7 1,185,535 + Pf6N2E2_1015 0.65 +0.9 1,185,535 + Pf6N2E2_1015 0.65 -0.8 1,185,666 + Pf6N2E2_1015 0.79 -0.7 1,185,733 + Pf6N2E2_1015 0.86 +0.1 1,185,734 - Pf6N2E2_1015 0.87 -1.1 1,185,835 + +2.3 1,185,901 + +0.3 1,185,902 - -0.5 1,185,927 + +0.8 1,185,958 + +0.9 1,185,958 + -1.1 1,186,110 + Pf6N2E2_1016 0.18 +0.7 1,186,145 - Pf6N2E2_1016 0.21 +0.6 1,186,336 + Pf6N2E2_1016 0.37 +0.2 1,186,377 + Pf6N2E2_1016 0.41 +0.1 1,186,377 + Pf6N2E2_1016 0.41 +0.3 1,186,378 - Pf6N2E2_1016 0.41 -0.5 1,186,378 - Pf6N2E2_1016 0.41 +0.8 1,186,381 + Pf6N2E2_1016 0.41 +0.3 1,186,381 + Pf6N2E2_1016 0.41 -0.2 1,186,381 + Pf6N2E2_1016 0.41 +1.1 1,186,382 - -1.7 1,186,382 - Pf6N2E2_1016 0.41 +0.1 1,186,382 - Pf6N2E2_1016 0.41 +0.4 1,186,466 - Pf6N2E2_1016 0.48 +1.4 1,186,534 + Pf6N2E2_1016 0.54 -0.5 1,186,651 + Pf6N2E2_1016 0.65 -0.8 1,186,651 + Pf6N2E2_1016 0.65 +0.1 1,186,652 - Pf6N2E2_1016 0.65 +0.6 1,186,652 - Pf6N2E2_1016 0.65 +0.7 1,186,654 + Pf6N2E2_1016 0.65 -0.8 1,186,655 - Pf6N2E2_1016 0.65 +1.0 1,186,655 - Pf6N2E2_1016 0.65 +0.9 1,186,927 + Pf6N2E2_1016 0.88 +1.1 1,186,928 - Pf6N2E2_1016 0.88 +1.2 1,186,928 - Pf6N2E2_1016 0.88 -0.1 1,186,928 - Pf6N2E2_1016 0.88 +0.2 1,187,430 + Pf6N2E2_1017 0.44 +1.4 1,187,865 + -0.5 1,187,865 + +0.3 1,187,885 - +0.4 1,187,921 + -0.6 1,187,921 + -0.4 1,187,921 + -1.6 1,187,921 + +0.1 1,187,921 + +0.2 1,187,921 + +1.9 1,187,921 + +0.7 1,187,921 + +1.1 1,187,922 - +2.3 1,187,968 + +1.8 1,187,969 - -0.1
Or see this region's nucleotide sequence