Experiment: Fusidic 0.3 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_784 and Pf6N2E2_785 are separated by 37 nucleotides Pf6N2E2_785 and Pf6N2E2_786 are separated by 109 nucleotides Pf6N2E2_786 and Pf6N2E2_787 are separated by 253 nucleotides
Pf6N2E2_784 - Outer membrane usher protein FIMD, at 897,590 to 900,085
_784
Pf6N2E2_785 - P pilus assembly protein, chaperone PapD, at 900,123 to 900,884
_785
Pf6N2E2_786 - Type-1 fimbrial protein, A chain precursor, at 900,994 to 901,542
_786
Pf6N2E2_787 - YD repeat protein, at 901,796 to 906,883
_787
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 900.098 kb on + strand at 900.278 kb on + strand, within Pf6N2E2_785 at 900.279 kb on - strand, within Pf6N2E2_785 at 900.352 kb on + strand, within Pf6N2E2_785 at 900.352 kb on + strand, within Pf6N2E2_785 at 900.352 kb on + strand, within Pf6N2E2_785 at 900.479 kb on + strand, within Pf6N2E2_785 at 900.535 kb on + strand, within Pf6N2E2_785 at 900.535 kb on + strand, within Pf6N2E2_785 at 900.699 kb on + strand, within Pf6N2E2_785 at 900.699 kb on + strand, within Pf6N2E2_785 at 900.699 kb on + strand, within Pf6N2E2_785 at 900.699 kb on + strand, within Pf6N2E2_785 at 900.699 kb on + strand, within Pf6N2E2_785 at 900.700 kb on - strand, within Pf6N2E2_785 at 900.700 kb on - strand, within Pf6N2E2_785 at 900.701 kb on + strand, within Pf6N2E2_785 at 900.892 kb on + strand at 900.958 kb on + strand at 901.116 kb on - strand, within Pf6N2E2_786 at 901.255 kb on - strand, within Pf6N2E2_786 at 901.377 kb on - strand, within Pf6N2E2_786 at 901.377 kb on - strand, within Pf6N2E2_786 at 901.377 kb on - strand, within Pf6N2E2_786 at 901.377 kb on - strand, within Pf6N2E2_786 at 901.377 kb on - strand, within Pf6N2E2_786 at 901.638 kb on + strand at 901.644 kb on + strand at 901.644 kb on + strand at 901.877 kb on + strand at 902.020 kb on + strand at 902.030 kb on + strand at 902.031 kb on - strand at 902.042 kb on - strand at 902.042 kb on - strand at 902.051 kb on + strand at 902.052 kb on - strand at 902.052 kb on - strand at 902.052 kb on - strand at 902.055 kb on - strand at 902.176 kb on - strand at 902.176 kb on - strand at 902.207 kb on + strand at 902.208 kb on - strand at 902.275 kb on + strand at 902.276 kb on - strand at 902.276 kb on - strand at 902.276 kb on - strand at 902.303 kb on + strand at 902.304 kb on - strand at 902.304 kb on - strand at 902.304 kb on - strand at 902.305 kb on + strand, within Pf6N2E2_787 at 902.305 kb on + strand, within Pf6N2E2_787 at 902.306 kb on - strand, within Pf6N2E2_787 at 902.306 kb on - strand, within Pf6N2E2_787 at 902.311 kb on - strand, within Pf6N2E2_787 at 902.311 kb on - strand, within Pf6N2E2_787 at 902.311 kb on - strand, within Pf6N2E2_787 at 902.335 kb on + strand, within Pf6N2E2_787 at 902.335 kb on + strand, within Pf6N2E2_787 at 902.360 kb on + strand, within Pf6N2E2_787 at 902.361 kb on - strand, within Pf6N2E2_787 at 902.389 kb on + strand, within Pf6N2E2_787 at 902.419 kb on + strand, within Pf6N2E2_787 at 902.419 kb on + strand, within Pf6N2E2_787 at 902.420 kb on - strand, within Pf6N2E2_787 at 902.439 kb on - strand, within Pf6N2E2_787 at 902.457 kb on - strand, within Pf6N2E2_787 at 902.457 kb on - strand, within Pf6N2E2_787 at 902.457 kb on - strand, within Pf6N2E2_787 at 902.457 kb on - strand, within Pf6N2E2_787 at 902.519 kb on - strand, within Pf6N2E2_787 at 902.519 kb on - strand, within Pf6N2E2_787
Per-strain Table
Position Strand Gene LocusTag Fraction Fusidic 0.3 mg/ml remove 900,098 + +0.9 900,278 + Pf6N2E2_785 0.20 +0.9 900,279 - Pf6N2E2_785 0.20 -1.9 900,352 + Pf6N2E2_785 0.30 -0.7 900,352 + Pf6N2E2_785 0.30 +0.3 900,352 + Pf6N2E2_785 0.30 -0.4 900,479 + Pf6N2E2_785 0.47 -0.7 900,535 + Pf6N2E2_785 0.54 +0.9 900,535 + Pf6N2E2_785 0.54 -0.6 900,699 + Pf6N2E2_785 0.76 +0.4 900,699 + Pf6N2E2_785 0.76 +1.3 900,699 + Pf6N2E2_785 0.76 -0.9 900,699 + Pf6N2E2_785 0.76 -0.1 900,699 + Pf6N2E2_785 0.76 -1.2 900,700 - Pf6N2E2_785 0.76 -0.2 900,700 - Pf6N2E2_785 0.76 -0.1 900,701 + Pf6N2E2_785 0.76 -0.8 900,892 + +0.3 900,958 + +0.6 901,116 - Pf6N2E2_786 0.22 -0.5 901,255 - Pf6N2E2_786 0.48 -1.3 901,377 - Pf6N2E2_786 0.70 -0.4 901,377 - Pf6N2E2_786 0.70 -0.4 901,377 - Pf6N2E2_786 0.70 -0.9 901,377 - Pf6N2E2_786 0.70 -0.4 901,377 - Pf6N2E2_786 0.70 -0.8 901,638 + -1.1 901,644 + +0.9 901,644 + -0.1 901,877 + -0.9 902,020 + -0.7 902,030 + +0.1 902,031 - -0.2 902,042 - -0.4 902,042 - -0.9 902,051 + +0.4 902,052 - +0.6 902,052 - +0.7 902,052 - -0.5 902,055 - -0.8 902,176 - +0.5 902,176 - +1.0 902,207 + +0.4 902,208 - +0.1 902,275 + -1.6 902,276 - -2.1 902,276 - +1.4 902,276 - +0.7 902,303 + +1.0 902,304 - -1.5 902,304 - +1.1 902,304 - -0.6 902,305 + Pf6N2E2_787 0.10 +0.4 902,305 + Pf6N2E2_787 0.10 +0.7 902,306 - Pf6N2E2_787 0.10 -0.8 902,306 - Pf6N2E2_787 0.10 -0.7 902,311 - Pf6N2E2_787 0.10 +1.7 902,311 - Pf6N2E2_787 0.10 -0.1 902,311 - Pf6N2E2_787 0.10 +2.0 902,335 + Pf6N2E2_787 0.11 +1.3 902,335 + Pf6N2E2_787 0.11 -1.6 902,360 + Pf6N2E2_787 0.11 +1.4 902,361 - Pf6N2E2_787 0.11 -0.9 902,389 + Pf6N2E2_787 0.12 -0.4 902,419 + Pf6N2E2_787 0.12 -0.1 902,419 + Pf6N2E2_787 0.12 +0.4 902,420 - Pf6N2E2_787 0.12 -0.0 902,439 - Pf6N2E2_787 0.13 -0.2 902,457 - Pf6N2E2_787 0.13 +0.3 902,457 - Pf6N2E2_787 0.13 -0.4 902,457 - Pf6N2E2_787 0.13 +1.3 902,457 - Pf6N2E2_787 0.13 -0.0 902,519 - Pf6N2E2_787 0.14 +1.3 902,519 - Pf6N2E2_787 0.14 +0.7
Or see this region's nucleotide sequence