Experiment: L-Malic (C) with ammonium chloride; anaerobic; 3 days
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HSERO_RS01915 and HSERO_RS01920 are separated by 3 nucleotides HSERO_RS01920 and HSERO_RS01925 are separated by 41 nucleotides HSERO_RS01925 and HSERO_RS01930 are separated by 95 nucleotides HSERO_RS01930 and HSERO_RS01935 are separated by 134 nucleotides
HSERO_RS01915: HSERO_RS01915 - membrane protein, at 421,059 to 422,348
_RS01915
HSERO_RS01920: HSERO_RS01920 - 50S ribosomal protein L11 methyltransferase, at 422,352 to 423,266
_RS01920
HSERO_RS01925: HSERO_RS01925 - acetyl-CoA carboxylase biotin carboxylase subunit, at 423,308 to 424,687
_RS01925
HSERO_RS01930: HSERO_RS01930 - acetyl-CoA carboxylase, at 424,783 to 425,256
_RS01930
HSERO_RS01935: HSERO_RS01935 - 3-dehydroquinate dehydratase, at 425,391 to 425,828
_RS01935
Position (kb) 423
424
425 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 422.413 kb on - strand at 422.510 kb on + strand, within HSERO_RS01920 at 422.518 kb on - strand, within HSERO_RS01920 at 422.609 kb on + strand, within HSERO_RS01920 at 422.751 kb on + strand, within HSERO_RS01920 at 422.972 kb on - strand, within HSERO_RS01920 at 423.001 kb on + strand, within HSERO_RS01920 at 423.014 kb on - strand, within HSERO_RS01920 at 425.397 kb on - strand at 425.456 kb on - strand, within HSERO_RS01935 at 425.573 kb on - strand, within HSERO_RS01935
Per-strain Table
Position Strand Gene LocusTag Fraction L-Malic (C) with ammonium chloride; anaerobic; 3 days remove 422,413 - -3.4 422,510 + HSERO_RS01920 0.17 -1.4 422,518 - HSERO_RS01920 0.18 -0.3 422,609 + HSERO_RS01920 0.28 -1.3 422,751 + HSERO_RS01920 0.44 -1.2 422,972 - HSERO_RS01920 0.68 -1.6 423,001 + HSERO_RS01920 0.71 -0.5 423,014 - HSERO_RS01920 0.72 -0.4 425,397 - -0.2 425,456 - HSERO_RS01935 0.15 -4.0 425,573 - HSERO_RS01935 0.42 -4.1
Or see this region's nucleotide sequence